Preservation of Blood Vessels with an Oxygen Generating Composite.

Damage caused by oxygen deficiency (hypoxia) is one of the major factors limiting tissue and organ preservation time. Cooling tissues slows down metabolic rate of cells thereby prolonging tissue and organ survival sufficiently to allow transport and transplantation within a few hours. Although metabolism is slowed, cells and some enzymes continue to consume oxygen that can render cold stored tissues hypoxic.

Mimicking oxygen delivery and waste removal functions of blood.

In addition to immunological and wound healing cell and platelet delivery, ion stasis and nutrient supply, blood delivers oxygen to cells and tissues and removes metabolic wastes. For decades researchers have been trying to develop approaches that mimic these two immediately vital functions of blood. Oxygen is crucial for the long-term survival of tissues and cells in vertebrates.

Cellular internalization of rod-like nano hydroxyapatite particles and their size and dose-dependent effects on pre-osteoblasts.

Nano hydroxyapatite particles (n-HA) have been reported to promote osteogenic activities of bone-related cells, while inhibiting tumor cell growth, and the biological effects of n-HA are related with the particle size, dose, culture time and cell type. In this work, we prepared n-HA with a strictly controlled rod-like shape and adjustable sizes without any surface chemical contaminations. Using the prepared n-HA, we investigated the size and dose effect of the nano particles on pre-osteoblasts for up to 7 days.