Changes in Nitric Oxide Releases of the Contralateral Acupoint during and after Laser Acupuncture at Bilateral Same-Name Acupoints in Human.

Objective: The purpose of the study was to examine the effects of laser acupuncture (LA) at right Neiguan (RPC6)/left Neiguan (LPC6) acupoints on the releases of nitric oxide (NO) in the treated and contralateral/nontreated PC6, compared to the nonacupoint control area.

Methods: 24 mW LA at RPC6, LPC6, and nonacupoint in 22 healthy subjects for 40 min: sterilized dialysis tube was taped to the nontreated PC6/nonacupoint during the treatment and immediately taped to the treated and nontreated PC6/nonacupoint after LA removal. NO-scavenging compound was injected into the tube for 40 min to absorb the molecular which was tested by spectrophotometry in a blinded fashion.

Effects of different-intensity laser acupuncture at two adjacent same-meridian acupoints on nitric oxide and soluble guanylate cyclase releases in human.

Objectives: The aim of this study was to detect the influences of LA at nonacupoint and two adjacent acupoints of pericardium meridian on the releases of NO and sGC in 20 healthy subjects.

Methods: Different intensities (12, 24, 48 mW) of infrared laser were used for irradiating Jianshi (PC5), Ximen (PC4) acupoints and nonacupoint for 20, 40 minutes, respectively. Semi-circular tubes were taped to the skin surface and filled with NO-scavenging compound for 20 minutes to capture NO and sGC, which were measured using spectrophotometry in a blinded fashion.

[Comparative study of time-correlated temperature and back-scattered light intensity for human Hegu acupoint and non-acupoint tissue irradiated by near-infrared laser].

Characteristics and differences of temperature and back-scattered light intensity in different depths of 0.2, 0.4, 0.

[In vivo reflectance spectroscopy study of different clearing agents on human skin optical clearing].

The changes of skin tissue reflectance spectroscopy before and after being treated with the optical clearing agents of three different types of optical clearing within the wavelength rang of 400-1 000 nm, and the degree of changes in reflectance spectroscopy of each group skin during 0-60 min at 580 nm in vivo were real-time dynamically researched. The reflectance spectroscopy of skin tissue before and after being dealt by the optical clearing agents of glycerol, glucose and propylene glycol was measured using a USB-4000 fiber spectrophotometer at 0, 10, 20, 30, 40, 50 and 60 min. The results showed that the reflectance spectral intensity was distinctly decreased, but the reflectance was significantly increased gradually with the time prolonged.

[Reflectance spectroscopy study of low-frequency ultrasound and glycerol on skin optical clearing].

In order to find a non-invasive way to improve the efficacy of skin optical clearing with topically applied optical clearing agents, the authors researched the changes of reflectance spectroscopy of skin tissues before and after being dealt by low-frequency ultrasound and osmotic active chemical agents within the wavelength range of 400-860 nm, and the degree of changes in reflectance spectroscopy of each group skin during 0-15 min and 15-30 min at 580 nm. The measurements were performed using a AvaSpec-2048 optical fiber spectrometer with integrating-sphere setup. The results of measurements showed that there were a few changes of the reflectance spectroscopy of skin tissues in the control group (Group 1) (The skin tissue was dealt with nothing.

[Comparative study of reflectance spectroscopy of human laogong acupoint and non-acupoint tissues irradiated by near-infrared laser].

Characteristics and differences of reflectance spectroscopy of human Laogong acupoint and non-acupoint tissues before and after irradiation by different power of laser were studied in the spectral range from 400 to 1 000 nm. A wavelength 808 nm semiconductor laser was used for irradiation at the power of 20, 50 and 100 mW for ten minutes. Reflectance spectra of human Laogong acupoint and non-acupoint tissues were measured by using an AvaSpec-2048 optical fiber spectroscopy with an integrating sphere attachment.

[Gastric cancer detection using kubelka-Munk spectral function of DNA and protein absorption bands].

Differential diagnosis for epithelial tissues of normal human gastric, undifferentiation gastric adenocarcinoma, gastric squamous cell carcinomas, and poorly differentiated gastric adenocarcinoma were studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 250 to 650 nm, pathological changes of gastric epithelial tissues induced that there were significant differences in the averaged value of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log[f(r infinity)] of the DNA absorption bands at 260 nm between epithelial tissues of normal human stomach and human undifferentiation gastric cancer, between epithelial tissues of normal human stomach and human gastric squamous cell carcinomas, and between epithelial tissues of normal human stomach and human poorly differentiated cancer.

