[Effect of Toxoplasma gondii prugniaud strain infection in pregnant mice on the learning ability of the F1 generation].

Objective: To study the effect of Toxoplasma gondii prugniaud strain infection on female reproductive toxicity in mice and learning ability of their F1 generation.

Methods: Thirteen ICR mice were each infected intragastrically with 10 T. gondii cysts on the 15th day of pregnancy (late stage pregnancy).

[Dynamic pathologic changes in pigs infected with Taenia solium].

Four three-way crossed hybrid pigs were infected each with 80,000 Taenia solium eggs. One pig was sacrificed respectively at 40, 80, 120, and 150 d post-infection, and tissue samples containing cysticerci were collected for pathological sections. The results showed that in 40-80 days, cysticerci in the liver were surrounded by membrane formed with the hyperplastic fibrous connective tissue and inflammatory cells.

[Total protein analysis by two-dimensional electrophoresis in cysticerci of Taenia solium and Taenia asiatica].

Two 20-day-old three-way crossed hybrid pigs were infected with 80000 Taenia solium or T. asiatica eggs, respectively. Immature cysticerci of the two species in liver were collected at 40 days after infection.

[Histopathology changes in mice infected with Toxoplasma gondii Prugniaud strain].

Objective: To observe the symptoms and dynamic changes of histopathology in the organs from ICR mice infected by Toxoplasma gondii Prugniaud strain.

Methods: Thirty ICR mice were infected intraperitoneally with cysts, 10 cysts per mouse. 16 mice were injected with PBS.

[Research progress on molecular identification and biologic behavior of Taenia saginata in Western China].

This article reviews the epidemiological investigation of taeniasis saginata in 10 counties of 7 provinces/ autonomous regions in western China. The morphological observation of adult worms, molecular identification of 10 isolates of the worms, experimental infection on pigs and cattle with Taenia saginata and T. asiatica, observation on development and biology behavior of cysticercus, and pathological changes in the intermediate host pig and cattle revealed that T.

[Informatics analysis of malate dehydrogenase from Taenia saginata asiatica].

Tools from bioinformatics websites such as NCBI, ExPaSy were used for the analysis. The malate dehydrogenase full-length gene from Taenia saginata asiatica was 1 212 bp in length, with a coding region of 30-1 028 bp and coding 332 amino acids. It was a complete and full-length gene compared with the homologues in GenBank.

[Analysis of Toxoplasma gondii specific antigens by two dimensional electrophoresis and western blotting].

The total protein of Toxoplasma gondii RH strain tachyzoites was separated by two-dimensional electrophoresis (2-DE), and Western blotting was performed to find out distinct antigens. 209 spots were detected through Coomassie brilliant blue-stained small gels (7x8 cm, pH 3-10). Western blotting showed 17 specific antigen spots with sera of the experimental group and two nonspecific spots with the control sera.

[Cloning and prokaryotic expression of malate dehydrogenase gene of Taenia saginata asiatica and immunogenicity analysis of the recombinant protein].

Objective: To clone and express the lactate dehydrogenase (LDH) gene of Taenia saginata asiatica and analyze the immunogenicity of the recombinant protein.

Methods: By screening the full length cDNA plasmid library, the coding region of LDH was amplified with PCR, and cloned into the prokaryotic expression vector pET-30a (+), then expressed in E. coli BL21 with IPTG induction.

[Morphological observation on the adult worms of Taenia saginata in western China].

Objective: To investigate the morphological characteristics of the adult worms of Taenia saginata from four areas of Western China.

Methods: 42, 41, 7 and 18 integral worms of Taenia saginata were collected from Duyun and Congjiang of Guizhou Province, Wushi of Xinjiang, and Lhasa of Tibet respectively. The length of worms was measured and the segments were counted.

Observations on pathological and histochemical changes in piglet livers infected with Taenia saginata asiatica.

To study the pathological and histochemical characteristics of lesions in piglet livers infected with Taenia saginata asiatica (T. saginata asiatica) throughout the different stages, piglets were fed with eggs of T. saginata asiatica and raised in isolation in an animal center to establish the T.

[The random amplified polymorphic DNA identification of 9 Taenia saginata isolates from four provinces].

Objective: To make molecular identification for 9 isolates of Taenia saginata from 4 provinces.

Methods: Genomic DNA was extracted from the segments of adult tapeworms collected from Taoyuan of Taiwan (TW1), Duyun of Guizhou (DY1, DY2), Congjiang of Guizhou (CJ1, CJ2, CJ3, CJ4), Dali of Yunnan (DL1) and Wushi of Xinjiang (XJ1) respectively. PCRs were carried out with 13 random primers.

[Effect of gamma-interferon on hepatic granuloma formation in mice infected by cysticerci of Taenia saginata asiatica].

Immunohistochemical streptavidin biotin-peroxidase complex method was used to investigate the effect of gamma-interferon (IFN-gamma) on the hepatic granuloma formation and liver fibrosis in mice infected with Taenia saginata in Duyun area of Guizhou Province. The results reveal contrary relation between the level of IFN-gamma in the liver and the degree of liver fibrosis (p<0.01).

[Change of lipid, esterase and lipase in mosquito larvae infected with Lagenidium giganteum].

Objective: To explore possible physiological and biochemical mechanisms of Lagenidium giganteum infection in killing mosquito larvae.

Methods: The content of lipid and the activities of esterase and lipase between the normal and infected mosquito larvae were observed with histochemical method. The results were photomicrograph and analysed by image analysis using computer.

[Epidemiological investigation of Taenia saginata asiatica in Duyun, Guizhou and detection of amino acids and elements of adult worms].

Objective: To study epidemiological factors of taeniasis and to detect amino acid and element components of adult worms in Duyun of Guizhou Province.

Methods: 1. Traditional methods were used for epidemiological investigation.

[Identification of Taenia saginata by mtCO I in four areas of Yunnan and Guizhou provinces].

Objective: To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province.

Methods: Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunit I (mtCO I) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package.

Results: The mtCO I gene sequences of Lanping sample were identical to that of T.

[Changes of elements in Culex quinque fasciatus larvae after infected by Lagenidium giganteum (Oomycetes: Lagenidiales)].

Objective: To analyze the quantitative change of 18 elements in Culex quinque fasciatus Larvae infected by Lagenidium giganteum (Oomycetes: lagenidiales), and to explore possible mechanism of mosquito-killing by the fungus.

Methods: Elements in the mosquito larvae at different developmental stages from infected and non-infected (control) groups were detected with automatic bioassay machine.

Results: At the early stage of infection, content of Ca and Cu in the infected group was markedly lower while the content of Co and Cd were higher than the control.