Characterisation of a colourimetric biosensor SapYZUM13@MnO-NH reveals the mechanisms underlying its rapid and sensitive detection of viable Staphylococcus aureus in food.

In this study, a colourimetric biosensor based on bacteriophage SapYZUM13 and an aminated MnO (MnO-NH) nanozyme was constructed and evaluated for its ability to detect Staphylococcus aureus in food. The biosensor had a detection time of 20 min, with a detection limit of 2 × 10 CFU/mL and recovery rate of 92.42-106.

MoO/MIL-125-NH with boosted peroxidase-like activity for electrochemical staphylococcus aureus sensing via specific recognition of bacteriophages.

Herein, novel nanozyme mimics MoO/MIL-125-NH were reported and conjugated with bacteriophages as a new electrochemical probe for high sensitivity and specific electrochemical detection of staphylococcus aureus. The excellent peroxidase-like activity of MoO/MIL-125-NH composites was attributed to the integration of MIL-125-NH with MoO, which can boost the generation of superoxide radicals (O• 2) and thus promote the oxidation of TMB in the presence of HO. In this work, two bacteriophages named SapYZU04 and SapYZU10 were isolated from sewage samples by using staphylococcus aureus YZUsa12 as the host.

Enhanced Antibacterial Efficiency and Anti-Hygroscopicity of Gum Arabic-ε-Polylysine Electrostatic Complexes: Effects of Thermal Induction.

The aim of this investigation was to scrutinize the effects of a thermal treatment on the electrostatic complex formed between gum arabic (GA) and ε-polylysine (ε-PL), with the goal of improving the antibacterial properties and reducing the hygroscopicity of ε-PL. The heated complex with a ratio of 1:4 exhibited an encapsulation efficiency of 93.3%.

Comparative Transcriptomic Analyses Propose the Molecular Regulatory Mechanisms Underlying 1,8-Cineole from Hay and Promote the Asexual Sporulation of in Submerged Fermentation.

is a valuable edible and medicinal mushroom with antitumor, hepatoprotective, and antiviral effects that play a role in intestinal flora regulation. Spore-inoculation submerged fermentation has become the most efficient and well-known artificial culture process for . In this study, a specific low-molecular compound named 1,8-cineole (cineole) from Hay was first reported to have remarkably promoted the asexual sporulation of in submerged fermentation (AcSmF).

Calcium-mediated modulation of Pseudomonas fluorescens biofilm formation.

Biofilm formation is usually affected by many environmental factors, including divalent cations. The purpose of the current work was to analyze how calcium (Ca) affects the biofilm formation of dairy Pseudomonas fluorescens isolates by investigating their growth, swarming motility, biofilm-forming capacity, extracellular polymeric substance production, and biofilm structures. Moreover, the regulation mechanism of Ca involved in its biofilm formation was explored through RNA-sequencing analysis.

WGS analysis of two Staphylococcus aureus bacteriophages from sewage in China provides insights into the genetic feature of highly efficient lytic phages.

The study of bacteriophages is experiencing a resurgence with the increasing development of antimicrobial resistance in Staphylococcus aureus. Nonetheless, the genetic features of highly efficient lytic S. aureus phage remain to be explored.

Molecular Regulatory Mechanism of the Iron-Ion-Promoted Asexual Sporulation of in Submerged Fermentation Revealed by Comparative Transcriptomics.

is a precious edible and medicinal fungus with activities of antitumor, antivirus, and immunoregulation. Fe was found to promote the asexual sporulation of markedly, but the molecular regulatory mechanism of the effect is unclear. In the present study, comparative transcriptomics analysis using RNA sequencing (RNA-seq) and real time quantitative PCR (RT-qPCR) were conducted on mycelia cultured in the presence or absence of Fe to reveal the molecular regulatory mechanisms underlying iron-ion-promoted asexual sporulation.

Review of Bioactivity, Isolation, and Identification of Active Compounds from .

is a precious and popular edible and medicinal mushroom. It has attracted increasing attention due to its various and excellent bioactivities, such as hepatoprotection, hypoglycemic, antioxidant, antitumor, anticancer, anti-inflammatory, immunomodulation, and gut microbiota regulation properties. To elucidate its bioactivities and develop novel functional foods or medicines, numerous studies have focused on the isolation and identification of the bioactive compounds of .

Comparative Transcriptomic Analyses Reveal the Regulatory Mechanism of Nutrient Limitation-Induced Sporulation of in Submerged Fermentation.

is a precious edible and medicinal mushroom with various biological activities, such as hepatoprotection, antitumor, antivirus, immunoregulation, and intestinal flora regulation. However, the wild fruiting bodies of are scarce and expensive. Submerged fermentation based on spore inoculation has become the most efficient and popular artificial culture method for .

Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against spp.

species are important pathogens of marine animals and aquaculture populations and some of them can cause serious infections in humans through consumption of contaminated seafood and aquaculture products. Lytic bacteriophages can potentially alleviate contamination in the aquaculture organisms and in the processing of aquatic products and have gained significant scientific attention in recent years. In the present study, bacteriophages were isolated from sewage of local aquatic products markets and grown using CICC 21613 as host cells.

Prevention and detoxification of patulin in apple and its products: A review.

Patulin-producing fungi pose an unavoidable problem for apple and its product quality, thereby threatening human and/or animal health. Studies on controlling the patulin-producing fungal growth and patulin contamination in apple and its products by physical methods, chemical fungicides, and biological methods have been performed for decades, but patulin contamination has not been addressed. Here, the important of studying regulation mechanism of patulin production in apple at the protein expression and metabolism levels is proposed, which will facilitate the development of controlling patulin production by using physical, chemical, and biological methods.

Enhanced human lysozyme production by Pichia pastoris via periodic glycerol and dissolved oxygen concentrations control.

In human lysozyme (hLYZ) production by Pichia pastoris, the glycerol fed-batch phase was generally implemented under the environment of "oxygen sufficient-glycerol limited" to achieve high cell-density cultivation during the cell growth phase. However, the structural and functional components in P. pastoris cells were irreversible damaged with more and more reactive oxygen species (ROS) accumulation when cells were exposed to the oxygen sufficient environments for long time, leading to a failure of hLYZ expression.

Protective Effect of Spore Powder of ATCC 200183 on CCl-Induced Liver Fibrosis in Mice.

Liver fibrosis is a pathological process with intrahepatic diffused deposition of the excess extracellular matrix, which leads to various chronic liver diseases. Drugs with high efficacy and low toxicity for liver fibrosis are still unavailable. has antioxidant, antivirus, antitumor and anti-inflammation roles, and has been used to treat liver diseases in the population.

Vanillin Promotes the Germination of Arthroconidia through PKA and MAPK Signaling Pathways.

Wild fruiting bodies of medicinal mushroom are only found on the endemic species bull camphor tree, , in Taiwan. Despite the evident importance of the host components in promoting the growth of , insights into the underlying mechanisms are still lacking. Here, we first evaluated effects of the compounds from , and , and their structural analogs on the germination rate of arthroconidia.

[Effect of calcium on sporulation of Taiwanofungus camphoratus in submerged fermentation].

Taiwanofungus camphoratus is a valuable and rare medicinal mushroom with various bioactivities, such as liver protection and anti-cancer. T. camphoratus can produce many arthroconidia at the end of submerged fermentation, but molecular mechanism underlying this submerged conidiation remains unknown.

Comparative Transcriptomic and Proteomic Analyses Reveal a FluG-Mediated Signaling Pathway Relating to Asexual Sporulation of Antrodia camphorata.

Medicinal mushroom Antrodia camphorata sporulate large numbers of arthroconidia in submerged fermentation, which is rarely reported in basidiomycetous fungi. Nevertheless, the molecular mechanisms underlying this asexual sporulation (conidiation) remain unclear. Here, we used comparative transcriptomic and proteomic approaches to elucidate possible signaling pathway relating to the asexual sporulation of A.

Efficient production of bioactive metabolites from Antrodia camphorata ATCC 200183 by asexual reproduction-based repeated batch fermentation.

Large-scale submerged fermentation (SmF) of Antrodia camphorata (A. camphorata) usually encounters challenges including tedious preparation of mycelial inoculum, long fermentation period (10-14 d), and poor repeatability. Here we developed an asexual reproduction-based repeated batch fermentation (RBF) process for bioactive metabolites production by A.

Enhanced conidial thermotolerance and field efficacy of Beauveria bassiana by saccharides.

Insecticide efficacy of Beauveria bassiana conidia was improved by optimizing the concentrations of conidial heat-protective saccharides (glucose, sucrose, maltose, trehalose, α-lactose, and mannitol) using response surface methodology. Two field trials in tea gardens were carried out to control leafhopper (Empoasca vitis) by spraying B. bassiana conidia together with the optimized saccharides (0.

Alpha-terpineol promotes triterpenoid production of Antrodia cinnamomea in submerged culture.

Antrodia cinnamomea is a medicinal mushroom producing potent bioactive triterpenoids. However, triterpenoids of A. cinnamomea in submerged culture are much less than those in fruiting bodies.