[Analysis of Patients' Irregular Antibody Screening and Identification Results before Blood Transfusion].

Objective: To analyze the results of irregular antibody screening and identification among patients before blood transfusion, and to investigate the specific distribution of irregular antibodies and the distribution regularity in different diseases.

Methods: Choosing the patients intended to be transfused in our hospital from January 1, 2009 to December 31, 2013 years, micro-column gel technique was used to screen the irregular antibodies of those receptors and to identify the antibody specificity of the positive specimens.

Results: Among 44194 patients, 137 patients were with irregular antibody positive and their positive rate was 0.

[Analysis of the Physiological Activities and Functions in Vitro of Apheresis Platelets during Storage].

Objective: To investigate the changes of physiological activities and functions in vitro of apheresis platelets during storage.

Method: 17 units of apheresis platelets were randomly chosen and stored at 20 °C to 24 °C with agitation. Platelet counting (Plt), mean platelet volume (MPV), blood gases, pH value, glucose (Glu) concentration, lactate (LA) concentration, LDH concentration, thromboelastogram (TEG), hypotonic shock response (HSR), CD62p expression rate and anew expression rate were measured on days 0, 1, 3, 5 after platelet storage.

[Role of antioxidant in protecting the biological function of hematopoietic stem cells].

In peripheral blood hematopoietic stem cell transplantation (PBHSCT) , the mobilization and circulating of bone marrow hematopoietic stem cells in blood with higher oxygen concentration all increase reactive oxygen species(ROS) production, which has negative effect on the biological function of BMHSC. In order to investigate the protective effect of antioxidant on hematopoietic stem cells (HSC), the ascorbic acid 2-phosphate (AA2P), an ascorbic acid derivative of vitamin C, was added in HSC culturing by imitating oxygen conditions which BMHSC experienced in peripheral blood stem cell transplantation. The protective effect of above-mentioned culture methods on the biologic functions of BMHSC was evaluated by vitro amplification assay, committed division assay, reactive oxygen species (ROS) measurement, CD34(+) HSC engraftment.

[Influence of reactive oxygen species on mouse bone marrow hematopoietic stem cell apoptosis].

Objective of this study was to investigate the mechanism of the biological function damage resulting from increased ROS in peripheral blood stem cells during peripheral blood stem cell transplantation. Bone marrow hematopoietic stem cells (BMHSC) were cultured at the oxygen concentration imitated according to the bone marrow oxygen concentration (5% O2) including mean venous oxygen concentration (12% O2), mean arterial oxygen concentration (20% O2). The ROS level in BMHSC was detected by using fluorescent probe, the percentage of BM-HSC in cell cycle was determined by flow cytometry, the apoptosis rate was assayed by Annexin V/PI double staining, the expression levels of ATM gene and P21 protein were measured by PCR and Western respectively.

[Correlation between reactive oxygen species of cryopreserved peripheral blood mononuclear cells and expression of homing adhesion molecules on peripheral blood hematopoietic stem/progenitor cells].

This study was purposed to detect the level of reactive oxygen species (ROS) in cryopreserved peripheral blood mononuclear cells (PBMNC) and the expression of homing molecules on peripheral blood hematopoietic stem/progenitor cells, and to analyze the correlation between ROS level and the expression of homing molecules. The survival percentage of PBMNC was determined by trypan blue exclusion rate. The expression of antigens on peripheral blood hematopoietic stem/progenitor cells, such as CD34, CD133 and some related homing molecules (VLA4, CXCR4 and CD44) were detected by flow cytometry.