Facile Synthesis of Thermoresponsive Alternating Copolymers with Tunable Phase-Transition Temperatures.

A series of novel amphiphilic alternating CPEG copolymers were synthesized through an amine-epoxy click reaction comprising aliphatic amine and polyethylene glycol diglycidyl ether (PEGDE). These polymers were characterized in detail via nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) to confirm the successful synthesis. Due to their amphiphilic structure, these polymers display thermoresponsiveness, with tunable cloud points (Tcps) that are adjustable from 20.

Identification and Characterization of EIN3/EIL Transcription Factor Family Members in Lamb.

Transcription factors refer to types of proteins that perform significant functions in the process of gene expression regulation. The ethylene insensitive 3/ethylene insensitive 3-like () family, functioning as significant transcription factors regulating ethylene, plays a critical role in the growth and development of plants and participates in the plant's response to diverse environmental stresses. is an excellent native tree with high economic and ecological value.

Effect of incision location and type of fistula on postoperative urinary retention after radical surgery for anal fistula: a retrospective analysis.

Background: Postoperative urinary retention (POUR) is a common complication characterized by fullness of the bladder without the ability to urinate. Its etiology in proctology surgery is multifactorial. This study aimed to identify the risk factors for POUR after radical surgery for anal fistula.

Analysis on metabolic functions of rhizosphere microbial communities of provenances with different carbon storage by Biolog Eco microplates.

Introduction: Rhizosphere microorganisms are influenced by vegetation. Meanwhile, they respond to vegetation through their own changes, developing an interactive feedback system between microorganisms and vegetation. However, it is still unclear whether the functional diversity of rhizosphere soil microorganisms varies with different carbon storage levels and what factors affect the functional diversity of rhizosphere soil microorganisms.

Whole-transcriptome analysis reveals mechanisms underlying antibacterial activity and biofilm inhibition by a malic acid combination (MAC) in .

Background: is a highly prevalent bacterial species known for its ability to cause various infections and its remarkable adaptability and biofilm-forming capabilities. In earlier work, we conducted research involving the screening of 33 metabolites obtained from a commercial source against two prevalent bacterial strains, and . Through screening assays, we discovered a novel malic acid combination (MAC) consisting of malic acid, citric acid, glycine, and hippuric acid, which displayed significant inhibitory effects.

Microbial communities and functions changed in rhizosphere soil of provenances with different carbon storage.

Introduction: The average carbon storage of is much higher than the average carbon storage of Chinese forests, an important carbon sink tree species in subtropical regions of China. However, there are few studies on the differences in rhizosphere microorganisms of with different carbon storages.

Methods: To clarify the relationships between plant carbon storage level, environmental parameters and microbial community structure, we identified three carbon storage levels from different provenances and collected rhizosphere soil samples.

Transcriptome-Wide Identification of TCP Transcription Factor Family Members in and Their Expression in Regulation of Development and in Response to Stress.

is an important coniferous tree species for barren mountain afforestation with enormous ecological and economic significance. It has strong adaptability to the environment. TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) play crucial roles in plant stress response, hormone signal transduction, and development processes.

Investigating the characteristics of fluorescence features on sweet peppers using UV light excitation.

Sweet peppers are popular worldwide due to their nutrition and taste. Conventional vegetable tracing methods have been trialed, but the application of such labels or tags can be laborious and expensive, making their commercial application impractical. What is needed is a label-free method that can identify features unique to each individual fruit.

Two-finger digital rectal examination for the diagnosis of anal fistula: protocol for a randomized controlled trial.

Background: Anal fistula is an anorectal infectious disease caused by a perianal abscess or perianal disease. Accurate anorectal examinations are of great significance. The two-finger digital rectal examination (TF-DRE) has been used in clinical practice, with a lack of comprehensive research on the value of the TF-DRE in the diagnosis of anal fistula.

A Versatile Design-Enabled Analysis of Circulating Extracellular Vesicles in Disease Diagnosis.

Circulating extracellular vesicles (EVs) are considered as potential biomarkers for treatment and diagnosis of many diseases. Most of the existing methods for the EV analysis only have a single function and thus reveal limited information carried by EVs. Herein, a phosphatidylserine-targeting peptide-facilitated design that enables the versatile analysis of circulating EVs for varying requirement is proposed.

Macroscopic and microscopic characterization of fluorescence properties of multiple sweet pepper cultivars (Capsicum annuum L.) using excitation-emission matrix and UV induced fluorescence imaging.

Sweet peppers are a popular vegetable with various surface colors, such as green, purple, red, or yellow. To characterize the unique fluorescence properties associated with a broad range of sweet peppers of various colors (14 varieties), a fluorescence spectrofluorometer and imaging were used. The results showed that all cultivars in the experiment had blue fluorescence emissions when excited with light in the UV-A region, while chlorophyll fluorescence could be observed in green peppers.

Tactile Perception Object Recognition Based on an Improved Support Vector Machine.

Tactile perception is an irreplaceable source of information for humans to explore the surrounding environment and has advantages over sight and hearing in processing the material properties and detailed shapes of objects. However, with the increasing uncertainty and complexity of tactile perception features, it is often difficult to collect highly available pure tactile datasets for research in the field of tactile perception. Here, we have proposed a method for object recognition on a purely tactile dataset and provide the original tactile dataset.

Transcriptome-Wide Identification of CCCH-Type Zinc Finger Proteins Family in and RR-TZF Proteins in Stress Response.

