Modification of nanoscale polymeric adsorbent for the preparative separation and purification of tacrolimus from fermentation broth of Streptomyces tsukubaensis.

Tacrolimus is an important immunosuppressant produced by microbial fermentation. In this study, a modified nanoscale polymeric adsorbent, Ag-exchanged resin, was prepared and studied for the preparative separation and purification of tacrolimus from fermentation broth of Streptomyces tsukubaensis. The performance and absorption characteristics of the modified nanoscale polymeric adsorbent namely Ag-NPS was evaluated.

Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.

Objective: This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.

Results: A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.

Two novel amphomycin analogues from Streptomyces canus strain FIM-0916.

Three lipopeptides, the known compound amphomycin, together with two novel compounds named aspartocin D (1) and aspartocin E (2) were obtained from the fermentation broth extraction of Streptomyces canus strain FIM0916 by using various column chromatography techniques. Their structures were elucidated by using spectroscopic methods, mainly by an extensive NMR analysis. It was demonstrated that compounds 1 and 2 are novel analogues of amphomycin, whose structures are similar to aspartocins.

Two novel ansamitocin analogs from Actinosynnema pretiosum.

By using various column chromatography for purification, two new compounds of ansamitocin (1 and 2) were isolated from the extracts of fermentation broth of Actinosynnema pretiosum FIM06-0063. Their structures were established as maytansinol-9-methyl ether-3-propionate (1) and maytansinol-9-methyl ether-3-3'-methy-butyrate (2), respectively, by an extensive NMR analysis.

Effects of zinc and manganese on advanced glycation end products (AGEs) formation and AGEs-mediated endothelial cell dysfunction.

Aims: The present study investigated the effects of ZnCl₂ and MnCl₂ supplementations on advanced glycation end products (AGEs) formation and AGEs-mediated endothelial cell dysfunction.

Main Methods: Fluorescence detection was used to monitor the Maillard reaction. Inductively coupled plasma optical emission spectroscopy was used to test cellular zinc and manganese levels.

[Experimental study of saponins from Tribulus terrestris on renal carcinoma cell line].

Objective: To investigate the effect of saponins from Tribulus terrestris (STT) on the renal carcinoma cell (786-0) in vitro, and inhibitory mechanisms.

Method: Effects of SIT on the cytotoxicity, morphological changes of apoptosis, cell cycle and expression of Bcl-2 protein in the 786-0 were tested respectively by MTT method, Wright and acridine orange stain assay, as well as flow cytometry (FCM).

Result: After the 786-0 was treated by STY, it was shown that: 1) A significant cytotoxic effect was observed by MTT assay; 2) Apoptosis-induced was viewed by Wright and acridine orange stain assay; 3) The distribution of 786-0 on S phase was increased; 4.

[Investigation on inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris on hepatoma cell line BEL-7402].

Objective: To investigate the inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris (STT) on liver cancer cell line BEL-7402.

Method: MTT, SRB, Wright staining, acridine orange staining, flow cytometry, and Immunofluorescence microscopy were used to evaluate the effects of STT on BEL-7402 cell line.

Result: SMT had potent inhibitory effect on BEL-7402 cell line in a concentration-dependent manner.

[Study on effect of flavonoids from oil-removed seeds of Hippophae rhamnoides on inducing apoptosis of human hepatoma cell].

Objective: To investigate the inhibitory and apoptosis-inducing effects of flavonoids from oil-removed seeds of Hippophae rhamnoides (FSH) on liver cancer cell line BEL-7402.

Methods: SRB, Wright staining, electron microscope and flow cytometry are used to study the effects of STT on BEL-7402 cell line.

Results: FSH has potent inhibitive effect on BEL-7402 cell line in a concentration-dependent manner.