Machine Learning Models for Segmentation and Classification of Cyanobacterial Cells.

Timelapse microscopy has recently been employed to study the metabolism and physiology of cyanobacteria at the single-cell level. However, the identification of individual cells in brightfield images remains a significant challenge. Traditional intensity-based segmentation algorithms perform poorly when identifying individual cells in dense colonies due to a lack of contrast between neighboring cells.

Role of carboxysomes in cyanobacterial CO assimilation: CO concentrating mechanisms and metabolon implications.

Many carbon-fixing organisms have evolved CO concentrating mechanisms (CCMs) to enhance the delivery of CO to RuBisCO, while minimizing reactions with the competitive inhibitor, molecular O . These distinct types of CCMs have been extensively studied using genetics, biochemistry, cell imaging, mass spectrometry, and metabolic flux analysis. Highlighted in this paper, the cyanobacterial CCM features a bacterial microcompartment (BMC) called 'carboxysome' in which RuBisCO is co-encapsulated with the enzyme carbonic anhydrase (CA) within a semi-permeable protein shell.

Computational modeling and evolutionary implications of biochemical reactions in bacterial microcompartments.

Bacterial microcompartments (BMCs) are protein-encapsulated compartments found across at least 23 bacterial phyla. BMCs contain a variety of metabolic processes that share the commonality of toxic or volatile intermediates, oxygen-sensitive enzymes and cofactors, or increased substrate concentration for magnified reaction rates. These compartmentalized reactions have been computationally modeled to explore the encapsulated dynamics, ask evolutionary-based questions, and develop a more systematic understanding required for the engineering of novel BMCs.

Short chain α-pyrones capable of potentiating penicillin G against Pseudomonas aeruginosa.

The dramatic increase in bacterial resistance over the past three decades has greatly reduced the effectiveness of nearly all clinical antibiotics, bringing infectious disease to the forefront as a dire threat to global health. To combat these infections, adjuvant therapies have emerged as a way to reactivate known antibiotics against resistant pathogens. Herein, we report the evaluation of simplified α-pyrone adjuvants capable of potentiating penicillin G against Pseudomonas aeruginosa, a Gram-negative pathogen whose multidrug-resistant strains have been labeled by the Centers for Disease Control and Prevention as a serious threat to public health.

Development of a Robust and Quantitative High-Throughput Screening Method for Antibiotic Production in Bacterial Libraries.

Over the past 30 years, there has been a dramatic rise in the number of infections caused by multidrug-resistant bacteria, which have proliferated due to the misuse and overuse of antibiotics. Over this same time period, however, there has also been a decline in the number of antibiotics with novel mechanisms of action coming to market. Therefore, there is a growing need for an increase in the speed at which new antibiotics are discovered and developed.

Ethics in contemporary community psychiatry.

Community psychiatry is a wonderful and rewarding career path that increasing numbers of psychiatrists are choosing to practice.14 This article was created to provide an orientation to the characteristics of contemporary community psychiatric practice that render it distinct in terms of ethical considerations. We defined and described community practice.

Unlicensed residential programs: the next challenge in protecting youth.

Over the past decade in the United States, the number of private residential facilities for youth has grown exponentially, and many are neither licensed as mental health programs by states, nor accredited by respected national accrediting organizations. The Alliance for the Safe, Therapeutic and Appropriate use of Residential Treatment (A START) is a multi-disciplinary group of mental health professionals and advocates that formed in response to rising concerns about reports from youth, families and journalists describing mistreatment in a number of the unregulated programs. This article summarizes the information gathered by A START regarding unregulated facilities.

Snake oil, ethics, and the first amendment: what's a profession to do?

This article considers the appropriate legal and ethical response to those whose advocacy of "alternative" or unvalidated therapies places people at risk of harm. What are our professional responsibilities with respect to such advocacy, and what sorts of harm will justify government intervention?

The future of community psychiatry.

Leaders of national groups that have focused on issues of community and social psychiatry present their ideas about the future of psychiatry. They identify five areas: theory development; the relevance of community psychiatry in the 21st century; education and training; the relationship between community psychiatry and health maintenance organizations; and role of community psychiatry in bridging medical science with humanism. The unifying theme for these topics is that community psychiatry can be a vehicle for modifying general psychiatry's propensity towards individualism and reductionism by offering a more holistic and integrative approach to illness and well-being.

DC-STAMP, a novel multimembrane-spanning molecule preferentially expressed by dendritic cells.

Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification of a novel transcript, isolated by random sequencing of a cDNA library prepared from monocyte-derived DC, which we termed DC-specific transmembrane protein (DC-STAMP).

Development and Psychopathology: Fetal Alcohol Syndrome and Related Conditions.

Prenatal exposure to alcohol, which is a neurobehavioral teratogen, can cause defects in the structure and function of the developing central nervous system and can also impact growth and morphology. These wide-ranging effects occur along a continuum, with the most severe impact known as fetal alcohol syndrome (FAS). As the identified population of alcohol-affected patients grows, because of enhanced societal awareness and increased diagnostic capability, it has become clear that the continuum of fetal alcohol effects can mean lifelong disabilities with serious implications for function in adolescence and adulthood.

Psychoactive drugs, alcohol, and stress and coping processes in older adults.

Data are presented on a) the relationship between psychoactive substance use and personal characteristics, b) the relationship between psychoactive substance use and appraisal of and coping with stressful situations, and c) the use of psychoactive substances specifically to cope with stress among older people. Data were provided by 141 65-to-74-year-old retired Caucasians who were assessed repeatedly over 6 months. Compared to national samples, subjects were relatively heavy users of alcohol and average users of psychoactive drugs.

Assessing comprehension in a survey of public reactions to complex issues.

This paper reports results of research to develop household interview procedures for obtaining reasonably informed public judgements about ethical issues in biomedical research. We describe (1) an interviewing strategy designed to enhance comprehension among survey respondents, and (2) a composite comprehension index based on three types of information from the interview. Using data from a cross-section sample, we identify subgroups that differ significantly with respect to scores on the comprehension index, and we then describe the observed levels of comprehension in terms of the operations defining the index.

Madness and work: short- and long-term effects of mental illness on occupational careers.

This study assesses the impact of mental illness on occupational careers. Thirty-six married men who first entered mental hospitals in the 1950s were followed up in 1972 and eighteen men who were first hospitalized in 1973-74 were interviewed. Their histories reveal the importance of the development of competence in the work role prior to the onset of mental illness.