[Colon adenoma detection using Kubelka-Munk spectral function of DNA and protein bands].

Differential diagnosis of human colon adenoma was studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 590 to 1 064 nm pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log [f(r infinity)] of the DNA absorption bands at 260 nm between normal and adenomatous colon epithelial tissues, and the differences were 218% (p < 0.

[Superficial bladder cancer detection using diffuse reflectance spectral ratio R540/R575 of oxygenated hemoglobin bands].

A low-cost, fast, and noninvasive method for early diagnosis of malignant lesions of mucosa tissue based on diffuse reflectance spectra was applied in the study of the optical biopsy of superficial human bladder cancer. In the present paper, differential diagnosis of superficial human bladder cancer was studied using the diffuse reflectance spectral ratio (R540/R575) of the oxygenated hemoglobin absorption bands at 540 and 575 nm in vitro. Diffuse reflectance spectra for mucosa/submucosa tissues of normal bladder and superficial bladder cancer were measured using a spectrophotometer with an integrating sphere attachment.

Novel high-throughput profiling of human transcription factors and its use for systematic pathway mapping.

Transcription factors (TFs) are crucial components of regulatory networks that control gene transcription. Current TF assays are limited to the analysis of a single TF or require TF-specific antibodies. Here we report the Single Primer Amplification assisted Oligonucleotide Array-based Transcription Factor Assay (SPA-OATFA) which can directly analyze the binding activities of 240 human TFs simultaneously.

[Absorption and scattering characteristics of human benign prostatic hyperplasia tissue with Ti: sapphire laser irradiation in vitro].

The optical properties and their differences of human benign prostatic hyperplasia (BPH) tissues removed using transurethral plasma kinetic resection of the prostate (PKRP) and transurethral vaporization of the prostate (TUVP) at 640, 660, 680, 700, 720, 740, 760, 780, 800, 820, 840, 860 and 880 nm of Ti: Sapphire laser were studied in vitro. The measurements were performed using a double-integrating-sphere setup, and the absorption and scattering properties were assessed using the inverse adding-doubling method. The results of measurement showed that the absorption coefficients and reduced scattering coefficients of BPH tissues removed using PKRP and TURP obviously decreased with the increase in the wavelength for thirteen different laser wavelengths.

[Canceration and thermal coagulation of human liver induced changes in the absorption and scattering properties of liver-tissue at near infrared in vitro].

Canceration and thermal coagulation of human liver induced changes in the absorption and scattering properties of liver tissue at 710, 730, 750, 77, 790, 810, 830, 850, 870 and 890 nm of Ti: sapphire laser were studied in vitro. The measurements were performed using a double-integrating-sphere setup, and the absorption and scattering properties were assessed from these measurements using the inverse adding-doubling method. The results of measurement showed that canceration of liver induced significant decrease in the absorption coefficients of liver tissue, and the maximum change in the absorption coefficients is 86.

[Comparison of light attenuation characteristics and optical penetration depths between native and coagulated human liver tissues].

A double-integrating-spheres and IAD method were used to study the differences in the optical penetration depths (OPDs) and light attenuation (LA) native and coagulated human liver tumors and liver tissues at the wavelengths of 680, 720, 780, 810, 850 and 890 nm of Ti: Sapphire laser. The results of measurement showed that the OPDs for native and coagulated human liver tumors and liver tissues at six different wavelengths obviously increase with increasing laser wavelength, the OPDs of coagulated human liver tumors and liver tissues at six different wavelengths were significantly smaller than that of native human liver tumors and liver tissues at the same wavelength respectively (P<0.05), and the OPDs of native and coagulated human liver tumors at six different wavelengths were significantly bigger than that of native and coagulated human liver tissues at the same wavelength respectively (P<0.

Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA).

Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data.

[Optical properties of human normal small intestine tissue with theoretical model of optics about biological tissues at Ar+ laser and 532 nm laser and their linearly polarized laser irradiation in vitro].

A double-integrating-spheres system, basic principle of measuring technology of ray radiation, and optical model of biological tissues were used for the study. Optical properties of human normal small intestine tissue at 476.5, 488, 496.

[Optical properties of human normal bladder tissue at five different wavelengths of laser and their linearly polarized laser irradiation in vitro].

A double-integrating-spheres system, the basic principle of measuring technology of radiation, and an optical model of biological tissues were used for the study. Optical properties of human normal bladder tissue at 476.5, 488, 496.