Publications by authors named "Huanling Li"

Postharvest pericarp browning, caused primarily by the enzymatic oxidation of phenols, reduces the shelf life and market value of litchi fruit and is considered a major limitation for the development of the litchi industry. Previous studies have shown that polyphenol oxidase (PPO) is a key enzyme and that flavonoids are important substrates for enzymatic browning; however, direct evidence is still lacking. This study investigated the differences in the browning process among the wild type (WT) and four PPO gene-edited litchi calli to verify the function of PPO in the browning of litchi tissues.

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Anthocyanidin reductase () is a key enzyme regulating anthocyanin synthesis and accumulation in plants. Here, lychee genes were globally identified, their sequence and phylogenetic characteristics were analyzed, and their spatiotemporal expression patterns were characterized. A total of 51 family members were identified in the lychee genome.

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D-arginine (D-Arg) can promote embryogenic callus (EC) proliferation and increase the rate of somatic embryo induction of litchi ( Sonn.), yet the mechanism underlying the processes is incompletely understood. To investigate the mechanism, physiological responses of polyamines (PAs) [putrescine (Put), spermidine (Spd), and spermine (Spm)] were investigated for D-Arg-treated litchi EC and enzyme activity related to polyamine metabolism, plant endogenous hormones, and polyamine- and embryogenic-related genes were explored.

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Biofouling and bacterial infections are significant challenges in biomedical devices. In this study, a biocompatible dual-functional coating with antimicrobial and antifouling properties is developed by co-depositing the zwitterionic copolymer and silver nanoparticles via a dopamine-assisted strategy. Inspired by mussel adhesion, the coating exhibits substrate-independent adhesion as a result of the formation of irreversible covalent bonds.

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Article Synopsis
  • * The study focused on identifying the pathogens causing anthracnose disease in litchi trees in Hainan, China, utilizing both genetic and morphological analysis methods.
  • * Eight distinct fungal species linked to anthracnose were identified, including a new species, with all species showing the ability to infect litchi leaves, though their infectivity varied.
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Background: Efficient deposition of high-speed droplets on superhydrophobic leaf surfaces remains an important challenge. For anisotropic wired superhydrophobic leaf surfaces, the splashing phenomenon is especially serious because it leads to the low effective utilization of pesticides by biological targets. The lost pesticides cause serious ecological environment pollution, therefore there is an urgent need to develop a green and sustainable cost-effective strategy to achieve efficient deposition of high-speed droplets on anisotropic superhydrophobic leaf surfaces at low dosage.

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Although many studies have focused on oncology and therapeutics in cancer, cancer remains one of the leading causes of death worldwide. Due to the unclear molecular mechanism and complex in vivo microenvironment of tumors, it is challenging to reveal the nature of cancer and develop effective therapeutics. Therefore, the development of new methods to explore the role of heterogeneous TME in individual patients' cancer drug response is urgently needed and critical for the effective therapeutic management of cancer.

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Background: The aim of this study was to investigate the value of electrocardiograms (ECGs) and serological examinations in the differential diagnosis of acute pulmonary embolism (APE) and acute non-ST elevation myocardial infarction (NSTEMI) in order to reduce the rate of clinical misdiagnosis.

Methods: The clinical data of 37 patients with APE and 103 patients with NSTEMI admitted to our hospital were retrospectively analyzed. The differences in the clinical manifestations, ECGs, myocardial zymograms, D-dimers, and troponin (cTn) of the two groups were compared.

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Continuous morphological control of anisotropic particles is always an important challenge in the field of materials. In this study, a new strategy for continuous fabrication of polymer particles with various morphologies induced by electricity is reported using complex emulsions as template. A synthetic electro-responsive surfactant containing ferrocene group is used to prepare complex emulsions, which contain a polymerizable monomer as inner phase.

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Litchi (Litchi chinensis Sonn.) is an indigenous tropical and subtropical fruit in Southern China with an attractive appearance, delicious taste, and good nutritional value (Jiang et al. 2003).

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In February 2020, the common symptoms of water-soaked spots on Cucurbita pepo L. cotyledon were observed in Guangrao county in Shandong province, China. Field investigation showed that 40% of the Cucurbita pepo cotyledons in an area of approximately 0.

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Near-infrared (NIR) fluorescent probes are among the most attractive chemical tools for biomedical imaging. However, their in vivo applications are hindered by albumin binding, generating unspecific fluorescence that masks the specific signal from the analyte. Here, combining experimental and docking methods, we elucidate that the reason for this problem is an acceptor (A) group-mediated capture of the dyes into hydrophobic pockets of albumin.

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A lignin-biochar with high acidic oxygen-containing groups was produced via a facile simultaneous oxidization and carbonization of pulping lignin by using sulfuric acid for the first time. The lignin-biochars were investigated by C NMR, FTIR and XPS, and results demonstrated that treatment by sulfuric acid could oxidize part of aromatic ring side chain to introduce high acidic oxygen-containing group, especially carboxyl group. Their total acidic oxygen-containing groups of lignin-biochar including COOH and Ar-OH reached 8.

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The rate-limiting step in cutaneous wound healing, namely, the transition from inflammation to cell proliferation, depends on the high plasticity of macrophages to prevent inflammation in the wound tissues in a timely manner. Thus, strategies that reprogram inflammatory macrophages may improve the healing of poor wounds, particularly in the aged skin of individuals with diabetes or other chronic diseases. As shown in our previous study, KGM-modified SiO nanoparticles (KSiNPs) effectively activate macrophages to differentiate into the M2-type phenotype by inducing mannose receptor (MR) clustering on the cell surface.

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Litchi downy blight, a destructive litchi disease caused by Peronophythora litchii, is controlled by intensive fungicide applying. Sources of resistance are used in conventional breeding approaches, but the mechanism is not well understood. Follow-up six years investigation, 'Guiwei' and 'Heiye' displayed stable susceptible and resistant against to P.

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MicroRNAs (miRNAs) are important regulators in tumorigenesis and progression of multiple human cancers, including ovarian cancer (OC). As a member of miRNAs family, miR-374b-5p has been reported to be a tumor suppressive gene in human cancers. In this study, the lower expression of miR-374b-5p was identified in OC tissues and cell liens using quantitative real time PCR (qRT-PCR).

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Polyphenol oxidase (PPO) plays a key role in the postharvest pericarp browning of litchi fruit, but its underlying mechanism remains unclear. In this study, we cloned the litchi PPO gene (LcPPO, JF926153), and described its expression patterns. The LcPPO cDNA sequence was 2120 bps in length with an open reading frame (ORF) of 1800 bps.

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