Virtual screening and activity evaluation of multitargeting inhibitors for idiopathic pulmonary fibrosis.

Transforming growth factor β receptor (TGF-β1R) and receptor tyrosine kinases (RTKs), such as VEGFRs, PDGFRs and FGFRs are considered important therapeutic targets in blocking myofibroblast migration and activation of idiopathic pulmonary fibrosis (IPF). To screen and design innovative prodrug to simultaneously target these four classes of receptors, we proposed an approach based on network pharmacology combining virtual screening and machine learning activity prediction, followed by efficient and models to evaluate drug activity. We first constructed Collagen1A2-A549 cells with type I collagen as the main biomarker and evaluated the activity of compounds to inhibit collagen expression at the cellular level.

CoQ10 enhances the efficacy of airway basal stem cell transplantation on bleomycin-induced idiopathic pulmonary fibrosis in mice.

Background: Recent studies have demonstrated that airway basal stem cells (BCs) transplantation can ameliorate bleomycin-induced idiopathic pulmonary fibrosis (IPF) through lung regeneration promotion. However, BCs under oxidative stress in the alveolar microenvironment are poor in survival, causing unsatisfied efficacy of BCs transplantation. In this study, we investigated whether Coenzyme Q10(CoQ10) counteracts oxidative stress in the alveolar microenvironment, thus improved the efficacy of BCs transplantation for IPF treatment.

Surface Ligand Engineering for a Lead-Free CsCuBr Microcrystal-Based Humidity Sensor with a Giant Response.

Halide perovskites are potential humidity-detection materials due to their sensitivity to water, but the instability of traditional lead-based halide perovskites and the toxicity of Pb hinder further application in humidity sensing. Here, lead-free CsCuBr perovskite microcrystals passivated by surface ligands (OLA and OAm) are used to prepare an environmentally friendly humidity sensor. The humidity sensing performance of the prepared sensors was tested, and the effect of surface ligands of perovskites on the performance of humidity sensors was analyzed.

A rapid and simple HPLC-MS/MS method for the simultaneous quantification of valproic acid and its five metabolites in human plasma and application to study pharmacokinetic interaction in Chinese epilepsy patients.

Valproic acid(VPA) is a classic drug that used to treat epilepsy in monotherapy or combination with other anticonvulsant drugs such as lamotrigine (LTG). Although it was reported that VPA could increase lamotrigine trough concentration in clinical practice, there was no report about the effect of LTG on the trough concentration of VPA and its main metabolites, such as 4-ene-VPA, 3-OH-VPA, 4-OH-VPA, 3-keto-VPA, 2-PGA which are related to the therapeutic and toxic effects of VPA. In this study, a simple and rapid method for the simultaneous determination of VPA and its five metabolites in human plasma using HPLC-MS/MS was developed for the first time.

[Processing GC-FTIR by the blind source separation].

An analysis method for separating chromatographic overlapped peaks and purifying infrared spectra is put forward, based on the blind source separation technique and the multi-dimensional data of GC-FTIR, Using various information from hyphenated instruments, this method was used to separate completely a organic mixture, the xylene isomerism system, a problem unable to solve usually. The method can confirm the rationality of theory and algorithm and give integral explanations of the independent component analysis data. The reason for the error in quantitative analysis is discussed.

[Study on near-infrared absorption mechanism of alkali lignin].

The results showed that o-quinone and q-quinone had strong absorption while vanillin had nearly no absorption in the 800-900 nm range of near-infrared spectroscopy through the comparison of their near-infrared absorption spectra. It was proved that quinone structure of alkali lignin had strong absorption in the 800-900 nm range of near-infrared spectroscopy. The change in the absorbency of oleander milled wood lignin treated with NaOH and Na2 S before and after is greater than that in the absorbency of ginkgo milled wood lignin treated with NaOH and Na2 S before and after because more quinone structure was formed in the process of oleander milled wood lignin treated with NaOH and Na2 S.