[Anatomic study and clinical application of thinned posterior tibial artery perforator flap].

Objective: To explore the feasibility and therapeutic effect of thinned posterior tibial artery free perforator flap for the reconstruction of soft tissue defects at dorsum of hands.

Methods: Six fresh adult lower limbs specimens were injected with red latex via arterial cannula and dissected. The number, distribution, branches, and outer diameter of posterior tibia artery perforators were observed.

[Construction and identification of small interfering RNA expression plasmid targeting Sox9 and the function to cell growth and apoptosis of human chondrosarcoma cells HTB94].

Objectives: To construct small interfering (siRNA) Sox9 expression plasmid and transfer it into human chondrosarcoma cells HTB-94, and to check the mRNA and protein expression of Sox9 and cell growth and apoptosis of HTB-94 human chondrosarcoma cells.

Methods: siRNA(Sox9) expression plasmid was designed and synthesized. And it was transferred into HTB-94 human chondrosarcoma cells.