[Comparison of different isolation methods on adult Sertoli cells co-transplanted with islets].

Aim: To establish a more convenient and effective method for isolating adult Sertoli cells and apply the method to islet transplantation.

Methods: Trypsin, DNase, collagenase and hyaluronidase were used for a one-step digestion in group A. A two-step digestion was used in group B, in which testis tissues were first digested by trypsin and DNase and then were digested by collagenase and hyaluronidase.

Study on systemic immune tolerance induction in rat islet transplantation by intravenous infusion of Sertoli cells.

Background: Sertoli cells are usually co-transplanted with pancreatic islets to induce local immune tolerance. In this report, we used infusion with Sertoli cells in islet transplantation to induce systemic immune tolerance and studied the mechanism of the tolerance induction.

Methods: Streptozotocin-induced diabetic rats were divided into four groups before islet transplantation: group A as control; group B with intravenous infusion of Sertoli cells; group C with Sertoli cell infusion and Fas ligand antibody treatment; and group D with Sertoli cell infusion and transforming growth factor-beta1 antibody treatment.

Application of kushenin on patients with chronic hepatitis C after renal transplantation.

Objective: To evaluate the efficacy of kushenin in treating patients with chronic hepatitis C after renal transplantation.

Methods: Fifty-five patients were randomly assigned by lottery to the treatment group (29 cases) and control group (26 cases). The same immunosuppression therapy was given to all patients in both groups.