Potential therapeutic targets for pelvic organ prolapse: insights from key genes related to blood vessel development.

Objective: Pelvic organ prolapse (POP) is a disease in which pelvic floor support structures are dysfunctional due to disruption of the extracellular matrix (ECM). The vascular system is essential for maintaining ECM homeostasis. Therefore, this study explored the potential mechanism of blood vessel development-related genes (BVDRGs) in POP.

Roles and mechanisms of biomechanical-biochemical coupling in pelvic organ prolapse.

Pelvic organ prolapse (POP) is a significant contributor to hysterectomy among middle-aged and elderly women. However, there are challenges in terms of dedicated pharmaceutical solutions and targeted interventions for POP. The primary characteristics of POP include compromised mechanical properties of uterine ligaments and dysfunction within the vaginal support structure, often resulting from delivery-related injuries.

Association of intraoperative hypotension and severe postoperative complications during non-cardiac surgery in adult patients: A systematic review and meta-analysis.

Background: Intraoperative hypotension (IOH) is a common side effect of non-cardiac surgery that might induce poor postoperative outcomes. The relationship between the IOH and severe postoperative complications is still unclear. Thus, we summarized the existing literature to evaluate whether IOH contributes to developing severe postoperative complications during non-cardiac surgery.

[Sorting and identification of human immature Langerhans cells].

Objective To isolate the immature Langerhans cells(LCs) of human epidermis and to identify the phenotype based on their surface marker. Methods After treatment of piecemeal tissues of human foreskin with dispase I digesting overnight, the separated epidermis was further digested by collagenaseIat 37DegreesCelsius in a shaker for 2 hours to produce a suspension of dispersive epithelium, in which, LCs population of CD3CD14CD1aCD45 was harvested in flow cytometry influx with certain antibodies and identified further by immunofluorescence assay of anti-CD207 antibody as while the vitality of these cells were observed with trypan blue staining. Results The population containing 91.