Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig.

Olfactory receptors (ORs) are members of the transmembrane G protein-coupled receptor superfamily, playing a crucial role in odor recognition, which further mediates crucial biological processes in mammals. In sows, androstenone can trigger sexual behaviors through olfaction, but the underlying mechanism remains to be explored. To efficiently and accurately screen pig olfactory receptors responding to androstenone and the key structure determinant, we adapted the high-throughput RNA-seq strategy to screen the altered genes upon androstenone treatment in the olfactory epithelium of pigs, yielding 1397 downregulated genes.

Genetic Variation for Wild Populations of the Rare and Endangered Plant Based on Double-Digest Restriction Site-Associated DNA Sequencing.

is an endangered tree species, and detecting its genetic diversity can reveal the mechanisms of endangerment, providing references for the conservation of genetic resources. Samples of 137 trees across seven populations within Fujian Province were collected and sequenced using double-digest restriction site-associated DNA (ddRAD-seq). A total of 3,687,189 single-nucleotide polymorphisms (SNPs) were identified, and 15,158 high-quality SNPs were obtained after filtering.

Three-Dimensional Printable Magnetic Hydrogels with Adjustable Stiffness and Adhesion for Magnetic Actuation and Magnetic Hyperthermia Applications.

Stimuli-responsive hydrogels hold immense promise for biomedical applications, but conventional gelation processes often struggle to achieve the precision and complexity required for advanced functionalities such as soft robotics, targeted drug delivery, and tissue engineering. This study introduces a class of 3D-printable magnetic hydrogels with tunable stiffness, adhesion, and magnetic responsiveness, prepared through a simple and efficient "one-pot" method. This approach enables precise control over the hydrogel's mechanical properties, with an elastic modulus ranging from 43 kPa to 277 kPa, tensile strength from 93 kPa to 421 kPa, and toughness from 243 kJ/m to 1400 kJ/m, achieved by modulating the concentrations of acrylamide (AM) and FeO nanoparticles.

From Acceptance to Dependence: Exploring Influences of Smart Healthcare on Continuous Use Intention of Mobile Health Services Among Older Adults with Chronic Illnesses in China.

With the acceleration of the aging process in China, chronic diseases have become one of the main health threats for older adults, creating significant pressure on society and the healthcare system. As information technology and artificial intelligence advance rapidly, smart health services have become readily accessible. However, utilization rates among the older adults, especially those with chronic illnesses, remain low, preventing them from fully benefiting from these advanced technologies.

The Development and Validation of the Adolescent Problematic Gaming Scale (PGS-Adolescent).

This study aims to develop and validate the Adolescent Problematic Gaming Scale (PGS-Adolescent). Following established scientific protocols, we developed an initial version of the PGS-Adolescent scale and validated it using data from 448 valid survey responses collected from adolescents in China. The dataset was split into two parts: 225 responses were allocated for exploratory factor analysis (EFA), and 223 for confirmatory factor analysis (CFA).

A Bio-Inspired Visual Neural Model for Robustly and Steadily Detecting Motion Directions of Translating Objects Against Variable Contrast in the Figure-Ground and Noise Interference.

(1) Background: At present, the bio-inspired visual neural models have made significant achievements in detecting the motion direction of the translating object. Variable contrast in the figure-ground and environmental noise interference, however, have a strong influence on the existing model. The responses of the lobula plate tangential cell (LPTC) neurons of Drosophila are robust and stable in the face of variable contrast in the figure-ground and environmental noise interference, which provides an excellent paradigm for addressing these challenges.

Analysis of the Spatiotemporal Heterogeneity and Influencing Factors of Regional Economic Resilience in China.

This study estimates regional economic resilience in China from 2000 to 2022, focusing on economic resistance resilience, recovery resilience, and reorientation resilience. The entropy method, kernel density estimation, and spatial Durbin model are applied to examine the spatiotemporal evolution and influencing factors. The results show significant spatial clustering, with stronger resilience in the east and weaker resilience in the west.

An Overview of Dentist-Patient Communication in Quality Dental Care.

