Nanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes.

Antibiotic resistance genes (ARGs) are markers of drug-resistant pathogens, monitoring them contributes to prevent resistance to drugs. The detection methods for ARGs including PCR and isothermal amplification are sensitive and selective. However, it may take several hours or cannot be used on spot.

The Effect of Cryopreservation on T-Cell Subsets by Flow Cytometry Automated Algorithmic Analysis and Conventional Analysis.

Background: Low-temperature cryopreservation is a common method for scientific research and clinical sample preservation when utilizing flow cytometry. In flow cytometry data analysis, traditional manual "gating" is susceptible to past experience and faces the challenge of manual subjective bias, time-consuming, and multidimensional data analysis. With the development of algorithms, the advantages of dimensionality reduction and clustering in result analysis are gradually becoming more prominent.

Depression among Tibetan residents in the Southeastern region of Qinghai-Tibet plateau: a cross-sectional study.

Depression has emerged as a significant public health concern, with its prevalence fluctuating based on varying environmental and demographic factors. This study categorized participants based on altitude. A convenient sampling approach was used, and the hamilton depression rating scale-24 was used to assess depressed symptoms while gathering demographic information.

Floatable artificial leaf to couple oxygen-tolerant CO conversion with water purification.

To enable open environment application of artificial photosynthesis, the direct utilization of environmental CO via an oxygen-tolerant reductive procedure is necessary. Herein, we introduce an in situ growth strategy for fabricating two-dimensional heterojunctions between indium porphyrin metal-organic framework (In-MOF) and single-layer graphene oxide (GO). Upon illumination, the In-MOF/GO heterostructure facilitates a tandem CO capture and photocatalytic reduction on its hydroxylated In-node, prioritizing the reduction of dilute CO even in the presence of air-level O.

Trivalent recombinant protein vaccine induces cross-neutralization against XBB lineage and JN.1 subvariants: preclinical and phase 1 clinical trials.

The immune escape capacities of XBB variants necessitate the authorization of vaccines with these antigens. In this study, we produce three recombinant trimeric proteins from the RBD sequences of Delta, BA.5, and XBB.

Alkalinity enhanced hydrolysis of primary sludge for carbon source recovery and its impact on denitrification in wastewater treatment.

Primary sludge can serve as an internal carbon source for denitrification in wastewater treatment plants (WWTPs). This study explores the use of alkaline treatment to produce a fermentation broth from primary sludge, which predominantly contains short-chain volatile fatty acids (VFAs), with acetic acid and propionic acid making up over 65% of the total VFAs. The performance of this fermentation broth as a sole carbon source for denitrification was compared with that of sodium acetate, acetic acid, methanol, and ethanol in both biofilm and activated sludge systems.

Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection.

A novel carbon-based light-addressable potentiometric aptasensor (C-LAPS) was constructed for detection low-density lipoprotein (LDL) in serum. Carboxylated TiC MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantum dots (OPD@NGQDs) as the photoelectric conversion element. The photosensitive layers composed of OPD@NGQDs/C-MXene@rGO exhibit superior photoelectric conversion efficiency and excellent biocompatibility, which contribute to an improved response signal.

Applications of low-temperature plasma technology in microalgae cultivation and mutant breeding: A comprehensive review.

Low-temperature plasma (LTP) has gained significant attention recently due to its unique properties and potentially wide applications in agriculture, medicine, and food industry. Microalgae have become important to human life since they provide raw materials and bioactive products to industries. This review especially examines how LTP technology can be utilized to enhance microalgae growth and production of various metabolites and bioactive compounds such as astaxanthin, biofuel, lipid, proteins, and polysaccharides through mutagenesis and/or stimulation.

Thyroid metastases from cervical carcinoma: unusual implications for clinicians-case report.

Background: Thyroid metastases from uterine cervical carcinoma are exceedingly rare. Imaging examination and pathological detection exert a pivotal function in distinguishing between benign and malignant thyroid tumors, as well as differentiating metastases from primary thyroid cancer.

Case Presentation: Here, we present two cases of uterine cervical cancer metastasizing to the thyroid gland, confirmed by postoperative histopathology at our hospital.

