Molecular characterization and functional analysis of genes mediating emamectin benzoate action to the pinewood nematode (Bursaphelenchus xylophilus).

Emamectin benzoate (EB) is a highly effective and low-toxicity pesticide for the control of Bursaphelenchus xylophilus. However, its action mechanism in B. xylophilus has not yet been verified.

The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in .

is a dangerous quarantine pest that causes extensive damage to pine ecosystems worldwide. Cyclobutrifluram, a succinate dehydrogenase inhibitor (SDHI), is a novel nematicide introduced by Syngenta in 2013. However, the nematocidal effect of cyclobutrifluram against plant-parasitic nematodes remains underexplored.

Current advances in the identification of plant nematode diseases: From lab assays to in-field diagnostics.

Plant parasitic nematodes (PPNs) cause an important class of diseases that occur in almost all types of crops, seriously affecting yield and quality and causing great economic losses. Accurate and rapid diagnosis of nematodes is the basis for their control. PPNs often have interspecific overlays and large intraspecific variations in morphology, therefore identification is difficult based on morphological characters alone.

Population Structure in China Suggests a South-to-North Expansion.

The distribution range of root-knot nematode is rapidly expanding, posing a severe threat to rice production. In this study, the sequences of cytochrome oxidase subunit I () genes of rice populations from all reported provinces in China were amplified and sequenced by PCR. The distribution pattern and phylogenetic tree showed that all 54 populations in China have distinct geographical distribution characteristics; specifically, cluster 1 (southern China), cluster 2 (central south and southwest China), and cluster 3 (central and eastern China).

Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a Technology for Rapid and Highly Sensitive Detection of and .

The cereal cyst nematodes and are recognized as cyst nematodes that infect cereal crops and cause severe economic losses worldwide. Rapid, visual detection of cyst nematodes is essential for more effective control of this pest. In this study, recombinase polymerase amplification (RPA) combined with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a (formerly known as cpf1) was developed for the rapid detection of and from infested field samples.

Origin and Phylogeography of Chinese Cereal Cyst Nematode Revealed by Mitochondrial COI Sequences.

, a globally distributed plant-parasitic nematode, is one of the most significant pests on cereal crops. In China, it is widely distributed in cereal-growing areas of 16 provinces and causes serious yield losses. In the present study, a total of 98 populations of were collected from major wheat-growing regions in China and six other countries.

Prevalence and Molecular Diversity of Plant-Parasitic Nematodes of Yam ( spp.) in China, with Focus on spp.

There is little information about nematode pests associated with yam in China. Between 2020 and 2021, surveys of yam fields were conducted to investigate the abundance and prevalence of plant-parasitic nematodes in major yam growing areas. A total of 110 bulk soil samples from the yam rhizosphere and 48 yam tubers were collected from seven counties in Jiangxi and Shandong provinces.

Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection.

Background: Mulberry (Morus alba L.) is an important sericulture crop; however, root-knot nematode infection seriously limits its production. Understanding the mechanism of interaction between mulberry and nematode is important for control of infection.

Genetic Diversity of the root-knot nematode in Mulberry Based on the Mitochondrial COI Gene.

This study explores the genetic diversity and structure of in mulberry in China. The COI mitochondrial gene (mtCOI) in populations in Guangdong, Guangxi, and Hunan Provinces was PCR-amplified, sequenced, and analyzed for genetic diversity. The total number of variations, haplotypes (Hap), the average number of nucleotide differences (k), haplotype diversity (H), and nucleotide diversity (π) of mtCOI were 25, 11, 4.

Characterization of root-knot nematodes infecting mulberry in Southern China.

China is one of the largest producers of mulberry in the world. With the development of the sericulture industry, several pests and diseases have occurred in rapid succession, chief among which is the root-knot nematode disease affecting mulberry. According to the China cocoon and silk exchange, cocoon prices have doubled since the beginning of 2009 and rose to 92,700 yuan ($135,770) per tonne in mid-April 2010.