Mediating effect analysis of visceral adiposity index on free triiodothyronine to free thyroxine ratio and non-alcoholic fatty liver disease in euthyroid population.

Background: The association between free triiodothyronine/free thyroxine (FT3/FT4) and non-alcoholic fatty liver disease (NAFLD) in euthyroid subjects is unclear. In addition, few studies have explored whether VAI mediates the association between FT3/FT4 ratio and NAFLD in the euthyroid population. We aimed to analyze the mediating effect of VAI on the FT3/FT4 ratio and NAFLD risk in the euthyroid population.

[Etiology and genetic diagnosis of short stature in children].

Objective: To study the etiology and genetic diagnosis of children with short stature.

Methods: A retrospective analysis was performed to study the etiological distribution and clinical features of 86 children with short stature.

Results: A total of 6 causes were observed in these children, among which idiopathic short stature (ISS, 41%) and growth hormone deficiency (GHD, 29%) were the most common causes, followed by genetic diseases (14%).

[Changes in Ca(2+)concentration and caspase-3 expression and their relationship in Raji cells exposed to electromagnetic radiation].

Objective: To study the effects of electromagnetic pulse (EMP), S-band high power microwave (S-HPM), and X-band high power microwave (X-HPM) on the Ca(2+) concentration and caspase-3 expression in Raji cells and the relationship between Ca(2+) concentration and caspase-3 expression, and to investigate the regulatory mechanism of electromagnetic radiation damage.

Methods: Raji cells were cultured conventionally. Some cells were irradiated by EMP, S-HPM, and X-HPM in the logarithmic growth phase for 6 hours and then collected; others received sham irradiation as a control.

A novel metallo-organically templated pentaborate: acetato[N,N'-bis(2-aminoethyl)ethane-1,2-diamine]zinc(II) 4,4',6,6'-tetrahydroxy-2,2'-spirobi[cyclotriboroxane](1-).

The title compound, [Zn(C2H3O2)(C6H18N4)][B5O6(OH)4], contains mixed-ligand [Zn(CH3COO)(teta)]+ complex cations (teta is triethylenetetramine) and pentaborate [B5O6(OH)4]- anions. The [B5O6(OH)4]- anions are connected to one another through hydrogen bonds, forming a three-dimensional supramolecular network, in which the [Zn(CH3COO)(teta)]+ cations are located.

Pro-protein convertase-2/carboxypeptidase-E mediated neuropeptide processing of RGC-5 cell after in vitro ischemia.

[This retracts the article DOI: 10.1007/s12264-009-1027-8.].

PC2/CPE-mediated pro-protein processing in tumor cells and its differentiated cells or tissues.

Pro-protein convertase-2 (PC2) and carboxypeptidase-E (CPE) proteins are two major members of the pro-protein convertases that involve in the maturation of protein precursor. By using PC2 activity, immunocytochemistry (ICC) and Western blot method, PC2, CPE and preproNPY protein expression levels were compared among mature retina tissue, RGC-5 cells and its differentiated cells, or brain cortex tissue, NS20Y tumor cells and its differentiated cells, or mature breast tissue, breast tumor cell RM1 and breast adenocarcinoma tissue. The experimental results indicated that the differentiated cells or tissues had higher or highest PC2 activity.

Pro-protein convertase-2/carboxypeptidase-E mediated neuropeptide processing of RGC-5 cell after in vitro ischemia.

Objective: To observe the change of the neuropeptide pro-protein processing system in the ischemic retina ganglion cell-5 (RGC-5) cells, pro-protein convertase-2 (PC2), carboxypeptidase-E (CPE) and preproneuropeptide Y (preproNPY) protein levels in the ischemic RGC-5 cells and conditioned medium were analyzed.

Methods: The RGC-5 cell was differentiated in 0.1 mumol/L staurosporine for 24 h and then stressed by different doses of oxygen and glucose deprivation (OGD).

Investigation of Epstein-Barr virus in Chinese colorectal tumors.

Aim: To elucidate the association of Epstein-Barr virus (EBV) with colorectal tumors and to demonstrate whether infection of EBV existed in different stages of colorectal tumors involves in the carcinogenesis.

Methods: One hundred and thirty paraffin-embedded tissues of colorectal tumors were classified into 5 groups: 26 adenomas, 23 adenomas complicated with dysplasia, 22 adenomas complicated with carcinomatous, 36 colon carcinoma and 23 HNPCC, were examined by PCR, IHC and ISH, respectively.

Results: EBV DNA was detected by PCR in 26 cases out of the 130 specimens, including 5 cases of adenomas, 5 adenomas complicated with dysplasia, 5 adenomas complicated with carcinomatous, 7 colorectal carcinoma and 4 HNPCC.

Detection of Epstein-Barr virus in human colorectal cancer by in situ hybridazition.

Objective: To investigate the relationship between Epstein-Barr virus (EBV) and the pathogenesis of human colorectal cancer.

Methods: The small-molecule RNA fragments of EBV was examined by in situ hybridization in colorectal cancer specimens obtained from 130 patients.

Results: EBV was found in the 6 of 130 colorectal cancer cases.