In this study, we investigated the changes in untargeted metabolites using UHPLC-MS/MS and the flavors of nonflavored (BS1) and flavored (BS2) roasted beef using GC-MS throughout a 6-month frozen period. A total of 509, 659, and 496 metabolites met the conditions for differential screening, and 56, 103, and 47 differential metabolites were recognized between BS1 and BS2 at 0, 3, and 6 months of frozen periods, respectively. The total relative abundance of organic nitrogen compounds, phenylpropanoids, polyketides, organic acids and their derivatives, and benzenoids increased during frozen storage at 3 months and then decreased at 6 months.
View Article and Find Full Text PDFObjectives: Triglyceride-glucose (TyG) index, which is a valuable measure of insulin resistance, has been found to have predictive value for cardiovascular disease (CVD). However, its relationship with CVD among individuals with chronic kidney disease (CKD) has not been thoroughly investigated. This study focused on examining the relationship of the TyG index and CVD among CKD patients in United States.
View Article and Find Full Text PDFEthnopharmacological Relevance: Huangkui capsule (HKC), a patent traditional Chinese medicine, has shown significant efficacy in managing chronic kidney disease (CKD), particularly diabetic nephropathy (DN). Previous studies have shown that HKC can alleviate kidney damage in DN. However, the exact mechanisms through which it exerts its effects remain unclear.
View Article and Find Full Text PDFNanobowls show promising potential in biomedical applications, such as bioimaging, cargo delivery, and disease theranostics, due to their unique concave structure and interior cavities. However, the lack of biodegradable nanobowls with manipulable size (especially the dent size) still exists as an obstacle for their in-depth exploration and application in biomedical fields. Herein, polypeptide-based nanobowls are successfully obtained by the self-assembly of a graft polypeptide [named TPE-P(GAAzo--GA)] via a solvent-switch method.
View Article and Find Full Text PDFPolyzwitterions refer to polymers containing both positive and negative charged groups in one side chain, which have shown unique physicochemical properties and significant potential in diverse applications due to their amphiphilic and net-neutral charged properties. This review aims to highlight the recent advances in the design and synthesis of polyzwitterions including direct polymerization of zwitterionic monomers and deionization of polymers. Furthermore, the formation of polyzwitterion based soft materials such as nanoparticles by self-assembly, hydrogels, coatings and polyzwitterion brushes, as well as the influence of the microstructure on their properties and applications are discussed.
View Article and Find Full Text PDFEffective hydrolysis of lignocelluloses for producing reducing sugar is impeded by the covalent binding of hemicellulose and cellulose through lignin, which could be eliminated by laccases. This study identified a novel thermostable laccase from Bacillus safensis TCCC 111022 and created an iterative mutant E231D/Y441H, exhibiting 1.59-fold greater specific activity and a 183 % greater half-life at 80°C than the wild-type enzyme.
View Article and Find Full Text PDFImmunotherapy has revolutionized cancer treatment, yet its effectiveness is limited by immunosuppressive tumor microenvironment (TME). To overcome this challenge, innovative strategies to effectively modulate the TME are urgently needed. Over the past decades, bacteria-mediated cancer immunotherapy has recaptured increasing attention, driven by advances in synthetic biology, genetic engineering and our knowledge of host-pathogen interactions.
View Article and Find Full Text PDFBackground: Obesity increases the risk of papillary thyroid carcinoma (PTC) and lymph node metastasis (LNM), possibly via modulation of the tumor immunological microenvironment.
Materials And Methods: The STROCSS guideline was followed to conduct a retrospective cohort study. Binary logistic regression analysis with odds ratios (OR) was performed to assess the association between tumor-infiltrating lymphocytes (TILs), obesity, and LNM.
Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality. LncRNA NORAD is frequently upregulated and positively associated with various cancer progressions. We discovered NORAD was significantly upregulated in CRC tissues and cells.
View Article and Find Full Text PDFThe spectrum of synucleinopathies, including Parkinson's disease (PD), multiple system atrophy (MSA), and dementia with Lewy bodies (DLB), is characterized by α-synuclein (αSyn) pathology, which serves as the definitive diagnostic marker. However, current diagnostic methods primarily rely on motor symptoms that manifest years after the initial neuropathological changes, thereby delaying potential treatment. The symptomatic overlap between PD and MSA further complicates the diagnosis, highlighting the need for precise and differential diagnostic methods for these overlapping neurodegenerative diseases.
View Article and Find Full Text PDFJMIR Form Res
November 2024
Ecotoxicol Environ Saf
November 2024