Band 40/41 Surface Acoustic Wave Filters on 42°YX-Lithium Tantalate Substrate with Suppression of Transverse Leakage.

The transverse leakage of leaky surface acoustic waves (LSAWs) occurs on 42°YX-lithium tantalate substrates (42LT), which increases the insertion loss, narrows the bandwidth and flattens the roll-off of band 40/41 SAW filters and duplexers. In this work, LSAW characteristics with different metal materials and thicknesses are calculated by the finite element method (FEM), which determines the IDT material and thickness used for band 40/41 SAW filter design. To deeply understand transverse leakage and suppress it, the effects of different gap and dummy lengths on transverse leakage are simulated and discussed.

CircRNA-regulated immune responses of asian honey bee workers to microsporidian infection.

is a widespread fungal parasite for honey bees, causing bee nosemosis. Based on deep sequencing and bioinformatics, identification of circular RNAs (circRNAs) in workers' midguts and circRNA-regulated immune response of host to invasion were conducted in this current work, followed by molecular verification of back-splicing sites and expression trends of circRNAs. Here, 10185 and 7405 circRNAs were identified in the midguts of workers at 7 days (AcT1) and 10 days (AcT2) post inoculation days post-inoculation with .

Deciphering the CircRNA-Regulated Response of Western Honey Bee () Workers to Microsporidian Invasion.

is a widespread fungal parasite of adult honey bees that leads to a serious disease called nosemosis. Circular RNAs (circRNAs) are newly discovered non-coding RNAs (ncRNAs) that regulate biological processes such as immune defense and development. Here, 8199 and 8711 circRNAs were predicted from the midguts of workers at 7 d (Am7T) and 10 d (Am10T) after inoculation (dpi) with spores.

Reconstruction and functional annotation of Ascosphaera apis full-length transcriptome utilizing PacBio long reads combined with Illumina short reads.

Ascosphaera apis is a widespread fungal pathogen of honeybee larvae that results in chalkbrood disease, leading to heavy losses for the beekeeping industry in China and many other countries. This work was aimed at generating a full-length transcriptome of A. apis using PacBio single-molecule real-time (SMRT) sequencing.

Transcriptome data of control and infected larval guts.

is an obligate fungal pathogen of honeybee larvae that leads to chalkbrood, which causes heavy losses for the apiculture in China and many other countries. In this article, guts of 4-, 5-, 6-day-old larvae challenged by (AmT1, AmT2, AmT3) and normal 4-day-old larval guts (AmCK) were sequenced using next-generation sequencing technology. On average, 29,196,197, 28,690,943, 29,779,715 and 30,496,725 raw reads were yielded from these four groups; an average of 29,540,895 clean reads were obtained after quality control.

Systematic identification of circular RNAs and corresponding regulatory networks unveil their potential roles in the midguts of eastern honeybee workers.

Currently, knowledge of circular RNAs (circRNAs) in insects including honeybee is extremely limited. Here, differential expression profiles and regulatory networks of circRNAs in the midguts of Apis cerana cerana workers were comprehensively investigated using transcriptome sequencing and bioinformatics. In total, 9589 circRNAs (201-800 nt in length) were identified from 8-day-old and 11-day-old workers' midguts (Ac1 and Ac2); among them, 5916 (61.

MicroRNA dataset of normal and -infected midguts of workers.

is a widespread fungal pathogen of honeybees, which is infective to all castes in the colony, including queens, drones and workers. Nosemosis caused by poses a big challenge for apiculture all over the world. Here, midguts of normal and -infected workers at 7 and 10 days post infection were sequenced utilizing small RNA sequencing (sRNA-seq) technology.

A comprehensive transcriptome data of normal and -stressed midguts of workers.

Honeybees are pivotal pollinators of crops and wild flora, and of great importance in supporting critical ecosystem balance. , a unicellular fungal parasite that infects midgut epithelial cells of honeybees, can dramatically reduce honeybee population and productivity. Here, midguts of workers at 7 d and 10 d post inoculation (dpi) with sucrose solution (Ac7CK and Ac10CK) and midguts at 7 dpi and 10 dpi with sucrose solution containing spores (Ac7T and Ac10T) were sequenced using strand-specific cDNA library construction and next-generation sequencing.

Comparative Identification of MicroRNAs in Workers' Midguts in Responseto Invasion.

Here, the expression profiles and differentially expressed miRNAs (DEmiRNAs) in the midguts of workers at 7 d and 10 d post-inoculation (dpi) with were investigated via small RNA sequencing and bioinformatics. Five hundred and twenty nine (529) known miRNAs and 25 novel miRNAs were identified in this study, and the expression of 16 predicted miRNAs was confirmed by Stem-loop RT-PCR. A total of 14 DEmiRNAs were detected in the midgut at 7 dpi, including eight up-regulated and six down-regulated miRNAs, while 12 DEmiRNAs were observed in the midgut at 10 dpi, including nine up-regulated and three down-regulated ones.

Genome-Wide Identification of Long Non-Coding RNAs and Their Regulatory Networks Involved in Response to Infection.

Long non-coding RNAs (lncRNAs) are a diverse class of transcripts that structurally resemble mRNAs but do not encode proteins, and lncRNAs have been proven to play pivotal roles in a wide range of biological processes in animals and plants. However, knowledge of expression patterns and potential roles of honeybee lncRNA response to infection is completely unknown. Here, we performed whole transcriptome strand-specific RNA sequencing of normal midguts of workers (Am7CK, Am10CK) and -inoculated midguts (Am7T, Am10T), followed by comprehensive analyses using bioinformatic and molecular approaches.

Genome-Wide Identification of Circular RNAs in Fungal Parasite Nosema ceranae.

Circular RNAs (circRNAs) are newly discovered endogenous non-coding RNAs (ncRNAs) that play key roles in microRNA function and transcriptional regulation. Though a large number of circRNAs had been identified in animals and plants, however, little is known regarding circRNAs in Nosema ceranae, a widespread fungal parasite of honeybee. In this study, using deep sequencing technology and bioinformatic analysis, we predicted 204 circRNAs from N.

Systematic investigation of circular RNAs in Ascosphaera apis, a fungal pathogen of honeybee larvae.

Ascosphaera apis is a widespread fungal pathogen of honeybee larvae, which causes heavy losses in apiculture. To date, knowledge about non-coding RNA (ncRNA) including circular RNA (circRNA) in A. apis is lacking.