Interplant communication increases aphid resistance and alters rhizospheric microbes in neighboring plants of aphid-infested cucumbers.

Background: Aphis gossypii Glover is a prevalent phytophagous insect that inflicts significant damage on cucumber plants. Recent studies have provided insights into plant communication and signal transduction within conspecifics. However, understanding of the effect of these communication mechanisms on adjacent cucumbers and their resident aphids, especially in the context of an aphid infestation, is still in its early stages.

Powdery mildew-induced changes in phyllosphere microbial community dynamics of cucumber.

As an important habitat for microorganisms, the phyllosphere has a great impact on plant growth and health, and changes in phyllosphere microorganisms are closely related to the occurrence of leaf diseases. However, there remains a limited understanding regarding alterations to the microbial community in the phyllosphere resulting from pathogen infections. Here, we analyzed and compared the differences in phyllosphere microorganisms of powdery mildew cucumber from three disease severity levels (0% < L1 < 30%, 30% ≤ L2 < 50%, L3 ≥ 50%, the number represents the lesion coverage rate of powdery mildew on leaves).

Cucumis sativus PHLOEM PROTEIN 2-A1 like gene positively regulates salt stress tolerance in cucumber seedlings.

PHLOEM PROTEIN 2-A1 like (PP2-A1) gene is a member of the PP2 multigene family, and the protein encoded by which has the function of stress defense. Based on our previous proteomic study of cucumber phloem sap, CsPP2-A1 protein expression was significantly enriched under salt stress. In this paper, we obtained CsPP2-A1 interfering (CsPP2-A1-RNAi) cucumber by Agrobacterium tumefaciens-mediated method.

The phloem lectin PP2-A1 enhances aphid resistance by affecting aphid behavior and maintaining ROS homeostasis in cucumber plants.

Aphid (Aphis gossypii Glover) attack frequently results in a significant loss of output and deterioration of fruit quality in cucumber (Cucumis sativus L.). Phloem protein 2 (PP2) is conserved as a phloem lectin in plants, and few studies have been conducted on the regulatory mechanism of PP2.

Exogenously applied melatonin alleviates the damage in cucumber plants caused by Aphis goosypii through altering the insect behavior and inducing host plant resistance.

Background: Aphis gossypii Glover is the main pest found in most cucumber-producing areas. Melatonin (MT) has been widely studied in protecting plants from environmental stresses and pathogens. However, little knowledge is available on the impact of MT on insect resistance.

Exogenous melatonin improves the resistance to cucumber bacterial angular leaf spot caused by Pseudomonas syringae pv. Lachrymans.

Pseudomonas syringae pv. Lachrymans (Psl) is a bacterial pathogen that causes cucumber bacterial angular leaf spot (BALS). It is known that melatonin (MT), as a pleiotropic signal molecule, can improve plant stress tolerance, but less information is available about the function of MT on plant resistance to bacteria disease.

Prokaryotic expression, purification, physicochemical properties and antifungal activity analysis of phloem protein PP2-A1 from cucumber.

Phloem protein 2 (PP2) is a protein having lectin properties that can be isolated from the phloem sap. Based on our previous proteomic study of phloem sap of Cucumis sativus, it was found that the expression of PP2 A1-like was significantly up-regulated under salt stress, which may be a molecular mechanism of plant adaptation to stress. This paper carried out the expression and purification of the CsPP2-A1 gene in E.

Understanding of the postgerminative development response to salinity and drought stresses in cucumber seeds by integrated proteomics and transcriptomics analysis.

The postgerminative development is a complex, genetically programmed process, and also the most dangerous period before the developing seedlings reach the autotrophy state. To obtain a comprehensive understanding of postgerminative development mechanism, the study focuses on an integrative analysis on transcriptome, proteome, and microRNA in cucumber seeds under drought and salt stress. Drought and salt stress caused differential expression of 4197 mRNAs, 36 microRNAs and 768 proteins compared with the control, and 827 mRNAs, 364 proteins, and 12 microRNAs were shared by the two stresses.

[Advances in Agrobacterium tumefaciens-mediated transgenic cucumber].

Cucumber (Cucumis sativus) is an important vegetable crop in the world. Agrobacterium-mediated transgenic technology is an important way to study plant gene functions and improve varieties. In order to further accelerate the transgenic research and breeding process of cucumber, we described the progress and problems of Agrobacterium tumefaciens-mediated transgenic cucumber, from the influencing factors of cucumber regeneration ability, genetic transformation conditions and various additives in the process.

Response of proteome and morphological structure to short-term drought and subsequent recovery in Cucumis sativus leaves.

Drought is the primary limitation to plant growth and yield in agricultural systems. Cucumber (Cucumis sativus) is one of the most important vegetables worldwide and has little tolerance for water deficit. To understand the drought stress response strategy of this plant, the responses of cucumber to short-term drought and rewatering were determined in this study by morphological structure and proteomic analyses.

Phloem sap proteome studied by iTRAQ provides integrated insight into salinity response mechanisms in cucumber plants.

Cucumber is an economically important crop as well as a model system for plant vascular biology. Salinity is one of the major environmental factors limiting plant growth. Here, we used an iTRAQ-based quantitative proteomics approach for comparative analysis of protein abundances in cucumber phloem sap in response to salt.

Effect of short-term water deficit stress on antioxidative systems in cucumber seedling roots.

Background: Cucumber is one of the most popular vegetables, and have little tolerance for water stress. The antioxidant defense system is one of major drought defense and adaptive mechanisms in plants, however, relatively few data are available regarding antioxidant systems in responses of cucumber to water deficit. The effect of short-term drought stress on the antioxidant system, lipid peroxidation and water content in cucumber seedlings roots was investigated.

Proteomic analysis of cucumber seedling roots subjected to salt stress.

To understand metabolic modifications in plants under salt stress, the physiological and biochemical responses of cucumber (Cucumis sativus L. cv. Jinchun No.