[Isolation and characterization of the first strain of Helicobacter hepaticus in China].

Helicobacter hepaticus is nongastric helicobacter that can reside in the hepatobiliary and intestinal systems of many animal hosts, leading to proliferative hepatitis, hepatocellular carcinoma, typhlitis, and colonitis. In this study, the intestinal mucosa was isolated from BALB/c mice to prepare tissue homogenate and spread onto selective C jejuni blood agar plates for incubation in the presence of trimethoprim, vancomycin, and polymyxin at 37 degrees Celsius; under microaerobic conditions in vented jars containing 5% O2, 10%CO2, and 85% N2. The bacteria were identified morphologically and biochemically.

[Immunization with catalase and UreB two-valence vaccine for preventing Helicobacter pylori infection in mice].

Objective: To investigate the effects of the two-valence vaccine consisting of Helicobacter pylori (H. pylori) catalase and urease subunit UreB in preventing H. pyloriinfection in mice.

Pathogenicty and immune prophylaxis of cag pathogenicity island gene knockout homogenic mutants.

Aim: To clarify the role of cag pathogenicity island (cagPAI) of Helicobacter pylori (H pylori ) in the pathogenicity and immune prophylaxis of H pylori infection.

Methods: Three pairs of H pylori including 3 strains of cagPAI positive wildtype bacteria and their cagPAI knockout homogenic mutants were utilized. H pylori binding to the gastric epithelial cells was analyzed by flow cytometry assays.

[Regulation of Fas expression in gastric epithelium: one of the auto-immune mechanisms of Helicobacter pylori pathogenesis].

Objective: To investigate the pathogenic mechanism of Helicobacter pylori (H.pylori)-mediated gastric epithelial cell damage.

Methods: Fas expressions in gastric epithelial cell lines and freshly isolated gastric epithelial cells with or without H.

[In vitro evaluation of the safety and biological activity of recombinant Helicobacter pylori blood group antigen-binding adhesin].

Objective: To evaluate the safety and biological activity of recombinant Helicobacter pylori (Hp) blood group antigen- binding adhesin (rBabA ) in vitro so as to investigate the feasibility of using rBabA as a Hp vaccine.

Methods: ELISA was used to measure rBabA-specific antibody in the serum of Hp-infected patients, and the proliferation of T lymphocytes in response to rBabA was examined by MTT assay. T cell apoptosis induced by rBabA was detected by diphenylamine assay.

Conservative region of the genes encoding four adhesins of Helicobacter pylori: cloning, sequence analysis and biological information analysis.

Objective: To clone the conserved regions of the genes encoding the 4 adhesins (BabA, AlpA, AlpB and HopZ) of Helicobacter pylori (H. pylori) and analyze their sequences and biological information, thus facilitating further research in the molecular mechanism and immunogenicity of H. pylori adhesins.