Publications by authors named "Hui Dong Zhang"
Article Synopsis
- The structure of mature forests is shaped by long-term ecological processes, leading to diverse plant species and vital ecological functions.
- A study of Mongolian oak forests in eastern Liaoning discovered specific patterns in tree size and distribution, highlighting dominant species across different growth layers.
- Findings indicated that competition among trees influences their growth and arrangement, with larger trees facing less competition, while smaller ones are more affected by both intraspecies and interspecies rivalry.
Article Synopsis
- The separation of CF from CF/N mixtures is essential for improving efficiency in the electronics industry due to CF's role as an etching gas.
- High-throughput computational techniques and GCMC simulations were used to assess 247 pure silicon zeolite materials for their effectiveness in adsorbing and separating CF from N, focusing on metrics like adsorption selectivity and regenerability.
- The study identified the top three zeolite structures for both vacuum and pressure swing adsorption processes, aiming to guide future experimental efforts in CF/N adsorption and separation.
Galactinol Regulates JA Biosynthesis to Enhance Tomato Cold Tolerance.
J Agric Food Chem
February 2024
Article Synopsis
- Low temperatures negatively affect plant growth and fruit yield; this study shows that enhancing galactinol synthase expression can improve tomato cold tolerance.
- Increased levels of jasmonic acid (JA) and certain proteins were observed in genetically modified plants, indicating a complex relationship between galactinol and various signaling pathways.
- The findings suggest that galactinol plays a significant role in regulating JA biosynthesis and enhancing cold tolerance through specific gene interactions and regulatory mechanisms.
Article Synopsis
- The study analyzed how different stand densities and climate factors influence the radial growth of trees in a secondary forest using tree ring data.
- Results indicated that lower stand density led to greater average radial growth (3.12 mm) compared to medium and high densities (1.55, 1.42, and 0.96 mm, respectively).
- Thinning was found to positively impact medium-density forests, while the negative effects of climate variability, particularly precipitation in January and February, were reduced by thinning, suggesting it could help counteract climate change impacts.
Article Synopsis
- The hexametric T7 helicase (gp4) wraps around a DNA strand and works to unwind it, but its exact mechanism for doing this is still not fully understood.* -
- Researchers used advanced techniques to study how gp4 interacts with DNA, especially when there are abasic lesions (missing nucleotides), and found that these lesions slow down but don't completely stop the unwinding process.* -
- The study revealed that gp4 moves back and forth more when encountering lesions and steps forward by one to four nucleotides on normal DNA, suggesting that a specific intermediate state during unwinding might help gp4 navigate around these lesions.*
Article Synopsis
- Soluble carbohydrates like raffinose family oligosaccharides (RFOs) play a crucial role in helping plants tolerate cold stress and influencing their growth.
- Researchers focused on galactinol, vital for RFO production, to see if it acts as a signaling molecule for cold tolerance.
- In their study, they discovered that expressing galactinol synthase (AnGolS1) from a desert plant in tomatoes not only improved cold tolerance but also altered sugar profiles and enhanced ethylene signaling during seed germination.
Article Synopsis
- Maintaining forest structural diversity helps ensure forest stability and biodiversity, particularly in a primary mixed broadleaved Korean pine forest studied in eastern Liaoning.
- The study found that the diameter distribution of trees followed an inverse J-shape, signaling good understory regeneration alongside larger trees.
- Main trees were randomly distributed, with an optimal mix allowing for resource use and supporting both small-diameter and dominant canopy trees in the community.
Article Synopsis
- The CRNDE gene, a long noncoding RNA, is found to be increased in gastric cancer and may play a role in regulating cell growth.
- Research methods included assessing expression levels through qRT-PCR and examining cell viability with MTT assays, revealing that higher CRNDE levels correlated with increased growth and colony formation of gastric cancer cells.
- The study highlights CRNDE's interaction with the miR-145 and its target E2F3, suggesting that CRNDE could be a potential target for gastric cancer therapies.
Article Synopsis
- A miniature pig model was created to study ischemic mitral regurgitation (IMR) by inducing ischemia through posterior mitral chordae tendinae rupture and placing an ameroid constrictor around the left circumflex coronary artery.
