Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens.

A large number of viral and bacterial organisms are responsible for community-acquired pneumonia (CAP) which contributes to substantial burden on health management. A new resequencing microarray (RPM-IVDC1) associated with targeted multiplex PCR was recently developed and validated for multiple respiratory viruses detection and discrimination. In this study, we evaluated the capability of RPM-IVDC1 for simultaneous identification of multiple viral and bacterial organisms.

[Study on the serotype, virulence genes and pulsed field gel electrophoresis molecular typing of Vibrio parahaemolyticus isolated from clinical patients].

Objective: To investigate the serotype virulence genes and molecular typing characteristics of Vibrio Parahaemolyticus isolated from diarrhea samples among 2007 -2012.

Methods: The serotype was detected by using serological agglutination test. Thermo-stable direct hemolysin (tdh) and tdh-related hemolysin (trh) were detected by using real time PCR methods.