Optimal Timing for Corticosteroid Therapy in Idiopathic Granulomatous Mastitis: A Retrospective Analysis Highlighting Early Intervention Efficacy.

Background: Idiopathic granulomatous mastitis (IGM) is a chronic breast condition known for its aggressive nature and tendency for persistence and recurrence. Steroids are commonly used as the first-line treatment for IGM, but issues such as the optimal timing, and duration of treatment remain debated.

Methods: We retrospectively analyzed 343 IGM cases treated at the Third People's Hospital of Chengdu from September 2012 to September 2023.

[Cloning of the gene encoding a key enzyme involved in production of glycerol in Candida glycerinogenes].

Candida glycerinogenes WL2002-5, an excellent glycerol producer, has been used for industrial scale fermentation of glycerol by an aerobic process. However, our knowledge about glycerol biosynthesis at the molecular level and genetic background of this yeast species lags far behind those of model yeasts such as Saccharomyces cerevisiae et al. In this report, inverse primers, in conjunction with degenerated primers, were used to amplify the NAD+-dependent glycerol 3-phosphate dehydrogenase (GPD) encoding gene from C.

Bioconversion of lovastatin to a novel statin by Amycolatopsis sp.

3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase catalyzes the conversion of HMG-CoA to mevalonic acid, which plays a significant role in cholesterol synthesis. Several statins, inhibitors of HMG-CoA reductase, can be synthesized and converted by microorganisms. Among 700 strains obtained from culture collections, one strain could convert lovastatin to a novel statin, wuxistatin.

[Construction of recombinant Saccharomyces cerevisiae producing 1,3-propanediol by one step method].

1,3-Propanediol is one of the most important industrial chemicals for its highly desired properties and its wide applications as a key component of an emerging polymer business. Biological production of 1,3-propanediol has been a novel and competitive way. In our previous job, the gene dahB encoding for glycerol dehydratase from Klebsiella and the gene yqhD encoding for 1,3-propanediol oxidoreductase isoenzyme from E.

Construction of a flocculating yeast for fuel ethanol production.

The expression vector pYX212 harboring FLO1 gene and kanMX gene was transformed into Saccharomyces. cerevisiae ZWA46. The transformant, ZWA46-F2, showed strong and stable flocculation ability during 20 serial batch cultivations.

[Stimulatory effects of some amino acids on glycerol production by Candida glycerinogenes].

By using some intermediate metabolites in EMP pathway and TCA cycle as the control, the effects of amino acid supplements on glycerol production by Candida glycerinogenes in shake-flask fermentations with urea as nitrogen resource were investigated. The results showed that ten kinds of amino acids, including L-glutamic acid, L-glutamine, L-aspartic acid, L-asparagine, L-glycin, L-lysine, L-tyrosine, L-proline, L-histidine, and L-serine, had strong promotional effects on glycerol production; The optimal concentrations of these amino acids were 0.40, 0.

[Construction of novel recombinant Escherichia coli capable of producing 1,3-propanediol].

The 1,3-propanediol oxidoreductase isoenzyme encoding gene (yqhD) from E. coli was amplified by PCR. yqhD was inserted in pEtac to yield the recombinant expression vector pEtac-yqhD.

Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis.

An extracellular alkaline protease produced by Bacillus licheniformis AP-1 was purified 76-fold, yielding a single 28 kDa band on SDS-PAGE. It was optimally active at pH 11 and at 60 degrees C (assayed over 10 min). The protease was completely inhibited by phenylmethylsulfonyl fluoride and diodopropyl fluorophosphate, with little increase upon Ca2+ and Mg2+ addition.