Study on the Regulatory Mechanisms of Carapace Marking Formation in .

There are two phenotypes in the natural populations of , which is an ideal model for studying the formation of markings and body color in crustaceans. In a previous study, we used comparative transcriptome technology to screen some functional genes related to body color regulation. Here, high-throughput sequencing technology was used to perform microRNA (miRNA) sequencing analysis on the exoskeleton of with two types of carapace markings, and functional studies of related genes were performed.

Parameter-dependent cell-type specific effects of transcranial focused ultrasound stimulation in an awake head-fixed rodent model.

Transcranial focused ultrasound (tFUS) is a promising neuromodulation technique able to target shallow and deep brain structures with high precision. Previous studies have demonstrated that tFUS stimulation responses are cell-type specific, specifically tFUS can elicit time-locked neural activity in regular spiking units (RSUs) that is sensitive to increases in pulse repetition frequency (PRF), while time-locked responses are not seen in fast spiking units (FSUs). These findings suggest a unique capability of tFUS to alter circuit network dynamics with cell-type specificity; however, these results could be biased by the use of anesthesia, which significantly modulates neural activities.

Effects of Exposure on : Hepatopancreas Histology, Antioxidant Enzyme Activity, and Transcriptome Analysis.

, a dinoflagellate known for causing harmful algal blooms (HABs), has garnered significant attention due to its potential toxicity to marine ecosystems, fisheries, and human health. However, the effects of this toxin-producing alga on shrimp are not yet comprehensively understood. This study aimed to assess the hepatopancreas damage induced by in the economically important shrimp species and to elucidate the underlying molecular mechanisms through histology, antioxidant enzyme activity, and transcriptome analysis.

The novel ginseng Rh2 derivative 2-deoxy-Rh2, exhibits potent anticancer effect via the AMPK/mTOR/autophagy signaling pathway against breast cancer.

Breast cancer is the most prevalent cancer and the second leading cause of cancer-related mortality among women globally, resulting in considerable psychological and physical distress for patients. Our previous study synthesized a novel derivative, 2-Deoxy-Rh2, which exhibited anticancer properties by influencing glycolysis and mitochondrial respiration. The objective of the current study was to investigate the anti-proliferative effects and underlying mechanisms of 2-Deoxy-Rh2 on human breast cancer cell lines MCF-7 and MDA-MB-231.

Significant expansion of small water bodies in the Dongting Lake region following the impoundment of the Three Gorges Dam.

Small water bodies (SWBs) are vital for freshwater biodiversity and ecosystem services, yet they remain underrepresented in research compared to larger water bodies, despite being particularly vulnerable to anthropogenic activities and climate change. Advances in satellite remote sensing, particularly the Joint Research Centre's Global Surface Water (JRC-GSW) dataset derived from Landsat imagery, provide an unprecedented opportunity for high-resolution and long-term analysis of SWBs spatio-temporal dynamics. This study leverages the JRC-GSW dataset to assess the impacts of the Three Gorges Dam (TGD) on SWBs in the Dongting Lake region, focusing on maximum and minimum water extents during wet and dry seasons pre- and post-dam impoundment.

Co-exposure to F-53B and nanoplastics induced hepatic glucolipid metabolism disorders by the PI3K-AKT signaling pathway.

Recent investigations suggest that the chemical compound F-53B (6:2 chlorinated polyfluorinated ether sulfonate) may pose risks of liver toxicity. Within environmental settings, F-53B attaches to microplastics and nanoplastics, which are capable of being consumed by diverse species. To investigate the synergistic effects on hepatotoxicity, adult male mice were subjected to F-53B at daily doses of 1, 10, and 100 μg/kg, NPs at 100 mg/kg per day, or a combination of both treatments for a duration of 2 months.

Co-Delivery of Dacarbazine and miRNA 34a Combinations to Synergistically Improve Malignant Melanoma Treatments.

Purpose: The incidence of malignant melanoma (MM) has risen over the past three decades, and despite advancements in treatment, there is still a need to improve treatment modalities. This study developed a promising strategy for tumor-targeted co-delivery of Dacarbazine (DTIC) and miRNA 34a-loaded PHRD micelles (Co-PHRD) for combination treatment of MM.