Effect of degrees of milling on the volatile compounds of cooked fragrant Simiao rice: differential volatiles obtained by GC-MS-based untargeted metabolomics.

Background: Alterations in the degrees of milling (DOM) could significantly influence the odor of rice. A gas chromatography-mass spectrometry (GC-MS)-based untargeted metabolomics method has been effectively employed to identify the differential volatiles among rice from various origins or varieties, although it has not been utilized to identify the differential volatiles among cooked rice with different DOM.

Results: Fifty volatile compounds were detected in cooked brown rice (CBR), cooked medium-milled rice (CMMR) and cooked well-milled rice (CWMR) of the four fragrant Simiao rice by GC-MS.

Effects of Preformed Composition and Pore Size on Microstructure and Properties of SiC/SiC Composites via Reactive Melt Infiltration.

This study investigated the influence of preformed composition and pore size on the microstructure and properties of SiC/SiC composites fabricated via reactive melt infiltration (RMI). The process began with the impregnation of SiC fiber cloth with phenolic resin, followed by lamination and pyrolysis. Subsequent steps included further impregnations with phenolic resin, SiC slurry, and carbon black slurry, each followed by additional pyrolysis.

Eco-Friendly Castor Oil-Based Composite with High Clam Shell Powder Content.

Eco-friendly castor oil-based composites with a high content of clam shell powder were prepared in this study. Biomass composites were prepared by blending castor-oil-based polyurethane prepolymer (COPU) with a filler consisting of high-content clam shell powder (CSP), named CSP-COPU. The structure, microstructure, mechanical properties, and thermal stability of the composites were investigated.

Conicity index and sex differences in relation to carotid plaque instability in Chinese community residents: Conicity index and carotid plaque.

Objectives: The conicity index (CI), an anthropometric parameter of visceral obesity, has exhibited a strong correlation with the incidence of atherosclerosis and cardiovascular events. This study aimed to explore the relationship between CI and carotid plaque instability, as well as the potential differences between sexes in this context.

Methods: Our cross-sectional study included a total of 44,248 participants from the China Stroke High-risk Population Screening and Intervention Program (CSHPSIP), all of whom underwent carotid ultrasound for the evaluation of carotid plaque.

Engineered Mesenchymal Stem Cell-Derived Extracellular Vesicles Scavenge Self-Antigens for Psoriasis Therapy via Modulating Metabolic and Immunological Disorders.

Psoriasis is a chronic inflammatory dermatosis driven by excessive activation of the immune system. Recent studies have demonstrated the therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) to psoriasis because of their immunomodulation functions. Yet, the outcome of MSC-EVs alone is still unsatisfactory and the underlying mechanisms are also unclear.

Single incision laparoscopic totally preperitoneal hernioplasty (SIL-TPP) for bilateral inguinal hernia repair: initial experience.

Objective: The aim of this study was to introduce and assess the safety and feasibility of single incision laparoscopic totally preperitoneal hernioplasty (SIL-TPP) for bilateral inguinal hernia repair.

Method: Forty-two SIL-TPP procedures for bilateral inguinal hernia repair were conducted from June 2018 to July 2022 at the First Affiliated Hospital of Ningbo University using standard laparoscopic instruments and a single-port device. Clinical data such as demographic intraoperative parameters and short-term postoperative outcomes were collected and analysed.

Label-free determination of glypican-3 using PtPd@H-rGO nanocomposites decorated light-addressable potentiometric sensor.

Glypican-3 (GPC3) is exclusively overexpressed in most Hepatocellular carcinoma (HCC) tissue but not in normal liver tissue, making it a promising biomarker for the precise detection of HCC. In this paper, a label-free light-addressable potentiometric sensor (LAPS) decorated by platinumpalladium-hemin-reduced graphene oxide nanocomposites (PtPd@H-rGO NCs) was constructed for determination of GPC3. The GPC3 aptamer (GPC3) and PtPd@H-rGO NCs were modified on the surface of silicon-based LAPS chip to build sensitive unit of LAPS system.

Novel Single Perovskite Material for Visible-Light Photocatalytic CO Reduction via Joint Experimental and DFT Study.

