Publications by authors named "Huaijun Si"

Protein ubiquitination is an important regulatory mechanism for biological growth and development against environmental influences, and can affect several biological processes, including the growth, development, and stress responses of plants. However, the function of potato-related ubiquitin-conjugating enzymes in abiotic stress tolerance is poorly understood. In this study, a with a UBC conserved structural domain was identified in potato and its function was investigated under osmotic stress and salt stress conditions.

View Article and Find Full Text PDF

The ubiquitin-proteasome system (UPS) is a key protein degradation pathway in eukaryotes, in which E3 ubiquitin ligases mediate protein ubiquitination, directly or indirectly targeting substrate proteins to regulate various biological processes, including plant growth, hormone signaling, immune responses, and adaptation to abiotic stress. In this study, we identified plant U-box protein 51 in () as an E3 ubiquitin ligase through transcriptomic analysis, and used it as a candidate gene for gene-function analysis. Quantitative real-time PCR (qRT-PCR) was used to examine expression across different tissues, and its expression patterns under simulated drought stress induced by polyethylene glycol (PEG 6000) were assessed.

View Article and Find Full Text PDF
Article Synopsis
  • In experiments, potato plants were subjected to salinity and osmotic stress using NaCl and PEG, leading to an analysis of the StGATA family genes via qRT-PCR and the establishment of modified plants with varying gene expression.
  • Results showed that overexpressing StGATA12 enhanced photosynthesis, transpiration, and stress resistance by regulating biochemical responses and gene activity, indicating its potential for improving potato plant resilience to environmental stresses.
View Article and Find Full Text PDF

The growth hormone export PIN-FORMED (PIN) is an important carrier for regulating the polar transport of plant growth hormones and plays an important role in plant growth and development. However, little is known about the characteristics and functions of PINs in potatoes. In this study, 10 members were identified from potatoes and named , , , , , , , , , and according to their positions in the potato chromosome In addition, the expression of 10 was analyzed by qRT-PCR during potato root development.

View Article and Find Full Text PDF
Article Synopsis
  • * In this study, 29 StATGs were identified and analyzed in potato, revealing their differential expression in various plant tissues and a response to drought and salt stresses.
  • * Overexpressing StATG8a in potato led to significantly improved tolerance to drought and salt, while knockdown lines showed decreased tolerance, highlighting the potential of StATG8a in stress management.
View Article and Find Full Text PDF

TCP transcription factors are involved in the regulation of plant growth and development and response to stress. Previous studies showed that was involved in the abiotic stress response of potato and positively regulated plant tolerance to drought stress. On the basis of previous studies, this study verified the downstream target genes of transcription factor binding through yeast one hybridization, double luciferase and other technologies, and conducted a preliminary analysis of the downstream target genes.

View Article and Find Full Text PDF

Autophagy is a highly conserved process in eukaryotes that is used to recycle the cellular components from the cytoplasm. It plays a crucial function in responding to both biotic and abiotic stress, as well as in the growth and development of plants. Autophagy-related genes (ATG) and their functions have been identified in numerous crop species.

View Article and Find Full Text PDF

Potato ( L.) is a major global food crop, and oxidative stress can significantly impact its growth. Previous studies have shown that its resistance to oxidative stress is mainly related to transcription factors, post-translational modifications, and antioxidant enzymes in vivo, but the specific molecular mechanisms remain unclear.

View Article and Find Full Text PDF

Backgrounds: As a conserved signaling pathway, mitogen-activated protein kinase (MAPK) cascade regulates cellular signaling in response to abiotic stress. High temperature may contribute to a significant decrease in economic yield. However, research into the expression patterns of family genes under high temperature is limited and lacks experimental validation regarding their role in supporting potato plant growth.

View Article and Find Full Text PDF

Plants often experience abiotic stress, which severely affects their growth. With the advent of global warming, drought stress has become a pivotal factor affecting crop yield and quality. Increasing numbers of studies have focused on elucidating the molecular mechanisms underlying plant responses to drought stress.

View Article and Find Full Text PDF

All over the world, potato (Solanum tuberosum L.) production is constrained by several biotic and abiotic factors. Many techniques and mechanisms have been used to overcome these hurdles and increase food for the rising population.

View Article and Find Full Text PDF

MAPKKs, as one of the main members of the mitogen-activated protein kinase (MAPK) cascade pathway, are located in the middle of the cascade and are involved in many physiological processes of plant growth and development, as well as stress tolerance. Previous studies have found that is responsive to drought and salt stress. To further investigate the function and regulatory mechanism of in potato stress response, potato variety 'Atlantic' was subjected to drought and NaCl treatments, and the expression of the gene was detected by qRT-PCR.

View Article and Find Full Text PDF

DNA-binding with one finger (Dof) proteins comprise a large family that play central roles in stress tolerance by regulating the expression of stress-responsive genes via the DOFCORE element or by interacting with other regulatory proteins. Although the Dof TF has been identified in a variety of species, its systemic analysis in potato ( L.) is lacking and its potential role in abiotic stress responses remains unclear.

