Sativene-Related Sesquiterpenoids with Phytotoxic and Plant-Promoting Activities from the Plant Pathogenic Fungus Based on a Molecular Networking Strategy.

Sativene-related sesquiterpenoids including -sativene analogs are a large member of fungal secondary metabolites with phytotoxic and growth-promoting effects on different plants. In this report, a series of sativene-related sesquiterpenoids with diverse carbon skeletons (-, sativene/isosativene/-sativene/cyclosativene/-isosativene ring systems) were isolated from the plant pathogenic fungus based on a molecular networking strategy. The undescribed structures were elucidated based on NMR spectra, X-ray diffraction analysis, chemical derivation, and calculated electronic circular dichroism calculations.

UPLC-Q-TOF-MS/MS analysis of ophiobolins sesterterpenoids and bioactive analogs from Bipolaris eleusines.

In order to elucidate the mass fragmentation patterns and unveil more undescribed ophiobolin analogs, the mass fragmentation patterns of ophiobolins were analyzed based on UPLC-Q-TOF-MS/MS experiments. Different kinds of rearrangements (including McLafferty rearrangement) were the main cleavage patterns. Twenty-six (9-31) analogs were then tentatively characterized based on their mass analysis, and three undescribed ophiobolins (6-8) and a known analogue (5) were isolated in target.

UPLC-Q-TOF-MS/MS-Based Targeted Discovery of Chetomin Analogues from .

Chetocochliodin M () containing a rare cage-ring and chetocochliodin N () featuring an unusual piperazine-2,3-dione ring system together with known analogues chetomin (), chetoseminudin C (), chetocochliodin I (), and oidioperazine E () were targeted for purification from the fungus using a UPLC-Q-TOF-MS/MS approach. The structures of the new compounds were elucidated using HR-ESI-MS, NMR, and ECD spectra. Compounds , , and exhibited strong cytotoxic activities against A549 and HeLa cancer cell lines.