Developing advanced and economically viable technologies for the capture and utilization of carbon dioxide (CO) is crucial for sustainable energy production from fossil fuels. Converting CO into valuable chemicals and fuels is a promising approach to mitigate atmospheric CO levels. Among various methods, photocatalytic reduction stands out for its potential to reduce emissions and produce useful products.

Synthetic lignin derived from ferulic acid for UV-blocking sunscreen.

Conventional lignin, a dehydrogenated polymer derived primarily from hydroxycinnamic alcohol monomers, exhibits relatively low antioxidant and ultraviolet (UV)-blocking activities due to its structural imperfection. Herein, we demonstrated that the dehydrogenated polymer of ferulic acid (an unconventional lignin precursor) (FAL) had excellent antioxidant and UV-blocking properties. Structural characterization showed that FAL contains abundant dihydrobenzofuran and butyrolactone structures, free phenolic hydroxyl groups, and cinnamic acid end groups.

PCYT2 inhibits epithelial-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation.

Metabolic reprogramming is a common feature in tumor progression and metastasis. Like proteins, lipids can transduce signals through lipid-protein interactions. During tumor initiation and metastasis, dysregulation of the Hippo pathway plays a critical role.

Using Label-Free Raman Spectroscopy Integrated with Microfluidic Chips to Probe Ferroptosis Networks in Cells.

Ferroptosis, a regulated form of cell death driven by oxidative stress and lipid peroxidation, has emerged as a pivotal research focus with implications across various cellular contexts. In this study, we employed a multifaceted approach, integrating label-free Raman spectroscopy and microfluidics to study the mechanisms underpinning ferroptosis. Our investigations included the ferroptosis initiation based on the changes in the lipid Raman band at 1436 cm under different cellular states, the generation of reactive oxygen species (ROS), lipid peroxidation, DNA damage/repair, and mitochondrial dysfunction.

Early-stage Alzheimer's disease profiling in blood achieved by multiplexing aptamer-SERS biosensors.

Neurological disorders are the second leading cause of death globally, with Alzheimer's disease (AD) emerging as a significant contributor, responsible for 276 million cases in disability-adjusted life years. Conventional diagnostic methods are often invasive, costly, and place a considerable strain on global healthcare systems. In this study, we presented an innovative and efficient strategy for AD assessment through blood profiling using a multiwell glass chip integrated with aptamer-based surface-enhanced Raman scattering (SERS) biosensors.

A protein vaccine of RBD integrated with immune evasion mutation shows broad protection against SARS-CoV-2.

Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue to emerge and evade immunity, resulting in breakthrough infections in vaccinated populations. There is an urgent need for the development of vaccines with broad protective effects. In this study, we selected hotspot mutations in the receptor-binding domain (RBD) that contribute to immune escape properties and integrated them into the original RBD protein to obtain a complex RBD protein (cRBD), and we found cRBDs have broad protective effects against SARS-CoV-2 variants.

ROS-responsive Hydrogel Loaded with Allicin Suppresses Cell Apoptosis for the Treatment of Intervertebral Disc Degeneration in a Rat Model.

Background: Intervertebral disc degeneration (IVDD) is a common cause of lower back pain, and cell apoptosis plays a key role in its progression. This study explores the therapeutic potential of a reactive oxygen species (ROS)-responsive hydrogel loaded with allicin for treating IVDD.

Methods: Allicin was encapsulated in an ROS-responsive hydrogel, and its controlled release was studied in vitro.

Rapid Hemostasis Tumor In Situ Hydrogel Vaccines for Colorectal Cancer Chemo-Immunotherapy.

Due to the high heterogeneity and the immunosuppressive microenvironment of tumors, most single antigen tumor vaccines often fail to elicit potent antitumor immune responses in clinical trials, resulting in unsatisfactory therapy effects. Hence, personalized tumor vaccines have become a promising modality for cancer immunotherapy. Here, we have developed a tumor in situ hydrogel vaccine (AH/DA-OR) capable of rapid hemostasis for personalized tumor immunotherapy, composed of dopamine-grafted hyaluronic acid (HA/DA) combined with sodium alginate (ALG), with coloaded oxaliplatin (OXA) and resiquimod (R848).