CCCH-type zinc finger proteins play an important role in multiple biotic and abiotic stresses. More and more reports about CCCH functions in plant development and stress responses have appeared over the past few years, focusing especially on tandem CCCH zinc finger proteins (TZFs). However, this has not been reported in Pinaceae.

Clustering by measuring local direction centrality for data with heterogeneous density and weak connectivity.

Clustering is a powerful machine learning method for discovering similar patterns according to the proximity of elements in feature space. It is widely used in computer science, bioscience, geoscience, and economics. Although the state-of-the-art partition-based and connectivity-based clustering methods have been developed, weak connectivity and heterogeneous density in data impede their effectiveness.

Japanese dace (Tribolodon hakonensis) fish freshness estimation using front-face fluorescence spectroscopy coupled with chemometric analysis.

Although fish and its related products are good sources of protein and unsaturated fatty acids, like omega-3 in the human diet, their shelf-life is limited by biochemical and microbial changes. In this study, a front-face fluorescence spectroscopy technique was used to acquire Excitation-emission matrices (EEM) to monitor Japanese dace (Tribolodon hakonensis) fish freshness degradation during storage. EEM of Japanese dace fish parts (intact eyeball and surface-containing scales), excitation from 220 to 585 nm and emissions from 250 to 600 nm, were measured at different times during storage.

Close interactions between lncRNAs, lipid metabolism and ferroptosis in cancer.

Abnormal lipid metabolism including synthesis, uptake, modification, degradation and transport has been considered a hallmark of malignant tumors and contributes to the supply of substances and energy for rapid cell growth. Meanwhile, abnormal lipid metabolism is also associated with lipid peroxidation, which plays an important role in a newly discovered type of regulated cell death termed ferroptosis. Long noncoding RNAs (lncRNAs) have been proven to be associated with the occurrence and progression of cancer.

Intercropping improves heavy metal phytoremediation efficiency through changing properties of rhizosphere soil in bamboo plantation.

Moso bamboo is considered a potential species for heavy metal (HM) phytoremediation; however, the effect of intercropping on rhizosphere and phytoextraction remains to be elucidated. We comparatively investigated rhizobacteria, soil properties, and phytoextraction efficiency of monoculture and intercropping of Moso bamboo and Sedum plumbizincicola in Cu/Zn/Cd-contaminated soil. Compared with monocultures, intercropping increased the bacterial α-diversity indices (Shannon, Chao1) and the number of biomarkers.

Long-term follow-up study of loose combined cutting seton surgery for patients with high anal fistula.

Background: The treatment of high anal fistula (HAF) is still difficult for clinical surgeons. Our previous study demonstrated the short-term benefit of loose combined cutting seton (LCCS) for patients with HAF. This study aimed to evaluate the long-term effectiveness of LCCS for treating HAF patients.

UV excited fluorescence image-based non-destructive method for early detection of strawberry (Fragaria × ananassa) spoilage.

The soon spoiled strawberries need to be classified from healthy fruits in an early stage. In this research, a machine vision system is proposed for inspecting the quality of strawberries using ultraviolet (UV) light based on the excitation-emission matrix (EEM) results. Among the 100 fruits which were harvested and stored under 10 °C condition for 7 days, 7 fruits were confirmed to be spoiled by using a firmness meter.

Soil carbon and associated bacterial community shifts driven by fine root traits along a chronosequence of Moso bamboo (Phyllostachys edulis) plantations in subtropical China.

Moso bamboo (Phyllostachys edulis) is widely considered to be effective in capturing and sequestering atmospheric C, but the long-term effects of extensive management strategies on soil organic carbon (SOC), bacterial communities, fine root (FR, ø ≤ 2 mm) traits, and their inherent connection remain unclear. In this study, we simultaneously measured the SOC content of the bulk and rhizosphere soil fractions, the aggregate stability, the chemical composition of SOC (solid-state C nuclear magnetic resonance [NMR]), the bacterial community structure in the rhizosphere, and the FR morphological traits including biomass, specific root length (SRL), and root length density (RLD) along a chronosequence (stand age of 19, 37, and 64 years) of extensively managed Moso bamboo plantations and in an adjacent secondary forest as a control. The organic C content in both the rhizosphere and bulk soil increased rapidly with plantation age in the 0-20- and 20-40-cm soil layers, accompanied by an increase in the aggregate stability.

Loose combined cutting seton for patients with high intersphincteric fistula: a retrospective study.

Background: Achieving a complete cure while maintaining continence constitutes a considerable challenge in the treatment of patients with high anal fistula. This study aimed to evaluate the effectiveness of loose combined cutting seton (LCCS) for treating patients with high intersphincteric fistula.

Methods: Consecutive patients with high intersphincteric fistula who underwent LCCS were retrospectively enrolled.

Potato (Solanum tuberosum L.) tuber-root modeling method based on physical properties.

The development of tuber-root models based on the physical properties of the root system of a plant is a prominent but complicated task. In this paper, a method for the construction of a 3D model of a potato tuber-root system is proposed, based on determining the characterization parameters of the potato tuber-root model. Three early maturing potato varieties, widely planted in Northeast China, were selected as the research objects.

A Noise Tolerant Spread Spectrum Sound-Based Local Positioning System for Operating a Quadcopter in a Greenhouse.

Quadcopters are beginning to play an important role in precision agriculture. In order to localize and operate the quadcopter automatically in complex agricultural settings, such as a greenhouse, a robust positioning system is needed. In previous research, we developed a spread spectrum sound-based local positioning system (SSSLPS) with a 20 mm accuracy within a 30 × 30 m greenhouse area.