- Evaluation methods including X-ray coronary angiography, ECG, echocardiography, and MRI were employed over time to analyze heart structure and function, revealing significant pathological changes and increases in both left atrial and left ventricular dimensions post-operation.
- The developed pig model successfully mimics the progression of IMR in humans, showing features such as scar tissue, inflammatory cell infiltration, and increased heart chamber volumes, which may aid in future studies of this condition.
JMJD2A predicts prognosis and regulates cell growth in human gastric cancer.
Biochem Biophys Res Commun
June 2014
A number of JmjC domain-containing histone demethylases have been identified and biochemically characterized in mammalian. JMJD2A is a transcriptional cofactor and enzyme that catalyzes demethylation of histone H3 lysines 9 and 36. Here in this study, we aim to explore the role of JMJD2A in human gastric cancer.
View Article and Find Full Text PDFThe RNA-binding protein PCBP2 facilitates gastric carcinoma growth by targeting miR-34a.
Biochem Biophys Res Commun
June 2014
Article Synopsis
- Gastric carcinoma is the fourth most prevalent cancer globally, often resulting in high mortality and low survival rates; however, its underlying mechanisms remain unclear.
- The study highlights RNA-binding protein PCBP2 as a key oncogenic factor in gastric cancer, found to be elevated in cancer tissues and linked to poorer survival outcomes.
- Investigating further, the research indicates that suppressing PCBP2 reduces cancer cell growth and promotes cell death by influencing the balance of pro-apoptotic and anti-apoptotic proteins through the regulation of miR-34a.
Objective: To evaluate the therapeutic effect and security of CT guided unilateral percutaneous vertebroplasty (PVP) for the treatment of osteoporotic vertebral compression fracture (OVCF) in senile patients.
Methods: From April 2009 to June 2010, 26 patients undergoing CT guided unilateral percutaneous vertebroplasty were analyzed retrospectively. There were 9 males and 17 females,ranging in age from 60 to 85 years with an average of (67.
The olfactory sense plays a key role in animals'life time. The main gene related with olfaction was olfactory receptor (OR) gene. This review introduced the structure, expression regulation, distribution, molecular evolution and polymorphism of OR gene.
View Article and Find Full Text PDFA total of 1139 trees from 8 clear-cut stands dominated by fir, spruce, and pine in the Changbai Mountains were selected to study the relationships between the coefficient of variation of diameter and height and the competition index of the three main coniferous tree species in the Mountains. For the test tree species, the variation of height vs. diameter class was relatively small, while the variations of diameter and height vs.
View Article and Find Full Text PDFArticle Synopsis
- The gene Rv0901 from Mycobacterium tuberculosis is not fully understood, prompting research to amplify and express this protein for study.
- Researchers created a recombinant plasmid using Rv0901 and tested its effects on mammalian cells and animals, measuring various immune responses and survival rates.
- Findings indicate that Rv0901 may play a role in the virulence and immunogenic properties of M. tuberculosis.
Objective: To study the function of Loa22 gene from virulent serovar Lai. L. interrogans by expressing its protein.
View Article and Find Full Text PDF[Recombinant plasmid constructed and cytotoxicity studied for outer membrane protein LipL32 gene of Leptospira strain 017].
Sichuan Da Xue Xue Bao Yi Xue Ban
May 2008
Article Synopsis
- The study successfully created a recombinant plasmid that contains the LipL32 gene from Leptospira strain 017 and investigated the cytotoxic effects of the protein it produces.
- The LipL32 gene was amplified using PCR and synthesized in E. coli, where it formed inclusion bodies consisting of the target protein.
- The expressed protein was shown to be immunogenic and significantly increased the release of toxicity markers (LDH and NO) in ECV304 cells compared to controls, indicating its cytotoxic nature.
[Immunogenicity of DNA vaccine prime-BCG boost against Mycobacterium tuberculosis].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
February 2008
Aim: To compare the immunogenicity of vaccine strategies about combined DNA prime-BCG boost and DNA or BCG vaccination only.