Dentist-patient communication is at the core of providing quality dental care. This study aims to review the importance, challenges, strategies, and training of dentist-patient communication. The World Dental Federation (FDI) emphasizes the importance of effective communication between oral healthcare providers and patients as a critical component of high-quality care.

Motor Imagery EEG Classification Based on Multi-Domain Feature Rotation and Stacking Ensemble.

Background: Decoding motor intentions from electroencephalogram (EEG) signals is a critical component of motor imagery-based brain-computer interface (MI-BCIs). In traditional EEG signal classification, effectively utilizing the valuable information contained within the electroencephalogram is crucial.

Objectives: To further optimize the use of information from various domains, we propose a novel framework based on multi-domain feature rotation transformation and stacking ensemble for classifying MI tasks.

Older Fallers' Comprehensive Neuromuscular and Kinematic Alterations in Reactive Balance Control: Indicators of Balance Decline or Compensation? A Pilot Study.

: Falls and fall consequences in older adults are global health issues. Previous studies have compared postural sways or stepping strategies between older adults with and without fall histories to identify factors associated with falls. However, more in-depth neuromuscular/kinematic mechanisms have remained unclear.

Plays a Pivotal Role in Colorectal Cancer Progression via the Dual Regulation of Cell Cycle and Epithelial-Mesenchymal Transition.

Background: Cytoskeleton-associated protein 2 like () has been demonstrated to mediate the cell cycle in cancer cells. However, it is unknown whether CKAP2L impacts colorectal cancer (CRC). The purpose of this study was to investigate the role of in CRC.

Chitosan-Based Porous Carbon Materials with Built-In Lewis Acid Boron Sites for Enhanced CO Capture and Conversion via an Electron-Inducing Effect.

Electron-induced effects, which are prevalent in adsorption and heterogeneous catalytic reactions, can significantly influence the state and uptake of adsorbates. Here, we demonstrate the in situ doping of electron-deficient boron into the backbone of chitosan-based porous carbon materials. Despite a reduction in specific surface area, the resulting boron-doped porous carbons (NBPCs) exhibit an enhanced CO adsorption performance, with sample NBPC-10 achieving CO adsorption capacities of 7.

Construction and validation of a prediction model for arteriovenous fistula thrombosis in patients with AVF using Lasso regression.

Objective: The primary objective of this study is to develop and validate a high-risk model for Arteriovenous Fistula Thrombosis (AVFT) in patients undergoing autogenous arteriovenous fistula surgery for hemodialysis.

Methods: Retrospectively, we collected general information, clinical characteristics, laboratory examinations, and dialysis-related factors from a cohort of 1465 patients who received continuous arteriovenous fistula surgery at the Hemodialysis Access Center of Sichuan Provincial People's Hospital between January 2019 and June 2022. The patients were randomly divided into a training set and a validation set in a 2:1 ratio.

A chromosome-scale genome assembly of the pioneer plant Stylosanthes angustifolia: insights into genome evolution and drought adaptation.

Background: Drought is a major limiting factor for plant survival and crop productivity. Stylosanthes angustifolia, a pioneer plant, exhibits remarkable drought tolerance, yet the molecular mechanisms driving its drought resistance remain largely unexplored.

Results: We present a chromosome-scale reference genome of S.

Mechanism of Water Freezing in Solutions: Solutes Affect the Formation of Critical Ice Nuclei.

The microscopic mechanisms by which solutes modulate water freezing are fundamental for controlling the freezing of various environmental and cryobiotic systems. Although our understanding of the initiation mechanisms of pure water freezing is becoming clearer, the microscopic pictures regarding ice nucleation in complex systems such as solutions still rely on theory assumption and empirical formulation. Here, we experimentally demonstrate that solutes modulate water freezing through affecting critical ice nucleus formation.

Oridonin Suppresses the Malignant Progression of Lung Cancer by Targeting S100A11.

Background: Lung cancer (LC) is the second most lethal cancer and efficient treatments are missing. Our understanding of the underlying pathogenic mechanisms remains limited. Oridonin is a compound extracted from the Chinese herb Rabdosia rubescens with anticancer properties.

