Understanding perspectives and research trends in Down syndrome neuropathogenesis: A bibliometric analysis.

Down syndrome (DS), characterised by compromised brain development and intellectual challenges, often manifests Alzheimer's disease (AD) -like symptoms. Utilising the Web of Science Core Collection (WOSCC) database from January 1, 2000, to July 31, 2023, we conducted a comprehensive bibliometric analysis using VOSviewer, CiteSpace, and the R package "bibliometrix." Analyses included co-authorship, co-citation, co-occurrence, cooperative network, reference, and keyword burst citation.

Chromosomal and cellular therapeutic approaches for Down syndrome: A research update.

In individuals with Down syndrome (DS), an additional HSA21 chromosome copy leads to the overexpression of a myriad of HSA21 genes, disrupting the transcription of the entire genome. This dysregulation in transcription and post-transcriptional modifications contributes to abnormal phenotypes across nearly all tissues and organs in DS individuals. The array of severe clinical symptoms associated with trisomy 21 poses a considerable challenge in the quest for a cure for DS.

The "Pull Effect" of a Hanging Zn on Improving the Four-Electron Oxygen Reduction Selectivity with Co Porphyrin.

Due to the challenge of cleaving O-O bonds at single Co sites, mononuclear Co complexes typically show poor selectivity for the four-electron (4e) oxygen reduction reaction (ORR). Herein, we report on selective 4e ORR catalyzed by a Co porphyrin with a hanged Zn ion. Inspired by Cu/Zn-superoxide dismutase, we designed and synthesized 1-CoZn with a hanging Zn at the second sphere of a Co porphyrin.

The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana.

The Ti-TAN TurboID plasmid toolbox enables proximity labeling applications in transient assays in Nicotiana benthamiana in a fast and cost-efficient manner, making TurboID-based proximity labeling broadly accessible to plant scientists.

Anchoring Fe Species on the Highly Curved Surface of S and N Co-Doped Carbonaceous Nanosprings for Oxygen Electrocatalysis and a Flexible Zinc-Air Battery.

Oxygen reduction reaction (ORR) is of critical significance in the advancement of fuel cells and zinc-air batteries. The iron-nitrogen (Fe-N ) sites exhibited exceptional reactivity towards ORR. However, the task of designing and controlling the local structure of Fe species for high ORR activity and stability remains a challenge.

Split-Luciferase Complementation Imaging Assay in Virus-Plant Interactions.

Protein-protein interactions constitute the interface between a virus and the cell it infects and are crucial determinants of the outcome of the viral infection. Multiple techniques have been developed to study how viral and host proteins interact in plants; among them, the split-luciferase complementation imaging assay stands out due to its capacity to detect protein-protein interactions in vivo, in the context of the infection, if desired, in an easy, fast, quantitative, and inexpensive manner. In this chapter, we use the interaction between the V2 protein from the geminivirus tomato yellow leaf curl virus (TYLCV) and Nicotiana benthamiana Argonaute 4 (AGO4) as an example to present how to perform this simple yet powerful assay using transient Agrobacterium tumefaciens-mediated transformation of N.

Structurally-Distorted RuIr-Based Nanoframes for Long-Duration Oxygen Evolution Catalysis.

Oxygen evolution reaction (OER) plays a key role in proton exchange membrane water electrolysis (PEMWE), yet the electrocatalysts still suffer from the disadvantages of low activity and poor stability in acidic conditions. Here, a new class of CdRu IrO nanoframes with distorted structure for acidic OER is successfully fabricated. Impressively, CdRu IrO displays an ultralow overpotential of 189 mV and an ultralong stability of 1500 h at 10 mA cm⁻ toward OER in 0.

REST Targets JAK-STAT and HIF-1 Signaling Pathways in Human Down Syndrome Brain and Neural Cells.

Down syndrome (DS) is the most frequently diagnosed chromosomal disorder of chromosome 21 (HSA21) aneuploidy, characterized by intellectual disability and reduced lifespan. The transcription repressor, Repressor Element-1 Silencing Transcription factor (REST), which acts as an epigenetic regulator, is a crucial regulator of neuronal and glial gene expression. In this study, we identified and investigated the role of REST-target genes in human brain tissues, cerebral organoids, and neural cells in Down syndrome.

CoO Supported on Graphene-like Carbon by One-Step Calcination of Cobalt Phthalocyanine for Efficient Oxygen Reduction Reaction under Alkaline Medium.

Exploiting cost-effective and durable non-platinum electrocatalysts for oxygen reduction reaction (ORR) is of great significance for the development of abundant renewable energy conversion and storage technologies. Herein, a series of CoO supported on graphene-like carbon (CoO/C) samples were firstly effectively synthesized by one-step calcination of cobalt phthalocyanine and their electrocatalytic performances were measured for ORR under an alkaline medium. By systematically adjusting the calcination temperature of cobalt phthalocyanine, we found that the material pyrolyzed at 750 °C (CoO/C-750) shows the best ORR electrocatalytic performance (half-wave potentials of 0.

Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome.

Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. Here, we explore the hypothesis that combinatorial interactions may expand the functional landscape of the viral proteome.

Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells.

The availability of a fast and controlled mitotic model system that could simplify the generation of genetic material and reduce the experimental time from months to days would largely benefit research in plant cell division. In this protocol, we propose the use of pavement cells of Nicotiana benthamiana leaves to study cell division, which is artificially induced by Agrobacterium-mediated transient overexpression of the transcription factor E2Fb. The cell division-inducing overexpression of E2Fb can be combined with the expression of fluorescent protein-tagged proteins of interest or with dyes, which could be visualized throughout the cell cycle under the microscope.

Geminiviruses encode additional small proteins with specific subcellular localizations and virulence function.

Geminiviruses are plant viruses with limited coding capacity. Geminivirus-encoded proteins are traditionally identified by applying a 10-kDa arbitrary threshold; however, it is increasingly clear that small proteins play relevant roles in biological systems, which calls for the reconsideration of this criterion. Here, we show that geminiviral genomes contain additional ORFs.

Plant DNA polymerases α and δ mediate replication of geminiviruses.

Geminiviruses are causal agents of devastating diseases in crops. Geminiviruses have circular single-stranded (ss) DNA genomes that are replicated in the nucleus of the infected plant cell through double-stranded (ds) DNA intermediates by the plant DNA replication machinery. Which host DNA polymerase mediates geminiviral multiplication, however, has so far remained elusive.

A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens.

Chloroplasts are crucial players in the activation of defensive hormonal responses during plant-pathogen interactions. Here, we show that a plant virus-encoded protein re-localizes from the plasma membrane to chloroplasts upon activation of plant defense, interfering with the chloroplast-dependent anti-viral salicylic acid (SA) biosynthesis. Strikingly, we have found that plant pathogens from different kingdoms seem to have convergently evolved to target chloroplasts and impair SA-dependent defenses following an association with membranes, which relies on the co-existence of two subcellular targeting signals, an N-myristoylation site and a chloroplast transit peptide.

A facile strategy towards heterogeneous preparation of thermoplastic cellulose grafted polyurethane from amorphous regenerated cellulose paste.

Cellulose is an abundant feedstock with renewability and biodegradability. However, it is still challenging to manufacture natural cellulose products by environmentally friendly thermoplastic processing methods. Herein, we proposed a green approach for the heterogeneous preparation of thermoplastic cellulose grafted polyurethane (RCP-g-PU) from amorphous regenerated cellulose paste (RCP) via hydroxyl/isocyanate chemistry.

Transient overexpression of E2Fb triggers cell divisions in pavement cells of Nicotiana benthamiana leaves.

Agrobacterium-mediated transient overexpression of E2Fb triggers new cell divisions in pavement cells of Nicotiana benthamiana leaves. Transient transformation in Nicotiana benthamiana enables the study of multiple biological processes in a simple and fast manner. Here, we describe that, upon A.

Three-Dimensional Graphene-Supported NiFe/CoS Composites: Rational Design and Active for Oxygen Reversible Electrocatalysis.

The development of low-cost and efficient electrocatalysts with a bicomponent active surface for reversible oxygen electrode reactions is highly desirable and challenging. Herein, we develop an effective calcination-hydrothermal approach to fabricate graphene aerogel-anchored NiFe-CoS bifunctional electrocatalyst (NiFe-CoS/rGO). The mutually beneficial NiFe-CoS bifunctional active components efficiently balance the performance of oxygen reduction and oxygen evolution reactions (ORR/OER), in which CoS promotes the ORR and NiFe facilitates the OER.

Dynamic Virus-Dependent Subnuclear Localization of the Capsid Protein from a Geminivirus.

Viruses are intracellular parasites with a nucleic acid genome and a proteinaceous capsid. Viral capsids are formed of at least one virus-encoded capsid protein (CP), which is often multifunctional, playing additional non-structural roles during the infection cycle. In animal viruses, there are examples of differential localization of CPs associated to the progression of the infection and/or enabled by other viral proteins; these changes in the distribution of CPs may ultimately regulate the involvement of these proteins in different viral functions.

A Microribbon Hybrid Structure of CoOx-MoC Encapsulated in N-Doped Carbon Nanowire Derived from MOF as Efficient Oxygen Evolution Electrocatalysts.

Developing highly efficient electrocatalysts for oxygen evolution is vital for renewable and sustainable energy production and storage. Herein, nitrogen-doped carbon encapsulated CoOx-MoC heterostructures are reported for the first time as high performance oxygen evolution electrocatalysts. The composition can be tuned by the addition of a Mo source to form a nanowire-assembled hierarchically porous microstructure, which can enlarge the specific surface area, thus exposing more active sites, facilitating mass transport and charge transfer.

Single-Crystalline InGaAs Nanowires for Room-Temperature High-Performance Near-Infrared Photodetectors.

InGaAs is an important bandgap-variable ternary semiconductor which has wide applications in electronics and optoelectronics. In this work, single-crystal InGaAs nanowires were synthesized by a chemical vapor deposition method. Photoluminescence measurements indicate the InGaAs nanowires have strong light emission in near-infrared region.