[The effects of p38MAPK and HBxAg on cell proliferation and apoptosis in human hepatocarcinogenesis].

Objective: To investigate the effects of host-derived p38 mitogen-activated protein kinase subunit 38 (p38MAPK) and the hepatitis B virus X antigen (HbxAg) on cell proliferation and apoptosis in human hepatocellular carcinoma (HCC), and to study the mechanism underlying hepatocarcinogenesis.

Methods: Liver tissues were biopsied from healthy individuals and patients with chronic hepatitis B (CHB), liver cirrhosis, paratumor cirrhosis, and HCC. Immunohistochemical staining was used to detect expressions of HBxAg, p38MAPK, cell cycle G2/M phase-related factors (cdc25B, p34cdc2, cyclin B1), and cell proliferation factor ki-67.

[Role of p38MAPK in apoptosis of human paratumor cirrhosis hepatocellular cell line QSG-7701 induced by cisplatin].

Objective: To investigate the roles of p38 MAPK in apoptosis of the normal liver cell, the paratumor cirrhosis hepatocellular cell and the hepatocellular carcinoma cell.

Methods: Three cell lines were adopted (the normal liver cell line HL-7702, the paratumor cirrhosis hepatocellular cell line QSG-7701 and the hepatocellular carcinoma cell line QGY-7703) and treated with Diamminedichloroplatin (DDP, cisplatin) and p38MAPK inhibitor SB203580. The apoptosis and cell cycles were detected by flow cytometry and electromicroscopy.

Effect of qi-tonifying and stasis-eliminating therapy on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats.

Objective: To investigate the effects and mechanism of qi-tonifying and stasis-eliminating (QTSE) therapy on the expression of vascular endothelial growth factor (VEGF) and its receptors Flt-1 and Flk-1 in the brains of intracerebral hemorrhagic (model) rats.

Methods: One hundred and eighty Sprague-Dawley rats were randomly divided into six groups: the normal group (n=5), the sham-operative (SO) group (n=35), the model group (n=35), the QTSE group (n=35), the QT group (n=35) and the SE group (n=35). All the rats except those in the normal group and SO group were established into an intracerebral hemorrhage(ICH) model by intracerebral injection of collagenase type VII and the latter three were orally administered with Buyang Huanwu Decoction (a classical recipe for QTSE) or with some of its components for qi-tonification and for stasis-elimination, respectively.

Learning large-scale spatial relationships in a maze and effects of MK-801 on retrieval in the rhesus monkey.

Monkeys have strong abilities to remember the visual properties of potential food sources for survival in the nature. The present study demonstrated the first observations of rhesus monkeys learning to solve complex spatial mazes in which routes were guided by visual cues. Three monkeys were trained in a maze (6 m x 6 m) included of four different mazes.

[Effects of naoyian granule on the expression of IL-6 mRNA and protein in the experimental intracerebral hemorrhagic brain in rats].

Objective: To explore the protective mechanisms of the traditional Chinese medicine complex Naoyian granule (NYA) on intracerebral hemorrhagic (ICH).

Methods: ICH rat models were established by stereotaxically injecting 0.4 U type VII collagenase into the right globus pallidus (GP), the expression of IL-6mRNA and protein were investigated by in situ hybridization and Western blotting.

[Effect of naoyian on phosphorylated Akt in the neural stem cell injured with anoxia].

Objective: To determine the effect of naoyian (NYA) on the expression of phosph-Akt in neural stem cells injured with anoxia.

Methods: The hippocampal neural stem cells (NSC) taken from the Sprageu-Dawley rat brain at 5 days after birth were cultured in DMEM/F12 solution containing EGF and bFGF. After being treated with anoxia for 6 hours, the neural stem cells were incubated in solution containing the NYA serum or normal serum.

Expression and hypermethylation of p27 kip1 in hepatocarcinogenesis.

Aim: To study the expressions of p27 kip1 protein and p27mRNA, the hypermethylation of p27 kip1 and the relation between them in various stages of hepatocarcinogenesis.

Methods: p27 protein and p27mRNA were detected by immunohistochemical staining and in situ hybridization respectively in 68 cases of normal liver, liver cirrhosis, pericancerous cirrhosis and hepatocellular carcinoma (HCC). The hypermethylation of p27 kip1 was detected by methylation-specific PCR (MSP) in 44 cases of normal liver, liver cirrhosis, and HCC.

[Effects of naoyian serum on VEGF protein expression in cultured rat cerebral microvascular endothelial cell with hypoxia].