Identification of serum tRNA-derived small RNAs biosignature for diagnosis of tuberculosis.

The tRNA-derived small RNAs (tsRNAs) are a new class of non coding RNAs, which are stable in body fluids and can be used as potential biomarkers for disease diagnosis. However, the exact value of tsRNAs in the diagnosis of tuberculosis (TB) is still unclear. The objective of the present study was to evaluate the performance of the serum tsRNAs biosignature to distinguish between active TB, healthy controls, latent TB infection, and other respiratory diseases.

Atrial Fibrillation Related Titin Truncation Is Associated With Atrial Myopathy in Patient-Derived Induced Pluripotent Stem Cell Disease Models.

Background: Protein-truncating mutations in the titin gene are associated with increased risk of atrial fibrillation. However, little is known about the underlying pathophysiology.

Methods: We identified a heterozygous titin truncating variant (TTNtv) in a patient with unexplained early onset atrial fibrillation and normal ventricular function.

Comparison of fluoride level between mother's milk and infant formula milk - a global systematic review and dental fluorosis risk assessment.

Dental Fluorosis (DF) is one of the negative outcomes of excessive fluoride (F) intake through food sources. This systematic review aimed to compare F content in two important food sources for infants, Mother's Milk (MoM) and Infant Formula (IF), and then evaluate the risk of DF related to F in those two types of food. For this purpose, 181 studies were initially found by searching the relevant keywords in widely recognized databases, including Google Scholar, Scopus, Science Direct, and PubMed.

Distal tibial fractures fixation using retrograde tibial intramedullary nail in high-risk patients: a retrospective study.

Distal tibial fractures are common lower-limb injuries and are generally associated with a high risk of postoperative complications, especially in patients with multiple medical comorbidities. This study sought to ascertain the efficacy of retrograde intramedullary tibial nails (RTN) for treating extra-articular distal tibial fractures in high-risk patients. Between January 2019 and December 2021, 13 patients considered at high risk for postoperative complications underwent RTN fixation.

Characteristics of Pediatric Allergic Rhinitis With Different Disease Severity.

Although numerous studies have focused on diagnostic biomarkers to help identify allergic rhinitis (AR), data on the characteristics of pediatric AR with different severity is limited. We aimed to compare the characteristics of pediatric AR with different severity. A total of 1054 children with AR were enrolled and classified into mild intermittent AR, mild persistent AR, moderate-to-severe intermittent AR, and moderate-to-severe persistent AR.

Bibliometric analysis on the top one hundred cited studies on gastrointestinal endoscopy.

Background: Gastrointestinal endoscopy has been widely used in the diagnosis and treatment of gastrointestinal diseases. A great many of studies on gastrointestinal endoscopy have been done.

Aim: To analyze the characteristics of top 100 cited articles on gastrointestinal endoscopy.

Synergistic defecation effects of subsp. BL-99 and fructooligosaccharide by modulating gut microbiota.

Introduction: Synbiotics have revealed the possibility of improving constipation through gut microbiota. The synergistic efficacy of subsp. lactis BL-99 (BL-99) and fructooligosaccharide (FOS) on constipation have not been investigated.

Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis and .

Object: Neuroinflammation mediated by microglia has emerged as a critical factor in ischemic stroke and neuronal damage. Gualou Guizhi Granule (GLGZG) has been shown to suppress inflammation in lipopolysaccharide (LPS)-activated microglia, though the underlying mechanisms and its protective effects against neuronal apoptosis remain unclear. This study aims to investigate how GLGZG regulates the Notch signaling pathway in microglia to reduce neuroinflammation and protect neurons from apoptosis.

Coexistence of immunoglobulin G4-related kidney disease and acute hematogenous disseminated pulmonary tuberculosis: a case report.

Background: Immunoglobulin G4-related disease (IgG4-RD) is an immune-mediated fibrous inflammatory disease. Recently, an association between IgG4-RD and tuberculosis (TB) has been reported.

Case Summary: We report a 56-year-old man complaining of a cough and poor appetite for 2 months and oliguria for 1 day.