A New Diterpenoid Alkaloid with Antimicrobial Activity from Aconitum brachypodum Diels.

A new C-denudatine-type diterpenoid alkaloid (DA) 11S-aconicarnine D (1) and fifteen known DAs were isolated from the lateral roots of Aconitum brachypodum Diels. Their structures were identified on the basis of extensive spectroscopic analyses, NMR calculations and DP4+ analysis. Compounds 1 and 4 exhibited antimicrobial activity against Alternaria panax with MICs of 2.

Thalamocortical dysconnectivity is associated with pain in patients with knee osteoarthritis.

Previous studies have suggested that the morphology and function of the thalamus and cortex are abnormal in patients with knee osteoarthritis (KOA). However, whether the thalamocortical network is differentially affected in this disorder is unknown. In this study, we examined functional and effective connectivity between the thalamus and major divisions of the cortex in 27 healthy controls and 27 KOA patients using functional magnetic resonance imaging.

Disrupted Resting-State Functional Connectivity and Effective Connectivity of the Nucleus Accumbens in Chronic Low Back Pain: A Cross-Sectional Study.

Purpose: Chronic low back pain (cLBP) is a recurring and intractable disease that is often accompanied by emotional and cognitive disorders such as depression and anxiety. The nucleus accumbens (NAc) plays an important role in mediating emotional and cognitive processes and analgesia. This study investigated the resting-state functional connectivity (rsFC) and effective connectivity (EC) of NAc and its subregions in cLBP.

A new C-diterpenoid alkaloid in Comber.

Nine diterpenoid alkaloids were isolated from Comber belonging to the genus in Ranunculaceae family. Their structures were determinated by using HR-ESI-MS and 1 D/2D NMR spectra as geordine (), yunaconitine (), chasmanine (), crassicauline A (), forestine (), pseudaconine (), 14-acetylalatisamine (), austroconitine B (), and talatisamine (). Among them, compound is a previously undescribed aconitine-type C-diterpenoid alkaloid, and compounds , and - have not previously been isolated from this species.

[Discharge and Contents of Exosome from Leukocyte-Reduced Apheresis Platelet Stored for Different Storage Time].

Objective: To explore the effect of storage time on discharge and content of exosome from leukocyte-reduced apheresis platelets (LRA-Plt).

Methods: Exosome (EXO) from LRA-Plt were acquired by ExoQuick, and its' morphology, immunological marker and particle size distribution were detected by transmission electron microscopy (TEM), Western blotting and dynamic light scattering (DLS), respectively. The changes in particle size distribution of EXO from LRA-Plt with different storage time were detected by DLS.

GPNMB silencing suppresses the proliferation and metastasis of osteosarcoma cells by blocking the PI3K/Akt/mTOR signaling pathway.

Glycoprotein non‑metastatic melanoma protein B (GPNMB) is a glycoprotein that is highly expressed in various types of cancer, including osteosarcoma. However, its cellular functions and related mechanisms in osteosarcoma remain unclear. In the present study, a higher GPNMB mRNA level was observed in osteosarcoma tissues, than in adjacent non‑cancerous tissues.

[Brain gray matter abnormalities revealed by voxel-based morphometry in patients with chronic low back pain].

Objective: To explore the morphometric abnormalities of brain gray matter (GM) in patients with chronic low back pain (CLBP).

Methods: Thirty patients with CLBP and 30 healthy individuals were enrolled and examined with a 3.0 T magnetic resonance (MR) scanner.

[Subcellular distribution of trace elements in wound granulation tissue of severe burn patients by inductively coupled plasma mass spectrometry].

A method for simultaneous and quantitative determination of Cr, Mn, Fe, Co, Cu, Zn, Se and Cd elements in the subcellular fractions of nuclei, mitochondria, lysosome, microsome and cytosol of wound granulation tissue of severe burn patients by octopole reaction system (ORS) inductively coupled plasma mass spectrometry (ICP-MS) was established. Using differential centrifugation, the sample is separated into different subcellular fractions. The subcellular fraction was digested by HNO3 + H2O2 with microwave digestion followed by dilution with ultrapure water then the above 8 trace elements in the solution were analyzed directly by ICP-MS.