Diterpenoids from Acanthopanax brachypus Harms.

Four diterpenoids, including two new ones, namely noracanthopanin A (1) and acanthopanolide B (2), and two known ones, ent-pimara-9(11),15-dien-19-oic acid (3) and ent-kaur-16-en-19-oic acid (4), were isolated from the stem bark of Acanthopanax brachypus Harms. Their structures were elucidated by physicochemical and spectroscopic analyses. Compound 1 and 2 exhibited moderate cytotoxicities against HO-8910 cells with IC50 values of 65.

Study on gas chromatography-mass spectrometry fingerprint of Acanthopanax brachypus.

As a peculiar folk medicinal plant, Acanthopanax brachypus was widely used to treat various diseases in China. At present, however, there is not a good quality standard for its quality evaluation. In this study, on the basis of the validation tests of precision, stability and repeatability, the chromatographic fingerprint of A.

Chemical composition, antimicrobial, antioxidant and cytotoxic activity of the essential oil from the leaves of Acanthopanax leucorrhizus (Oliv.) Harms.

The leaf essential oil of Acanthopanax leucorrhizus, a widely used medicinal plant, was obtained by hydrodistillation and analyzed by using combination of capillary GC-FID, GC-MS and RI. Fifty-nine components, representing 93.1% of the total oil, were identified in the essential oil and the main components of the oil were β-pinene (7.

Triterpenoid saponins from the stem bark of Acanthopanax brachypus HARMS.

Two new triterpenoid saponins, brachyposide A (1) {3-O-beta-D-galactopyranosyl-(1-->2)-[beta-D-xylopyranosyl-(1-->3)]-beta-D-glucuronopyranosyl-2beta,3beta,16alpha,23-tetrahydroxyolean-12-en-28-oic acid 28-O-beta-D-apiofuranosyl-(1-->3)-beta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosyl ester} and brachyposide B (2) {3-O-beta-D-glucopyranosyl-(1-->3)-beta-D-galactopyranosyl-(1-->3)-beta-D-glucuronopyranosyl-2beta,3beta,23-trihydroxyolean-12-en-28-oic acid 28-O-beta-D-xylopyranosyl-(1-->4)-[beta-D-apiofuranosyl-(1-->3)]-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranosyl ester}, together with four known triterpenoid saponins, including tabguticoside A, nipponoside D, palmatoside E and ciwujianoside A(1), were isolated from the stem bark of Acanthopanax brachypus. Their structures were elucidated on the basis of spectroscopic and chemical evidence.

Chemical compositions and antimicrobial activities of essential oils extracted from Acanthopanax brachypus.

The essential oils from the flowers, leaves, barks, roots and fruits of A. brachypus were individually extracted by hydrodistillation, and their chemical constituents were isolated and characterized by means of gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 160 different constituents accounting for 86.

[Study on chemical constituents and antimicrobial activity of the essential oil from Acanthopanax brachypus].

Objective: The chemical constituents and antimicrobial activity of the essential oil from Acanthopanax brachypus were studied.

Methods: The essential oil was extracted from the stem of A. brachypus by steam distillation, and its antimicrobial activity was tested in vitro.

Interaction of curcumin with intravenous immunoglobulin: a fluorescence quenching and Fourier transformation infrared spectroscopy study.

The interaction of curcumin with intravenous immunoglobulin (IVIG) mainly composed of immune gamma globulin (IgG) was studied in vitro by spectroscopic methods including fluorescence spectroscopy and Fourier transformation infrared (FTIR) spectroscopy. Docking was used to calculate the interaction mode between curcumin and IVIG. The binding parameters for the reaction were calculated according to the Sips equation, which suggested that the binding of IVIG to curcumin was characterized by two binding sites with the average affinity constant K(o) at 1.

Study on the interactions of kaempferol and quercetin with intravenous immunoglobulin by fluorescence quenching, fourier transformation infrared spectroscopy and circular dichroism spectroscopy.

The interactions of kaempferol and quercetin with intravenous immunoglobulin (IVIG) were studied in vitro by spectroscopic methods including fluorescence spectra, Fourier transformation infrared (FT-IR) spectra and circular dichroism (CD) spectra. The binding parameters for the reactions calculated according to the Sips equation suggested that the bindings of IVIG to kaempferol and quercetin were characterized by two binding sites with the average affinity constants K(o) at 1.032 x 10(4) M(-1) and 1.

Interaction of Taxol with intravenous immunoglobulin: an inhibition of Taxol from crystallizing in aqueous solution.

The interaction of anti-cancer drug Taxol (paclitaxel) with human intravenous immunoglobulin (IVIG) was studied under simulated physiological conditions in vitro by spectroscopic methods including fluorescence spectra and Fourier transformation infrared (FT-IR) difference spectra. Docking was used to calculate the interaction modes between Taxol with IVIG. Transmission electron microscopy (TEM) experiments were carried out to investigate the formation of aggregate and crystal of Taxol in aqueous solution.