Publications by authors named "Huanhuan Zong"
Single nucleotide polymorphisms (SNPs) in homologous regions play a critical role in the field of genetics. However, genotyping these SNPs is challenging due to the presence of repetitive sequences within genome, which demand specific method. We introduce a new, mid-throughput method that simplifies SNP genotyping in homologous DNA sequences by utilizing a combination of multiplex kb level PCR (PCR size 2.
View Article and Find Full Text PDFRapid Real-Time PCR Based on Core-Shell Tecto-Dendrimer-Entrapped Au Nanoparticles.
ACS Biomater Sci Eng
October 2024
Article Synopsis
- Researchers developed a new nanomaterial called Au CSTDs, which are core-shell tecto-dendrimers coated with gold nanoparticles, aimed at improving rapid real-time PCR processes.
- The study found that using Au CSTDs reduced the PCR time from 72 minutes to just 28 minutes and achieved a detection limit of 10 copies while minimizing nonspecific amplification.
- Theoretical and experimental analysis indicated that Au CSTDs enhance PCR efficiency by improving electrostatic adsorption and thermal conductivity, making them a promising advancement in molecular detection techniques.
Hypoxia can lead to stabilization of the tumor suppressor gene p53 and cell death. However, p53 mutations could promote cell survival in a hypoxic environment. In this study, we found that p53N236S (p53N239S in humans, hereinafter referred to as p53S) mutant mouse embryonic fibroblasts (MEFs) resistant to deferoxamine (DFO) mimic a hypoxic environment.
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