Objective: To determine the effects of naoyian (NYA) serum on the expression of vascular endothelial growth factor (VEGF) protein in cultured rat cerebral microvascular endothelial cell (RCMEC) with hypoxia.

Methods: NYA serum was separated from rat heart which had been filled stomach with NYA successively for 3 days. The rat cerebral microvascular endothelial cells were taken from the Sprageu-Dawley rat brain at postborn 7 days.

Activation of endogenous neural stem cells in experimental intracerebral hemorrhagic rat brains.

Background: Many researchers suggest that adult mammalian central nervous system (CNS) is incapable of completing self-repair or regeneration. And there are accumulating lines of evidence which suggest that endogenous neural stem cells (NSCs) are activated in many pathological conditions, including stroke in the past decades, which might partly account for rehabilitation afterwards. In this study, we investigated whether there was endogenous neural stem cell activation in intracerebral hemorrhagic (ICH) rat brains.

[Characteristics of Bcl-2 and Bax expressions in rat brain after experimental intracerebral hemorrhage and intervention of naoyian granule].

Objective: To investigate the characteristics of Bcl-2 and Bax expressions in rat brain after experimental intracerebral hemorrhagic (ICH), and to determine the protective mechanisms of the Traditional Chinese medicine complex, naoyian granule (NYA).

Methods: ICH models were induced according to Rosenbergs method. The rats were divided into the model group, the NYA-treatment group and the sham group.

[Comparison and analysis of expression of c-myc and p16 in cervical carcinoma].

Background & Objective: Activation of proto-oncogene and inactivation of tumor suppressor genes is related to the carcinogenesis of many tumors. It is still unclear whether abnormal expression of c-myc and p16 cooperate in the occurrence and progression of cervical carcinoma, and whether there exists a connection between the expression of two genes and the chemotherapy response of cervical carcinoma. This study was designed to investigate the correlation between the expression of c-myc and p16 and their roles in the genesis and development of the uterine cervical carcinoma and chemotherapy response.

Detection of HBV, PCNA and GST-pi in hepatocellular carcinoma and chronic liver diseases.

Aim: To investigate the change of HBV DNA, PCNA and GST-pi in chronic liver disease and hepatocellular carcinoma (HCC).

Methods: Hepatitis B surface antigen (HBsAg), proliferating cell nuclear antigen (PCNA) and glutathione S-transferases (GST-pi) were detected by immunohistochemical staining and HBV DNA was detected by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded sections with a total of 111 specimens of chronic hepatitis, liver cirrhosis, paratumorous tissue, HCC and normal liver tissue.

Results: The positive rates of HBsAg and HBVDNA were 62.

[Effects of nao-yi-an granule on the intercellular expression of IL-6 in the experimental intracerebral hemorrhagic brain of rats].

Objective: To define the cellular localization of IL-6 and the effects of the traditional Chinese medicine complex nao-yi-an granule (NYA) on the intercellular expression of IL-6 in the experimental intracerebral hemorrhagic (ICH) brain of rats, so as to investigate the efficacy mechanisms of NYA on ICH.

Methods: ICH rat models were established by stereotaxically injecting 0.4 U type VII collagenase into the right globus pallidus (GP).

[Effects of nao-yi-an granule on bcl-2 expression in the rat brain after experimental cerebral hemorrhage].

Objective: To investigate the protective effects and mechanisms of the traditional Chinese medicine complex, nao-yi-an granule (NYA) on experimental intracerebral hemorrhage (ICH) in rats.

Methods: ICH models anesthesized were induced by stereotaxical injection of 2 microliters normal saline containing 0.4 U bacterial collagenase(type VII) into the right globas pallidus(GP), and the protein expression of bcl-2 in the rat brain after ICH was detected with the immunohistochemistry method, with which the effects of NYA were observed.

[Expression of GST-pi and HBV infection in hepatocellular carcinoma].

Background & Objective: The expression of glutathione S-transferases(GST-pi) might abnormally increase in many carcinogenesis, and the alteration of GST-pi preceded than that alteration of cell morphology. This study was designed to investigate the expression of glutathione S-transferases (GST-pi) and its relationship with hepatitis B virus (HBV) infection in hepatocellular carcinoma (HCC).

Methods: Hepatitis B surface antigen (HBsAg), Hepatitis B core antigen (HBcAg), and GST-pi were detected by immunohistochemical staining and HBV DNA was detected by in situ hybridization (ISH) in total 86 samples of chronic hepatitis, liver cirrhosis, paratumor cirrhosis, HCC, and normal liver tissue.