Publications by authors named "Hui Xia Zheng"
Aims: To investigate the expression of epithelial cell transforming sequence 2 (ECT2) in invasive breast cancer and its prognostic significance.
Methods: ECT2 immunohistochemical detection was performed in 165 breast cancer specimens and 100 normal control tissues. Univariable and multivariable Cox proportional hazards regression model analysis was used to confirm independent prognostic factors.
Inhibitory effects of dobutamine on human gastric adenocarcinoma.
World J Gastroenterol
December 2014
Aim: To explore the inhibitory effects of dobutamine on gastric adenocarcinoma cells.
Methods: Dobutamine was used to treat gastric adenocarcinoma cells (SGC-7901) and cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of dobutamine combined with cisplatin on cell viability were also analyzed.
[Comparison of mannitol and hypertonic saline in treatment of intracranial hypertension of rabbits].
Zhejiang Da Xue Xue Bao Yi Xue Ban
March 2012
Objective: To compare the effects of mannitol and hypertonic saline (HS) in treatment of intracranial hypertension (ICH) of rabbits.
Methods: The animal mode of ICH was established by perfusing artificial cerebrospinal fluids (aCSF) with controlled pressure into the cerebral ventricles of rabbits. The mean arterial pressure, respiratory rate, tidal volume, perfusion rate of aCSF and water content of cerebrum were investigated in rabbits with ICH after a single bolus of 20% mannitol (5 ml/kg), 7.
[Therapeutic effect of in vitro 5-aza-2'-deoxycytidine combined with imatinib on gastrointestinal stromal tumor].
Zhonghua Wei Chang Wai Ke Za Zhi
March 2012
Objective: To investigate the impact of 5-aza-2'-deoxycytidine(5-aza-CdR) combined with imatinib on the proliferation, motility, invasion, and apoptosis of gastrointestinal stromal tumors(GIST) cells in vitro.
Methods: MTT assay was used to investigate the effect of the two agents on proliferation of GIST882. Plate colony forming assay was used to determine the number of colony-forming.
Aims: To detect microsatellite loci alterations by fluorescent multiplex PCR in urine sediment cell of urothelial carcinoma, and to determine if they can be used as genetic markers for diagnosis of urothelial carcinoma.
Materials And Methods: Microsatellite alteration analysis was conducted using fluorescent multiplex PCR with samples from 64 cases of urothelial carcinomas of the bladder. Three microsatellites spanning the 3p14 and two additional microsatellites in 9q33 and 9p22 were analyzed.
Article Synopsis
- SPARC is linked to the progression of colon cancer and plays a role in tumor invasion and angiogenesis, with its expression primarily found in the stromal cells around tumors.
- In a study involving 114 patients, researchers assessed the relationship between SPARC and VEGF (vascular endothelial growth factor) using tissue samples from cancerous and non-diseased colon, finding that SPARC levels were significantly correlated with VEGF expression and microvessel density.
- Low or absent levels of SPARC in tissues were associated with poorer overall and disease-free survival rates, suggesting that SPARC could be a valuable prognostic marker and potential target for colon cancer treatment.
The purpose of this study was to detect the protein and mRNA expression of dysadherin and to investigate the clinical significance of dysadherin expression in gastrointestinal stromal tumors (GISTs). Twenty fresh GISTs samples were used for testing the mRNA of dysadherin and E-cadherin by reverse-transcription polymerase chain reaction (RT-PCR). In addition, 54 paraffin blocks of GISTs cases were also used for the investigation of dysadherin and E-cadherin using immunohistochemistry.
View Article and Find Full Text PDF[Prognostic value of P16 gene methylation and P16 protein expression in gastrointestinal stromal tumor].
Zhonghua Wei Chang Wai Ke Za Zhi
July 2007
Objective: To study the methylation status of P16 gene promoter and the expression of P16 protein in gastrointestinal stromal tumors(GIST) and to explore the prognostic value.
Methods: Methylation status of the P16 promoter was detected by methylation- specific polymerase chain reaction (MSP) and the expression of P16 protein by immunohistochemistry in 62 patients with GIST.
Results: The status of P16 gene methylation and the expression of P16 protein were significantly different among the patients with different subclassification of GIST using Fletcher's scheme (P < 0.