Publications by authors named "Huahao Shao"
Article Synopsis
- The study focuses on a new nanopore sensor that uses a DNA walker and an autocatalytic reaction to detect DNA methyltransferases (MTases), important for understanding gene regulation and cancer.
- The sensor operates by methylating and cleaving a hairpin DNA structure in the presence of Dam MTase, triggering a series of reactions that amplify the signal and produce numerous DNA nanowires.
- This enhanced detection mechanism can identify extremely low levels of Dam MTase and can be adapted for M.SssI MTase, showcasing its potential in advanced biosensing applications.
In this study, a polyethyleneimine (PEI)/Zr-functionalized nanofluidic sensing platform based on nonlinear hybridization chain reaction (NHCR) was developed for PNK activity assay. With the existence of PNK, the hairpin H was cleaved by λ exonuclease, liberating the initiator T-DNA. Then T-DNA triggered the nonlinear HCR in solution and the reaction products were absorbed onto the nanopore, which changed the surface charge of nanofluidic device and could be detected by current-voltage characteristic curves.
View Article and Find Full Text PDFThe accurate and ultrasensitive detection of multiple methyltransferases was in great request for clinical diagnosis and epigenetic therapy. Here, a novel fluorescence assay was proposed for ultrasensitive CpG methyltransferase (M.SssI) and DNA adenine methyltransferase (Dam) activity detection based on hyperbranched rolling circle amplification (HRCA) and DNA walkers.
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