View Article and Find Full Text PDF

MicroRNAs (miRNAs) are a class of endogenous, non-coding small-molecule RNAs that usually regulate the expression of target genes at the post-transcriptional level. miR156 is one of a class of evolutionarily highly conserved miRNA families. SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor is one of the target genes that is regulated by miR156.

View Article and Find Full Text PDF
Article Synopsis
  • Abiotic and biotic stresses significantly threaten agriculture and food security by affecting plant growth and productivity.
  • Long non-coding RNAs (lncRNAs), which are RNA molecules that don't code for proteins, play a crucial role in regulating gene expression and helping plants respond to these stresses.
  • This research discusses how lncRNAs interact with various plant hormone pathways and highlights their potential for advancing breeding techniques to create stress-resistant crops.
View Article and Find Full Text PDF

Ubiquitination is a specific protein degradation and reversible post-translational modification process that can be reversed by deubiquitinase (DUBs). DUBs can hydrolyze and release ubiquitin in the substrate protein so that the substrate can avoid degradation or change its activity, and it has an impact on plant growth and development, cell cycle, abiotic stress response, and other biological processes. Transcript sequences of potato varieties "DM1-3", "Atlantic" and "Cooperation-88" downloaded from Potato Genome Resources were used for genome-wide identification of the DUB gene family using Hidden Markov Models and verified in the NCBI CD-Search tool.

View Article and Find Full Text PDF

Cotton Verticillium wilt, mainly caused by , has a serious impact on the yield and quality of cotton fiber. Many microRNAs (miRNAs) have been identified to participate in plant resistance to . infection, but the exploration of miRNA's function mechanism in plant defense is needed.

View Article and Find Full Text PDF

Biotic and abiotic stresses are the main constrain of potato ( L.) production all over the world. To overcome these hurdles, many techniques and mechanisms have been used for increasing food demand for increasing population.

View Article and Find Full Text PDF

The autotetraploid potato ( L.) is an important crop in China, and it is widely cultivated from Northeast China to South China. Thousands of varieties are bred by breeding institutions or companies, and distinguishing the different varieties based on morphological characteristics is difficult.

View Article and Find Full Text PDF

Soil salinity has become an increasingly serious problem worldwide, greatly limiting crop development and yield, and posing a major challenge to plant breeding. Basic leucine zipper (bZIP) transcription factors are the most widely distributed and conserved transcription factors and are the main regulators controlling various plant response processes against external stimuli. The bZIP protein contains two domains: a highly conserved, DNA-binding alkaline region, and a diverse leucine zipper, which is one of the largest transcription factor families in plants.

View Article and Find Full Text PDF

Crop plants are vulnerable to various biotic and abiotic stresses, whereas plants tend to retain their physiological mechanisms by evolving cellular regulation. To mitigate the adverse effects of abiotic stresses, many defense mechanisms are induced in plants. One of these mechanisms is the mitogen-activated protein kinase (MAPK) cascade, a signaling pathway used in the transduction of extracellular stimuli into intercellular responses.

View Article and Find Full Text PDF

Sensor-responder complexes comprising calcineurin B-like (CBL) proteins and CBL-interacting protein kinases (CIPKs) are plant-specific Ca receptors, and the CBL-CIPK module is widely involved in plant growth and development and a large number of abiotic stress response signaling pathways. In this study, the potato cv. "Atlantic" was subjected to a water deficiency treatment and the expression of gene was detected by qRT-PCR.

View Article and Find Full Text PDF

One of the main impacts of drought stress on plants is an excessive buildup of reactive oxygen species (ROS). A large number of ·OH, highly toxic to cells, will be produced if too much ROS is not quickly cleared. At the heart of antioxidant enzymes is superoxide dismutase (SOD), which is the first antioxidant enzyme to function in the active oxygen scavenging system.

View Article and Find Full Text PDF

Ubiquitin-conjugating enzymes (E2s/UBC) are components of the ubiquitin proteasome system (UPS), and the ubiquitin-conjugating enzyme variant (UEV) is one of E2s (ubiquitin-conjugating enzymes, UBC) subfamily. The UEVs and UBC13 play an auxiliary role in mediating Lys63-linked polyUb chain assembly, which is correlated with target protein non-proteolytic functions, such as DNA repair or response to stress. However, the collaborative mechanism of (homologue of AtUBC13) and (the UEVs in potato) involved in potato are not fully understood understood.

View Article and Find Full Text PDF

Stomata are specialized portals in plant leaves to modulate water loss from plants to the atmosphere by control of the transpiration, thereby determining the water-use efficiency and drought resistance of plants. Despite that the stomata developmental progression is well-understood at the molecular level, the experimental evidence that miRNA regulates stomata development is still lacking, and the underlying mechanism remains elusive. This study demonstrates the involvement of stu-miR827 in regulating the drought tolerance of potato due to its control over the leaf stomatal density.

View Article and Find Full Text PDF