miR-495-3p depresses cell proliferation and migration by downregulating HMGB1 in colorectal cancer.

Background: MicroRNAs play an important role in the genesis and progression of tumours, including colorectal cancer (CRC), which has a high morbidity and mortality rate. In this research, the role of miR-495-3p and HMGB1 in CRC was investigated.

Methods: We performed qRT-PCR to detect the expression of miR-495-3p in colorectal cancer tissues and cell lines.

Research progress review on long non-coding RNA in colorectal cancer.

Colorectal cancer (CRC) is one of the most common malignancies globally, and the morbidity and mortality rates associated with it are among the highest around the world. Not even great advances in colorectal cancer diagnosis and treatment technologies have been able to increase the 5-year survival rate in this disease. Recidivation and metastasis are the main causes of death in CRC, although the underlying mechanism remains unknown.

Comparative diagnostic accuracy of ACE-III and MoCA for detecting mild cognitive impairment.

Objective: The aim of this study was to validate the reliability of the Chinese version of Addenbrooke's Cognitive Examination III (ACE-III) for detecting mild cognitive impairment. Furthermore, the present study compares the diagnostic accuracy of ACE-III with that of Montreal Cognitive Assessment (MoCA).

Methods: One hundred and twenty patients with MCI and 136 healthy controls were included in the study.

Hydrogen sulfide inhibits the renal fibrosis of obstructive nephropathy.

Hydrogen sulfide has recently been found decreased in chronic kidney disease. Here we determined the effect and underlying mechanisms of hydrogen sulfide on a rat model of unilateral ureteral obstruction. Compared with normal rats, obstructive injury decreased the plasma hydrogen sulfide level.

Autophagic impairment contributes to systemic inflammation-induced dopaminergic neuron loss in the midbrain.

Background: Neuroinflammation plays an important role in the pathogenesis of Parkinson's disease (PD), inducing and accelerating dopaminergic (DA) neuron loss. Autophagy, a critical mechanism for clearing misfolded or aggregated proteins such as α-synuclein (α-SYN), may affect DA neuron survival in the midbrain. However, whether autophagy contributes to neuroinflammation-induced toxicity in DA neurons remains unknown.

Crosstalk between the proteasome system and autophagy in the clearance of α-synuclein.

Aim: A growing body of evidence suggests that α-synuclein accumulation may play an important role in the pathogenesis of Parkinson's disease. The aim of this study was to investigate the roles of the proteasome and autophagy pathways in the clearance of wild-type and mutant α-synuclein in PC12 cells.

Methods: PC12 cells overexpressing either wild-type or A30P mutant α-synuclein were treated with the proteasome inhibitor epoxomicin, the macroautophagy inhibitor 3-MA and the macroautophagy activator rapamycin alone or in combination.

Application and interpretation of current autophagy inhibitors and activators.

Autophagy is the major intracellular degradation system, by which cytoplasmic materials are delivered to and degraded in the lysosome. As a quality control mechanism for cytoplasmic proteins and organelles, autophagy plays important roles in a variety of human diseases, including neurodegenerative diseases, cancer, cardiovascular disease, diabetes and infectious and inflammatory diseases. The discovery of ATG genes and the dissection of the signaling pathways involved in regulating autophagy have greatly enriched our knowledge on the occurrence and development of this lysosomal degradation pathway.

dl-3-n-Butylphthalide prevents oxidative damage and reduces mitochondrial dysfunction in an MPP(+)-induced cellular model of Parkinson's disease.

The aim of the present study was to explore the neuroprotective effects and mechanisms of action of dl-3-n-butylphthalide (NBP) in a 1-methyl-4-phenylpyridiniumion (MPP(+))-induced cellular model of Parkinson's disease (PD). NBP was extracted from seeds of Apium graveolens Linn. (Chinese celery).

[PCR-based STR genotyping of sweat latent fingerprint samples involved in cases].

Objective: To study the extracting and genotyping method of sweat latent fingerprint samples involved in cases.

Method: Chlex 100 extraction method was used to extract DNA. STR loci were typed after PCR amplification by Profiler Plus kit.