Mechanical study of alisol B 23-acetate on methionine and choline deficient diet-induced nonalcoholic steatohepatitis based on untargeted metabolomics.

Alisol B 23-acetate (AB23A) has been demonstrated to have beneficial effects on nonalcoholic steatohepatitis (NASH). However, the mechanisms of AB23A on NASH remain unclear. This study aimed to investigate the mechanisms underlying the metabolic regulatory effects of AB23A on NASH.

Synergy effects of Methylomonas koyamae and Hyphomicrobium methylovorum under methanethiol stress.

Methanotrophs are able to metabolize volatile organic sulfur compounds (VOSCs), excrete organic carbon during CH oxidation, and influence microbial community structure and function of the ecosystem. In return, microbial community structure and environmental factors can affect the growth metabolism of methanotrophs. In this study, Methylomonas koyamae and Hyphomicrobium methylovorum were used for model organisms, and methanethiol (MT) was chosen for a typical VOSC to investigate the synergy effects under VOSC stress.

The first two cases of transcatheter mitral valve repair with ARTO system in Asia.

Background: MAVERIC (Mitral Valve Repair Clinical Trial) validates the safety and efficacy of the ARTO system. We here report the first two successful cases of utilizing the ARTO system in patients with symptomatic heart failure (HF) with functional mitral regurgitation (FMR) in Asia.

Methods: Two patients, aged 70 and 63, had severe HF with FMR.

Anti-oxidation and anti-aging activity of polysaccharide from Malus micromalus Makino fruit wine.

Malus micromalus Makino fruit wine was used as a raw material and a water soluble polysaccharide (MWP-2) was successfully separated from it by fractionated extraction, alcohol precipitation, macroporous resin purification, cellulose column purification, running water dialysis, and vacuum freeze drying. The structure of MWP-2 was preliminarily analyzed by high performance gel-permeation chromatography (HPGPC), ion chromatograph (IC) and FT-IR. The anti-oxidation and the anti-aging activity of MWP were studied.

[Expression and Regulatory Effect of miR-30b on Dynamin in Cochlear Hair Cells].

Objective: To determine the expression of miR-30b in hair cells of mice,and its regulatory effect on the target gene 1 and expression of Dynamin,the key protein of synaptic endocytosis in inner hair cells.

Methods: The basilar membrane of cochlear in adult C57 mice was obtained. The expression of miR-30b in the hair cells was detected by in situ hybridization.

Conversion of sulfur compounds and microbial community in anaerobic treatment of fish and pork waste.

Volatile sulfur compounds (VSCs) are not only the main source of malodor in anaerobic treatment of organic waste, but also pose a threat to human health. In this study, VSCs production and microbial community was investigated during the anaerobic degradation of fish and pork waste. The results showed that after the operation of 245 days, 94.

[Effect of atopy on serum glucocorticoid receptor levels in children with bronchiolitis].

Objective: To investigate the effect of atopy on the expression of glucocorticoid receptors in children with bronchiolitis.

Methods: ELISA was used to measure the changes in the serum levels of glucocorticoid receptor α (GRα) and glucocorticoid receptor β (GRβ) in the bronchiolitis group (77 children, including 34 children with atopy) and pneumonia group (68 children). Thirty-eight children who were prepared to undergo surgeries for non-infectious diseases and had no atopy or family history of allergic diseases were enrolled as the control group.

Fertility-Preserving Treatments in Patients with Gynecological Cancers: Chinese Experience and Literature Review.

We conducted a retrospectively reviewed of the literature published of patients underwent fertility-preserving treatments for cervical, endometrial and ovarian cancers using the WANFANG database in Chinese. A majority were retrospective studies and case reports. With cervical cancer, radical trachelectomy(RT) in combination with pelvic lymphadenectomy could preserve the fertility of patients with early stage IA1-IB1 cancers, Tumor size≤2 cm should be emphasized as the indication of RT in considering of the higher recurrent rate in patients with tumor size>2 cm.

[Treating chronic persistent bronchial asthma children with abnormal myocardial enzyme spectrum by Yupingfeng powder: an efficacy observation].

Objective: To observe the clinical efficacy of treating chronic persistent bronchial asthma (CPBA) children with abnormal myocardial enzyme spectrum (AMES) by Yupingfeng Powder (YP) combined routine therapy.

Methods: From January 2010 to December 2012, 156 CPBA children patients with AMES were randomly assigned to the treatment group (80 cases) and the control group (76 cases). All patients received routine treatment (inhaled corticosteroids and/or leukotriene regulator).

Factors Associated with Performance-based Physical Function of Older Veterans of the PLAAF: A Pilot Study.

[Purpose] This study investigated the factors associated with performance-based physical function of older veterans of the People's Liberation Army Air Force of China (PLAAF). [Subjects and Methods] A cross-sectional survey of 146 older veterans of the PLAAF was carried out. Their physical function was measured using the Chinese Mini-Physical Performance Testing (CM-PPT).

