Elucidating loss-of-function mechanisms of monoallelic EPAS1 mutations underlying congenital hypoplastic anaemia in a paediatric anaemia cohort.

HIF-2α, encoded by EPAS1, plays a dominant role in regulating erythropoietin (EPO) production, maintaining the dynamic balance of erythropoiesis. Gain-of-function mutations in EPAS1 cause erythrocytosis. However, anaemia caused by EPAS1 loss-of-function mutations has been confined to only one case report, and the underlying mechanism remains unclear.

Forecasting the distribution range of Hylurgus ligniperda (Fabricius) (Coleoptera: Curculionidae) in the present and future under the influence of climate change.

Hylurgus ligniperda (Fabricius) is an important pest that attacks Pinus species in China. It impacts the vitality of local pine vegetation, reduces the ability to prevent windbreak and sand fixation, and causes ecological loss. MaxEnt and ArcGIS are used to predict and analyze the changes in suitable distribution areas of H.

The chromosome-level genome assembly and annotation of an invasive forest pest Obolodiplosis robiniae.

Biological invasion is a major global problem, leading to the loss of biodiversity and species extinction, and causing huge economic losses to countries. Obolodiplosis robiniae is a major invasive forest pest that has caused economic losses in Asia and Europe. Here, the chromosome- level genome of O.

Fructose-2,6-bisphosphate restores DNA repair activity of PNKP and ameliorates neurodegenerative symptoms in Huntington's disease.

Huntington's disease (HD) and spinocerebellar ataxia type 3 (SCA3) are the two most prevalent polyglutamine (polyQ) neurodegenerative diseases, caused by CAG (encoding glutamine) repeat expansion in the coding region of the huntingtin (HTT) and ataxin-3 (ATXN3) proteins, respectively. We have earlier reported that the activity, but not the protein level, of an essential DNA repair enzyme, polynucleotide kinase 3'-phosphatase (PNKP), is severely abrogated in both HD and SCA3 resulting in accumulation of double-strand breaks in patients' brain genome. While investigating the mechanistic basis for the loss of PNKP activity and accumulation of DNA double-strand breaks leading to neuronal death, we observed that PNKP interacts with the nuclear isoform of 6-phosphofructo-2-kinase fructose-2,6-bisphosphatase 3 (PFKFB3).

RBM25 is required to restrain inflammation via ACLY RNA splicing-dependent metabolism rewiring.

Spliceosome dysfunction and aberrant RNA splicing underline unresolved inflammation and immunopathogenesis. Here, we revealed the misregulation of mRNA splicing via the spliceosome in the pathogenesis of rheumatoid arthritis (RA). Among them, decreased expression of RNA binding motif protein 25 (RBM25) was identified as a major pathogenic factor in RA patients and experimental arthritis mice through increased proinflammatory mediator production and increased hyperinflammation in macrophages.

Effectiveness and safety of Chinese herbal footbaths as an adjuvant therapy for dysmenorrhea: a systematic review and meta-analysis.

Objectives: To evaluate the effectiveness and safety of Chinese herbal footbaths (CHF) as an adjunctive therapy in managing dysmenorrhea.

Methods: Ten electronic databases were searched to identify eligible randomized clinical trials (RCTs) from inception until June 2023. Outcome measurements encompassed the total effective rate, visual analog scale (VAS) score of pain intensity, Cox menstrual symptom scale (CMSS) score, symptom score, Traditional Chinese Medicine (TCM) syndrome scale, and any reported adverse events.

Development of an investigation form for hemodialysis infection outbreak: Identifying sources in the early stage.

Background: There are many infectious factors causing the outbreak of hemodialysis infection, which may easily lead to the delay of investigation and treatment. This study aimed to develop an investigation form for hemodialysis infection outbreak (HIO), and to identify sources of outbreak in early stage.

Methods: After an exhaustive literature review, we used the Delphi method to determine the indicators and relative risk scores of the assessment tools through 2 rounds of specialist consultation and overall consideration of the opinions and suggestions of 18 specialists.

Construction of a postoperative infection outbreak investigation form: A tool for early detection and control measures.

Background: To develop an investigation form for postoperative infection outbreak (PIO), and to identify sources of the outbreak in the early stage.

