Cancer cell membrane-cloaked mesoporous silica nanoparticles with a pH-sensitive gatekeeper for cancer treatment.

Nanoparticular drug delivery system (NDDS) has great potential for enhancing the efficacy of traditional chemotherapeutic drugs. However, it is still a great challenge to fabricate a biocompatible NDDS with simple structure capable of optimizing therapeutic efficacy, such as high tumor accumulation, suitable drug release profile (e.g.

[Construction of prokaryotic expression vector of the fusion gene IFN-alpha1b/CSP II and expression in E. coli].

Objective: To Construct the prokaryotic expression vector of the fusion gene IFN-alpha1b/CSP II.

Methods: IFN-alpha1b was amplified from the human genomic DNA by PCR and cloned into prokaryotic expression vector pGEX-4T-1. The recombinant plasmid pGEX-4T-1/IFN-alpha1b was constructed.

[Establishment of computer-based video-tracking conditioned place preference experiment system in mice].

Objective: To establish the computer-based video-tracking conditioned place preference (CPP) system in mice.

Methods: The CPP system was composed of computer, camera, soundproof box, shuttle box and analytical software. The results of morphine-induced conditioned place preference were used to evaluate the experiment system.