Discovery and biosynthesis of macrophasetins from the plant pathogen fungus .

3-Decalinoyltetramic acids (DTAs) are a class of natural products with chemical diversity and potent bioactivities. In fungal species there is a general biosynthetic route to synthesize this type of compounds, which usually features a polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) and a lipocalin-like Diels-Alderase (LLDAse). Using a synthetic biology approach, combining the bioinformatics analysis prediction and heterologous expression, we mined a PKS-NRPS and LLDAse encoding gene cluster from the plant pathogenic fungus and characterized the cluster to be responsible for the biosynthesis of novel DTAs, macrophasetins.

Genome mining and biosynthesis of the Acyl-CoA:cholesterol acyltransferase inhibitor beauveriolide I and III in Cordyceps militaris.

Ascomycete fungi Cordyceps are widely used in traditional Chinese medicine, and numerous investigations have been carried out to uncover their biological activities. However, primary researches on the physiological effects of Cordyceps were committed using crude extracts. At present, there are only a few compounds which were comprehensively characterized from Cordyceps, partial owing to the low production.

Isolation and characterization of a new cytotoxic polyketide-amino acid hybrid from ATCC 42464.

Fungi are a rich source of novel anticancer compounds. Bioassay-guided isolation has led to the isolation of four polyketide-amino acid hybrid compounds with -fused decalin system from the fungus ATCC 42464 (= ATCC 42464): myceliothermophins A, B, E and F (-). The structure of the new compound (myceliothermophin F, compound ) was clearly determined by a combination of nuclear magnetic resonance (NMR) analysis and high-resolution electrospray ionisation mass spectroscopy (HRESIMS).

Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides.

Partially acetylated chitosan oligosaccharides (COS), which consists of N-acetylglucosamine (GlcNAc) and glucosamine (GlcN) residues, is a structurally complex biopolymer with a variety of biological activities. Therefore, it is challenging to elucidate acetylation patterns and the molecular structure-function relationship of COS. Herein, the detailed deacetylation pattern of chitin deacetylase from , CDA₂, was studied.

[In vitro cultivation of dendritic cells with serum-free medium].

This study was aimed to investigate the protocol in vitro to incubate the dendritic cell (DC) derived from peripheral blood monocytes using serum-free medium X-VIVO 20. Peripheral blood monocytes from healthy donors were treated with 100 ng/ml GM-CSF and 500 U/ml IL-4, respectively. After cultivation for 6 days, they were treated with 100 ng/ml calcium ionophore A23187.