Methods: After an exhaustive literature review, we used the Delphi method to determine the indicators and relative risk scores of the assessment tools through 2 rounds of specialist consultation and overall consideration of the opinions and suggestions of 20 specialists.

Results: A total of 203 studies of PIO were eligible for inclusion.

Fructose-2,6-bisphosphate restores DNA repair activity of PNKP and ameliorates neurodegenerative symptoms in Huntington's disease.

Huntington's disease (HD) and spinocerebellar ataxia type 3 (SCA3) are the two most prevalent polyglutamine (polyQ) neurodegenerative diseases, caused by CAG (encoding glutamine) repeat expansion in the coding region of the huntingtin (HTT) and ataxin-3 (ATXN3) proteins, respectively. We have earlier reported that the activity, but not the protein level, of an essential DNA repair enzyme, polynucleotide kinase 3'-phosphatase (PNKP), is severely abrogated in both HD and SCA3 resulting in accumulation of double-strand breaks in patients' brain genome. While investigating the mechanistic basis for the loss of PNKP activity and accumulation of DNA double-strand breaks leading to neuronal death, we observed that PNKP interacts with the nuclear isoform of 6-phosphofructo-2-kinase fructose-2,6-bisphosphatase 3 (PFKFB3).

Exploring the mechanism of Cassiae semen in regulating lipid metabolism through network pharmacology and experimental validation.

Background: Multiple studies have assessed the role of Cassiae semen (CS) in regulating lipid metabolism. However, the mechanism of action of CS on non-alcoholic fatty liver disease (NAFLD) has seen rare scrutiny.

Objective: The objective of this study was to explore the regulatory mechanism of CS on lipid metabolism in NAFLD.

Flipped classroom improves student learning outcome in Chinese pharmacy education: A systematic review and meta-analysis.

The application of flipped classroom (FC) pedagogy has recently become increasingly popular in Chinese pharmacy education. However, its effectiveness in improving student learning has not yet been assessed. This study aimed to evaluate the effects of teaching with such pedagogical approach by examining studies that compare the FC approach with the traditional lecture-based learning (LBL) module through a systematic review and meta-analysis.

Circulating plasma galectin-3 predicts new-onset atrial fibrillation in patients after acute myocardial infarction during hospitalization.

Background: New-onset atrial fibrillation (NOAF) is a common complication in patients with acute myocardial infarction (AMI) during hospitalization. Galectin-3 (Gal-3) is a novel inflammation marker that is significantly associated with AF. The association between post-AMI NOAF and Gal-3 during hospitalization is yet unclear.

Numerical simulation of vegetation evolution in compound channels.

The study of vegetation evolution is essential for further understanding of biogeographic feedback and ecological restoration. In this paper, a vegetation evolution model based on velocity threshold (scaled by the average flow velocity of the bare bed) was established to simulate the vegetation evolution process in compound channels. In this model, the effect of vegetation on water flow was generalized as equivalent Manning coefficient, and the velocity field was obtained by solving two-dimensional shallow water equations.

Analysis of contaminant dispersion in open channel with two streambank-absorption boundaries.

Boundary absorption intensity can affect the contaminant depletion capacity within rivers, and the process of spatial contaminant cloud expansion is complicated with the consideration of irreversible absorption boundaries at riverbanks. Nonuniformity of concentration distribution appears in spatial concentration distribution, especially in the transverse direction, which is caused by the absorption capacity difference between two riverbanks. A model for illustrating the performance of environmental dispersion with two irreversible absorption banks in 2D space is given in this work.

A random displacement model of sediment transport in ice-covered alluvial channel flows.

There is a need for developing efficient models to simulate the sediment transport phenomenon in ice-covered alluvial channel flows, which is essential in enriching the theory of riverbed evolution. This study establishes a random displacement model parameterized with the time-averaged streamwise velocity U(z), the sediment settling velocity ω(z), and the turbulent diffusion coefficient D(z) to calculate the suspended sediment concentration and the longitudinal dispersion coefficient for ice-covered alluvial channels. The proposed model is first validated to determine if it could be used to predict the sediment concentration profiles by comparing to limited experiments published in the literature.