Methods: To construct the dual drug-loaded delivery system Co-PHRD, poly (L-arginine)-poly (L-histidine)-polylactic acid (PLA) was employed as a building block.

Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes.

The present study was conducted to analyze the correlation between the milk fat content of Binglangjiang buffaloes and their microbial and host metabolites. The 10 buffaloes with the highest milk fat content (HF, 5.60 ± 0.

Discovery of phloridzin as a new antagonist for Di(2-ethylhexyl) phthalate-induced male reproductive toxicity based on the adverse outcome pathway network and drug-target gene set enrichment analysis.

Di(2-ethylhexyl) phthalate (DEHP) is a widespread ubiquitous phthalate environmental contaminant. The male reproductive toxicity (MRT) from exposure to DEHP and its main metabolite, mono(2-ethylhexyl) phthalate (MEHP), has been well documented. Fully elucidating its toxic mechanism and discovering effective antagonists are desirable means to reduce the health risks of DEHP.

Oncogenic role of RARG rearrangements in acute myeloid leukemia resembling acute promyelocytic leukemia.

Acute myeloid leukemia (AML) featuring retinoic acid receptor-gamma (RARG) rearrangements exhibits morphological features resembling those of acute promyelocytic leukemia but is associated with drug resistance and poor clinical outcomes. However, the mechanisms underlying the role of RARG fusions in leukemogenesis remain elusive. Here, we show that RARG fusions disrupt myeloid differentiation and promote proliferation and self-renewal of hematopoietic stem and progenitor cells (HSPCs) by upregulating BCL2 and ATF3.

Effects of pristine and photoaged tire wear particles and their leachable additives on key nitrogen removal processes and nitrous oxide accumulation in estuarine sediments.

Despite growing attention to the environmental pollution caused by tire wear particles (TWPs), the effects of pristine and photoaged TWPs (P-TWPs and A-TWPs) and their TWP leachates (TWPLs; P-TWPL and A-TWPL) on key nitrogen removal processes in estuarine sediments remain unclear. This study explores the responses of the denitrification rate, anammox rate, and nitrous oxide (NO) accumulation to P-TWP, A-TWP, P-TWPL, and A-TWPL exposure in estuarine sediments, and assesses the potential biotoxic substances present in TWPLs. P-TWPs reduced the denitrification rate by 17.

A Model for Selecting Kiwifruit () Germplasm Resources with Excellent Fruit Quality.

The evaluation of quality traits is an important procedure for kiwifruit breeding and comprehensive utilization. The present study aimed to establish a comprehensive system to assess germplasms by analyzing 22 quality traits on kiwifruit samples collected from a wild population of 236 plants grown in the Jiangxi Province, China. Variability, correlation, principal components, and cluster analyses were carried out using the data collected from fruit quality evaluations.

Genome-wide CRISPR-based screen identifies E2F transcription factor 1 as a regulator and therapeutic target of aristolochic acid-induced nephrotoxicity.

Aristolochic Acid I (AAI) is widely present in traditional Chinese medicines derived from the Aristolochia genus and is known to cause significant damage to renal tubular epithelial cells. Genome-wide screening has proven to be a powerful tool in identifying critical genes associated with the toxicity of exogenous substances. To identify undiscovered key genes involved in AAI-induced renal toxicity, a genome-wide CRISPR library screen was conducted in the human kidney-2 (HK-2) cell line.

Phenomics-assisted genetic dissection and molecular design of drought resistance in rice.

Dissecting the mechanism of drought resistance (DR) and designing drought-resistant rice varieties are promising strategies to address the challenge of climate change. Here, we selected a typical drought-avoidant (DA) variety, IRAT109, and a drought-tolerant (DT) variety, Hanhui15, as parents to develop a stable recombinant inbred line (RIL) population (F, 1262 lines). The de novo assembled genomes of both parents were released.

Regulation of Milk Fat Synthesis: Key Genes and Microbial Functions.