[Cadmium and selected heavy metals in soils of Jianping area in Wushan County, the Three Gorges region: distribution and source recognition].

Abnormally high cadmium (Cd) concentrations were existed in soils of Jianping area in the Three Gorges region, but little information is available about the geochemical distribution of Cd and source identification. In the present paper, total contents of Cd and other selected heavy metal elements, the characteristics of soil, the contents of Cd in typical soil profiles, and chemical forms of Cd in selected soils were studied. The correlation analysis, the principal component analysis, and the comparison between different soil types were conducted.

[Regulation of claudin-4 gene expression in endometrial adenocarcinoma Ishikawa cell line by progesterone].

Objective: To explore the regulation of claudin-4 expression in endometrial adenocarcinoma cell lines by progesterone.

Methods: Ishikawa cells were treated with various concentrations of megestrol acetate (MA: 2, 5, 10, 15, 20 mg/L). After cultured for 24, 48 and 72 hours, cells growth were measured by methyl thiazolyl tetrazolium (MTT).

[Relationship between serum S100B protein level and brain damage in preterm infants].

Objective: To study changes of serum S100B protein level in preterm infants with brain damage and its role.

Methods: Forty-seven preterm infants were classified into 3 groups based on the results of brain ultrasound and MRI: brain white matter damage (WMD; n=13), brain but not white matter damage (non-WMD; n=14) and control (no brain damage; n=20). Blood samples were collected within 24 hrs, 72 hrs and 7 days after birth.

[Combined use of tandem mass spectrometry with urine gas chromatography/mass spectrometry is useful for diagnosis of inborn errors of metabolism in children].

Objective: Many inborn errors of metabolism have similar presenting clinical manifestations, making early diagnosis difficult. We report our experience with tandem mass spectrometry combined with urine gas chromatography/mass spectrometry as a means of definitively diagnosing inborn errors of metabolism.

Methods: One hundred and thirty-two children with suspected inborn errors of metabolism but without specific clinical manifestations, admitted to the Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine between June 1, 2003 and September 30, 2006, were studied.

[Relationship between pain symptoms and clinico-pathological features of pelvic endometriosis].

Objective: To study the relationship between pain symptoms and the clinico-pathological features of pelvic endometriosis (EM).

Methods: One hundred thirty patients with laparoscopic diagnosis of EM were studied retrospectively and the relationship between pain symptoms including dysmenorrhea, chronic pelvic pain (CPP), dyspareunia and dyschezia and the anatomical features of pelvic endometriosis were evaluated.

Results: One hundred (76.

[Visual and histologic analysis of laparoscopic diagnosis of endometriosis].

Objective: To determine the characteristics of anatomical distribution of pelvic endometriosis and the correlation between visual and histologic findings of endometriosis at laparoscopy.

Methods: A prospective study of 62 patients undergoing laparoscopy for the pelvic pain, infertility and/or pelvic masses was carried out. All lesions with the diagnosis of endometriosis laparoscopically were excised and examined pathologically.

[Correlative factors analysis of recurrence of endometriosis after conservative surgery].

Objective: To determine the correlative factors with recurrence of endometriosis after conservative surgery.

Methods: A cohort study was performed on 285 patients who had a minimum of 36 months of follow-up after conservative surgery for endometriosis. All patients underwent clinical interview, physical examination and ultrasonography.

[Effect of different surgical approaches on quality of life and cost-consequence].

Objective: To evaluate the effects of total abdominal hysterectomy (TAH), laparoscopic hysterectomy (LH) and transvaginal hysterectomy (TVH) on quality of life, cost and health care utilization.

Methods: A study of women who underwent elective hysterectomy was performed to assess the effect of operations on quality of life using WHO quality of life-bref before operation, at the 4th, 14th and 28th day after operation, respectively. The data of total cost and health care utilization were also analyzed.

[Clinical characteristics of abdominal wall endometrioma and its recurrence-related factors].

Objective: To analyze the clinical characteristics, and treatment, recurrence-related factors of abdominal wall endometriomas (AWE).

Methods: Fifty-seven cases of AWE treated at Peking Union Medical College Hospital from 1983 to 2002 were reviewed.

Results: The incidence of AWE was 1.

[Benign ovarian tumor with ascites and high serum levels of CA125: report of 3 cases].

Objective: To describe the clinical characteristics of benign ovarian tumor/mass which manifest as pelvic mass, ascites and high serum levels of CA(125).

Method: Clinical data of 3 cases of such disease in Peking Union Medical College Hospital were analyzed and relevant literatures were reviewed.

Results: The patients all were misdiagnosed as ovarian carcinoma, and operations designed for malignant ovarian tumor were performed on all of them.