Responses of cyprinid (Ancherythroculter nigrocauda) to flow with a semi-circular cylinder patch.

Flows in river habitats are characterized by unsteady turbulence due to the existence of woody debris, boulders and vegetation. As a representative aquatic species, fish is important for the riverine ecosystems, with its complex behavioural responses to turbulent flows. Previous studies investigated the fish-vortices interaction with vortex streets by placing objects with simplified geometries centred at the flow.

[Time comparison of artificial airway establishment in operating room and on slope using endotracheal intubation and laryngeal mask].

Objective: To compare the completion time of endotracheal intubation and laryngeal mask implantation in operating room and on slope of ski resort, and to discuss the optimal method of estab-lishing artificial airway on slope of ski resort.

Methods: The simulator was placed with the head under the feet on slope of ski resort. The artificial airway was established by tracheal intubation assisted by video laryngoscope (endotracheal intubation group) and laryngeal mask placement (laryngeal mask group) respectively by an anesthesiologist who wore full set of ski suits, helmets, goggles, gloves and ski boots.

First Report of Root Rot Caused by Phytophthora acerina on Metasequoia glyptostroboides in China.

Metasequoia glyptostroboides Hu & W. C. Cheng (Taxodiaceae), commonly called the Chinese redwood or dawn redwood, is a well-known "living fossil" and rare relict plant species endemic to China, which has been successfully cultivated throughout the world (Ma 2007).

Action Mechanism Underlying Improvement Effect of Fuzi Lizhong Decoction on Nonalcoholic Fatty Liver Disease: A Study Based on Network Pharmacology and Molecular Docking.

Objective: This study aimed to decipher the bioactive compounds and potential mechanism of traditional Chinese medicine (TCM) formula Fuzi Lizhong Decoction (FLD) for nonalcoholic fatty liver disease (NAFLD) treatment via an integrative network pharmacology approach.

Methods: The candidate compounds of FLD and its relative targets were obtained from the TCMSP and PharmMapper web server, and the intersection genes for NAFLD were discerned using OMIM, GeneCards, and DisGeNET. Then, the PPI and component-target-pathway networks were constructed.

Flow characteristics in partially vegetated channel with homogeneous and heterogeneous layouts.

This study presents the experimental results of the flow characteristics, such as the flow adjustment, velocity profiles, mixing layer, and the momentum exchange, in the partially vegetated channel with homogeneous and heterogeneous layouts. Three cases are considered, including two homogeneous canopies with uniform sparse and dense vegetation patches respectively, and a heterogeneous canopy consisting of alternating patches of both densities. Results show that heterogeneous canopy requires a longer adjustment distance to reach the quasi-equilibrium region, compared with the homogenous canopy of the same density.

An Exploration and Technical Notes for Advanced Airway Management on the Ski Slope: A Simulation Experiment.

Background: Skiing is a high-risk winter sport, and the rate of injury fatality is the highest compared to other winter sports. During skiing rescue, the harsh natural environments will increase the difficulty of artificial airway establishment. There has been no research focusing on the establishment of the artificial airway during skiing rescue site.

A new model for settling velocity of non-spherical particles.

The settlement of non-spherical particles, such as propagules of plants and natural sediments, is commonly observed in riverine ecosystems. The settling process is influenced by both particle properties (size, density, and shape) and fluid properties (density and viscosity). Therefore, the drag law of non-spherical particles is a function of both particle Reynolds number and particle shape.

Transverse distribution of the streamwise velocity for the open-channel flow with floating vegetated islands.

Floating vegetation islands (FVIs) have been widely utilized in various river ecological restoration projects due to their ability to purify pollutants. FVIs float at the surface of shallow pools with their roots unanchored in the sediment. Biofilm formed by roots under islands filters nutrients and particles in the water flowing through it.

Sumoylation of Human Parainfluenza Virus Type 3 Phosphoprotein Correlates with A Reduction in Viral Replication.

Human parainfluenza virus type 3 (HPIV3), a member of the Paramyxoviridae family, can cause lower respiratory disease in infants and young children. The phosphoprotein (P) of HPIV3 is an essential cofactor of the viral RNA-dependent RNA polymerase large protein (L). P connects nucleocapsid protein (N) with L to initiate genome transcription and replication.