Milk is rich in a variety of essential nutrients, including fats, proteins, and trace elements that are important for human health. In particular, milk fat has an alleviating effect on diseases such as heart disease and diabetes. Fatty acids, the basic units of milk fat, play an important role in many biological reactions in the body, including the involvement of glycerophospholipids and sphingolipids in the formation of cell membranes.

A deep LSTM-based constitutive model for describing the impact characteristics of concrete-granite composites with different roughness interfaces.

In this work, the dynamic mechanical properties of concrete‒granite composites with various roughness interfaces were investigated via split Hopkinson pressure bar (SHPB) system to evaluate the impact resistance of the lining‒surrounding rock composite structure that is commonly present in rock engineering. The dynamic uniaxial compressive strength of the composite at an impact speed of 11.3 m/s may increase by 20.

The alpha2-adrenoceptor agonist clonidine protects against hypoxic-ischemic brain damage in neonatal mice through the Nrf2/NF-κB signaling pathway.

Neonatal hypoxic-ischemic brain damage (HIBD) is a severe condition closely associated with neuroinflammation and oxidative stress. Clonidine, a selective α2-adrenergic receptor agonist, is known for its anti-inflammatory and antioxidant properties. Despite these recognized therapeutic benefits, the exact mechanisms by which clonidine exerts its effects in the context of HIBD are not fully understood.

Activation and antitumor immunity of CD8 T cells are supported by the glucose transporter GLUT10 and disrupted by lactic acid.

CD8 T cell activation leads to the rapid proliferation and differentiation of effector T cells (T), which mediate antitumor immunity. Although aerobic glycolysis is preferentially activated in CD8 T, the mechanisms that regulate CD8 T cell glucose uptake in the low-glucose and acidic tumor microenvironment (TME) remain poorly understood. Here, we report that the abundance of the glucose transporter GLUT10 is increased during CD8 T cell activation and antitumor immunity.

Identification of sanguinarine as a novel antagonist for perfluorooctanoate/perfluorooctane sulfonate-induced senescence of hepatocytes: An integrated computational and experimental analysis.

Perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS), two prominent per- and polyfluoroalkyl substances (PFASs), are potentially harmful to many human organs. However, there only exist limited methods to mitigate their health hazards. The aim of this study is to combine a bioinformatics analysis with in vitro experiments to discover small molecules that can alleviate liver damage caused by PFOA/PFOS.

Exploring the Potential Mechanisms of Zhu-Ye-Qing Wine in Preventing Cardiovascular and Cerebrovascular Diseases Based on Network Pharmacology.

Cardiovascular and cerebrovascular disease (CCVD) is a complex disease with a long latency period, and the most effective diagnosis and treatment methods are risk assessment and preventive interventions before onset. According to traditional Chinese medicine (TCM), Zhu-Ye-Qing wine (ZYQW) has the effect of invigorating blood and removing blood stasis. However, whether ZYQW can improve the progression of CCVD has not been reported.

Enhancement of Biological Nitrogen Removal System Resilience to Chronic Exposure of Zinc Oxide Nanoparticles by Quorum Sensing Modulation: Physiochemical, Microbial, and Metabolic Insights.

The effects of three typical N-acyl-homoserine lactones (AHLs) on the tolerance of biological nitrogen removal (BNR) system to chronic exposure of zinc oxide nanoparticles (NPs) were investigated. C-HSL successfully delayed the crash time of nitrogen removal performances in the NP-stressed system, while C-HSL and C-HSL maintained total nitrogen removal efficiencies throughout the 90-day NP exposure. All three AHLs increased NPs' contents captured in extracellular polymeric substances, alleviating membrane damage and preserving floc structure.

Transcranial focused ultrasound activates feedforward and feedback cortico-thalamo-cortical pathways by selectively activating excitatory neurons.

Transcranial focused ultrasound stimulation (tFUS) has been proven capable of altering focal neuronal activities and neural circuits non-invasively in both animals and humans. The abilities of tFUS for cell-type selection within the targeted area like somatosensory cortex have been shown to be parameter related. However, how neuronal subpopulations across neural pathways are affected, for example how tFUS affected neuronal connections between brain areas remains unclear.