Publications by authors named "Hugh Boothe"
Objectives: We evaluated Nanopore sequencing for influenza surveillance.
Methods: Influenza A and B PCR-positive samples from hospital patients in Oxfordshire, UK, and a UK-wide population survey from winter 2022-23 underwent Nanopore sequencing following targeted rt-PCR amplification.
Results: From 941 infections, successful sequencing was achieved in 292/388 (75 %) available Oxfordshire samples: 231 (79 %) A/H3N2, 53 (18 %) A/H1N1, and 8 (3 %) B/Victoria and in 53/113 (47 %) UK-wide samples.
Article Synopsis
- Syndromic surveillance traditionally depends on healthcare-seeking patients, but this study suggests that involving community cohorts can yield valuable insights, especially given the presence of multiple respiratory viruses during the 2022/23 winter season in the UK.
- The research analyzed data from nearly 33,000 tests, revealing SARS-CoV-2, RSV, and influenza A/B positivity rates, with peaks of these viruses occurring at different times and varying by age group, highlighting that younger individuals were most affected by RSV and older individuals by SARS-CoV-2.
- Findings indicated that many individuals who tested positive reported few symptoms, complicating the ability to distinguish between the viruses solely based on symptoms, and
Intracolonic vancomycin for severe Clostridium difficile colitis.
Surg Infect (Larchmt)
December 2013
Background: Clostridium difficile colitis is associated with increased age, antibiotic usage, and hospitalization. Severe C. difficile colitis refractory to medical therapy may require surgical intervention including subtotal colectomy.
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