Methods: BALB/c mice were divided into four groups each containing 6 mice, and all mice received three immunizations at 2 weeks interval. One group of mice was vaccinated with PBS (PBS group); one group was vaccinated twice at 0 week and 2 weeks with combined DNA expressing two mycobacterial antigens, ESAT-6 and antigen 85A respectively; and then boosted with BCG at 4 weeks (DNA/BCG group); another group was vaccinated twice with combined DNA at 0 week, 2 weeks and 4 weeks(DNA/DNA group); and the final group received PBS twice at 0 week and 2 weeks and then vaccinated with BCG at 4 weeks(BCG group).
[Construction of the eukaryotic recombinant vector and expression of the outer membrane protein LipL32 gene from Leptospira serovar Lai].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
February 2008
Aim: To construct the eukaryotic experssion vector of LipL32 gene from Leptospira serovar Lai and express the recombinant plasmid in COS-7 cell.
Methods: The LipL32 gene was amplified from Leptospira strain 017 genomic DNA by PCR and cloned into pcDNA3.1, through restriction nuclease enzyme digestion.
[Protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis].
Sichuan Da Xue Xue Bao Yi Xue Ban
March 2007
Article Synopsis
- The study aimed to explore the interaction between two hypothetical proteins, Rv1246c and Rv1247c, in Mycobacterium tuberculosis.
- Using PCR, the full gene sequences of Rv1246c and Rv1247c were amplified and cloned into specific vectors to create recombinant plasmids for yeast transformation.
- The successful growth of yeast cells on selective plates and positive beta-galactosidase activity confirmed that Rv1246c and Rv1247c interact with each other in yeast.
[Construction and screen of recombinant BCG strain expressing and secreting human interleukin 12 protein].
Sichuan Da Xue Xue Bao Yi Xue Ban
March 2007
Objective: To screen and construct the recombinant Bacillus Calmette-Guerin (rBCG) strain expressing and secreting the human interleukin 12 (rBCG-12).
Methods: IL-12 complete gene including p40 and p35 subunits was amplified by PCR from a plasmid pORF-h IL-12 and cloned into E. coli-Mycobacteria shuttle vector pMV361.
Article Synopsis
- The study aimed to assess how Tongxinluo and Simvastatin stabilize plaques in aortic atherosclerosis and investigate the underlying molecular mechanisms.
- Twenty-three rabbits were subjected to aortic balloon injury and high-cholesterol diet, then assigned to receive either Tongxinluo, Simvastatin, or no treatment for 16 weeks, while a control group was also included.
- Results showed that while Simvastatin significantly lowered cholesterol levels compared to the model group, Tongxinluo did not have a notable effect on blood lipid levels, and significant changes in endothelial function markers were noted across the groups.
[Construction of recombinant E. coli-L. interrogans shuttle plasmid pGKINVA and screening recombinant leptospira strains].
Sichuan Da Xue Xue Bao Yi Xue Ban
January 2007
Article Synopsis
- The study aimed to explore the role of the invA gene in the pathogenicity of Leptospira by creating a recombinant vector and introducing it into L. biflexa.
- The invA gene was successfully cloned into a shuttle vector, and after electroporation into the L. biflexa strain Patoc I, suitable mutants were identified through PCR.
- The findings suggest that the generated recombinant L. biflexa strain can serve as a model for further research into the pathogenic functions of invA in living organisms.
[Molecular cloning and expression of hypothetical proteins Rv1494 and Rv1495 of M.tuberculosis H37Rv strain].
Nan Fang Yi Ke Da Xue Xue Bao
January 2007
Objective: The mechanism by which M.tuberculosis persists and survives in host macrophage is not fully understood, however, the M. tuberculosis chromosome-encoded TA loci perform functions possibly of signaling to these processes.
View Article and Find Full Text PDF[Construction and expression of the eukaryotic plasmids containing different subunits of human IL-12 gene].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
November 2006
Aim: To clone and construct the eukaryotic expression plasmids containing different subunits of human IL-12 and to investigate its expression following transient transfection into COS-7 cells.
Methods: Human leukaemia cells (HL-60 and THP-1) were stimulated by DMSO, IFN-gamma and LPS, and the target genes including IL-12 p40 and p35 subunits were amplified by RT-PCR. P40 and p35 fragments were then connected and the recombinant plasmids pcDNA-p40, pcDNA-p35 and pcDNA-p40-p35 were constructed by double restriction enzymes digestion and connection.