Publications by authors named "Hui-Li Lu"
Developing an accurate and reliable model for chromatographic separation that meets regulatory requirements and ensures consistency in model development remains challenging. In order to address this challenge, a standardized approach was proposed in this study with ion-exchange chromatography (IEC). The approach includes the following steps: liquid flow identification, system and column-specific parameters determination and validation, multi-component system identification, protein amount validation, steric mass action parameters determination and evaluation, and validation of the calibrated model's generalization ability.
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- Density functional calculations and microkinetic simulations were executed to analyze how acetylene transforms on various metal surfaces (Pd, Cu, Ag, Au, and their alloys), revealing a linear correlation in adsorption energies between alloy and pure metal surfaces.
- The study identified dominant species and intermediates in the reaction network, notably CHCH and H as the main species, and provided estimates for reaction barriers using a linear relationship between initial and transition states.
- Findings demonstrated that adding coinage metals to palladium decreases activity but increases selectivity by making the transformation easier (lowering barriers), highlighting the potential for further research in this area for different metal systems.
Article Synopsis
- The p85α subunit of PI3K influences cancer cell growth and mobility, contributing to their resistance to chemotherapy; TSWU-CD4 is a new compound that reduces cancer cell invasion by lowering MMP-2 expression.
- TSWU-CD4's impact on MMP-2 and invasion can be reversed by introducing the p85α subunit or specific active forms of MAPK, indicating that p85α and MAPK pathways are crucial for cancer cell motility.
- The mechanism involves disrupting the interaction between p85α and Rac1 in lipid rafts, suppressing MMP-2 through the PI3K-Akt-ERK-MAP kinase-NF-κB signaling pathway,
Article Synopsis
- HCIC is a novel technology for antibody separation using a resin called MMI-B-XL, which features a matrix of dextran-grafted agarose gel and 2-mercapto-1-methyl-imidazole as the functional ligand.
- The optimized resin can achieve a high ligand density of 200 μmol/g gel, significantly boosting its capacity for saturated adsorption and mass transport, especially when tested against the traditional MMI-B-6FF resin.
- Breakthrough experiments demonstrate that this new resin supports higher superficial velocities and dynamic adsorption, indicating its potential for large-scale antibody purification.
Aim: Androgen receptor (AR) antagonists have proven to be useful in the early control of prostate cancer. The aim of this study was to identify and characterize a novel β-amino-carbonyl-based androgen receptor antagonist.
Methods: Different isomers of the β-amino-carbonyl compounds were obtained by chiral separation.
Evaluation of mixed-mode chromatographic resins for separating IgG from serum albumin containing feedstock.
J Chromatogr B Analyt Technol Biomed Life Sci
October 2013
Mixed-mode chromatography has been focused as a cost-effective new technique for antibody purification. In this study, four mixed-mode resins with N-benzyl-N-methyl ethanol amine, 2-benzamido-4-mercaptobutanoic acide, 4-mercapto-ethyl-pyridine and phenylpropylamine as the ligands were tested and the multi-functional interactions between ligand and protein were discussed. Immunoglobulin G (IgG), bovine serum albumin (BSA) and the binary mixture of BSA and IgG were used as the model feedstock to compare the separation behaviors by pH gradient elution.
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- HCIC is a new method for purifying antibodies that depends on how ligands and pore sizes in the resin affect protein separation.
- New ligands (MMI) were attached to different agarose matrices to test their variation in ligand density and pore size in order to analyze the adsorption properties using bovine serum immunoglobulin as a model.
- The study found that increasing ligand density and pore size generally enhanced protein adsorption, while the addition of salt impacted adsorption differently based on the ligand density, suggesting optimization is needed for effective antibody purification.
Article Synopsis
- Immunoglobulin G (IgG) is a key plasma protein used in various therapeutic and diagnostic applications, which can be purified via ion exchange chromatography.
- The study uses bovine IgG to explore how different ligand densities and pore sizes of ion-exchange resins affect protein adsorption behaviors, employing Langmuir equations and pore diffusion models for data analysis.
- Findings reveal that higher ligand densities and smaller pore sizes enhance adsorption capacities, suggesting an optimal combination of these factors is crucial for effective protein purification.
Ion exchange chromatography (IEC) is a common and powerful technique for the purification of proteins. The ligand density and pore properties of ion-exchange resins have significant effects on the separation behaviors of protein, however, the understandings are quite limited. In the present work, the adsorption isotherms of bovine serum albumin (BSA) and human serum albumin (HSA) were investigated systematically with series of diethylaminoethyl (DEAE) ion-exchange resins, which have different ligand densities and pore sizes.
View Article and Find Full Text PDFExpression and purification of bioactive high-purity human midkine in Escherichia coli.
J Zhejiang Univ Sci B
February 2009
Article Synopsis
- Midkine is a growth factor that regulates cell growth and differentiation, and its non-tagged recombinant form is essential for functional studies.
- rhMK was successfully expressed in E. coli and its production was enhanced using IPTG, followed by a series of purification steps including sonication and ion-exchange chromatography.
- The final product of rhMK was over 98% pure and was shown to stimulate the growth of NIH3T3 cells.
Rhodanine derivatives as novel peroxisome proliferator-activated receptor gamma agonists.
Acta Pharmacol Sin
December 2007
Aim: To characterize the in vitro bioactivities of rhodanine derivatives as novel peroxisome proliferator-activated receptor (PPAR) gamma modulators, based on a hit (SH00012671) identified during high-throughput screening (HTS) of a diverse synthetic compound library, and to preliminarily elucidate the structure-activity relationship of this class of PPARgamma agonists.
Methods: Full-length PPARgamma and retinoid X receptor alpha (RXRalpha), biotinylated PPAR response element (PPRE), [3H]BRL49653 (rosiglitazone), and streptavidin-coated FlashPlate or microbeads were used to measure the receptor-binding properties of various compounds based on the scintillation proximity assay (SPA) technology. A recombinant PPRE vector was transiently cotransfected with PPARgamma and RXRalpha plasmids into the African green monkey kidney (CV-1) cells, and the effects of BRL49653 and test compounds on transcription mediated by PPARgamma were determined by examining luciferase (reporter) responses.
Inhibitory effect of Ca2+ on in vivo gene transfer by electroporation.
Acta Pharmacol Sin
March 2006
Article Synopsis
- The study aimed to understand how calcium ions (Ca2+) affect gene expression during electroporation-mediated gene transfer in mice.
- Results showed that adding just 10 mmol/L Ca2+ significantly decreased transgene expression to less than 5% of normal levels, with no other ions having the same effect.
- The inhibitory impact of Ca2+ appears to be linked to changes in the cell membrane after electroporation, affecting how well the gene transfer works.
[Limitations tetanus: case report and causes for misdiagnosis].
Shanghai Kou Qiang Yi Xue
March 2002