[Apoptosis of hepatocellular induced by active ingredients of Zanthoxyli Radix by Raman spectroscopy].

The apoptosis of mono-hepatocellular induced by the active ingredients of the Zanthoxyli Radix was investigated using laser Raman spectroscopy. Hepatoma cells (BEL-7404) were treated with 10 mg•L⁻¹ nitidine chloride and 3 g•L⁻¹ the extracts of Zanthoxyli Radix, respectively, then were divided into two parts, one for fluorescence staining, the other for determination of Raman spectroscopy. The acquired spectra were then processed by background elimination, smoothing, and normalization.

New C13 lipids from the marine-derived fungus Trichoderma harzianum.

Chemical examination of the fermentation broth of a sponge-associated fungus Trichoderma harzinum HMS-15-3 led to the isolation of four pairs of new C13 lipid enantiomers namely harzianumols A-H (1a-4b). Their structures were elucidated on the basis of extensive spectroscopic (IR, MS, 1D, and 2D NMR) data analysis, including the modified Mosher's method for the assignment of their absolute configurations. The new compounds were evaluated for antihyperlipidemic effects in HepG2 cells.

[GC-MS analysis of essential oil from Curcuma aromatica rhizome of different growth periods].

Objective: To analyze the essential oil from the rhizome of Curcuma aromatica of different growth periods, and to provide the scientific reference for reasonable cultivation and quality control of this plant.

Methods: The essential oil was extracted by hydrodistillation and analyzed with GC-MS. The relative contents were determined with area normalization method.

Losartan affects the substrate for atrial fibrillation maintenance in a rabbit model.

Background: Atrial fibrosis causes abnormal conduction through the atria, creating a substrate for atrial fibrillation (AF). In a rabbit model, rapid atrial pacing produces significant atrial fibrosis and the substrate for AF maintenance. This atrial remodeling is a potential therapeutic target.

[Effect of Jinhuang Fuzheng powder on cytokines of immunosuppressive mice with protein antibody micro-array].

Objective: To observe the effect of Jinhuang Fuzheng powder on cytokines of immunosuppressive mice by using protein antibody micro-array.

Method: The immunosuppressive mice model was established by subcutaneously injecting cyclophosphamide. Rats were orally administered with low, middle and high dose of Huangjin Fuzheng powder for 10 days, and fasted for 12 hours after the final administration.

Simultaneous determination of flavonoids, isochlorogenic acids and triterpenoids in Ilex hainanensis Using high performance liquid chromatography coupled with diode array and evaporative light scattering detection.

A high performance liquid chromatography coupled with diode array and evaporative light scattering detection (HPLC-DAD-ELSD) method for simultaneous determination of eight major bioactive compounds including two flavonoids (rutin and eriodictyol-7-O-β-D-glucopyranoside), two isochlorogenic acids (isochlorogenic acid A and isochlorogenic acid C) and four triterpenoids (ilexhainanoside D, ilexsaponin A1, ilexgenin A and ursolic acid) in Ilex hainanensis has been developed for the first time. The 283 nm wavelength was chosen for determination of two flavonoids and two isochlorogenic acids. ELSD was applied to determine four triterpenoids.

[Chemical constituents from Ilex hainanensis].

Objective: To investigate the chemical constituents from Ilex hainanensis.

Methods: The compounds were isolated and purified by various chromatographic techniques. The structures were identified by mass and NMR spectral data.

Gene expression profiling of nephrotoxicity from copper nanoparticles in rats after repeated oral administration.

The goal of this study was to investigate the mechanisms of nanocopper-induced nephrotoxicity by analyzing renal gene expression profiles phenotypically anchored to conventional toxicological outcomes. Male Wistar rats were given nanocopper (50, 100, 200 mg/kg) and microcopper (200 mg/kg) at different doses for 5 days. We found nanocopper can induce widespread renal proximal tubule necrosis in rat kidneys with blood urea nitrogen and creatinine increase.

[Application of two FTIR pattern recognition methods to the Zanthoxylum nitidum geographical origins determination].

In the present work, the authors explored a rapid method of the Zanthoxylum nitidum geographical origins determination. Based on Fourier transform infrared spectroscopy (FTIR) technology, the band of 1 800-400 cm(-1) which is the IR fingerprint of Zanthoxylum nitidum, the Fisher ratio and the soft independent modeling of class analogies (SIMCA) were used to build a classification model. Respectively, four kinds of Zanthoxylum nitidum in the Guangxi region were detected by the model, and the model was verified by calculating their recognition rate and rejection rate.

Synthesis, characterization and preliminary cytotoxicity evaluation of five lanthanide(III)-plumbagin complexes.

Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone, H-PLN) was isolated from Plumbago zeylanica, the anticancer traditional Chinese medicine (TCM). Five new lanthanide(III) complexes of deprotonated plumbagin: [Y(PLN)(3)(H(2)O)(2)] (1), [La(PLN)(3)(H(2)O)(2)] (2), [Sm(PLN)(3)(H(2)O)(2)]⋅H(2)O (3), [Gd(PLN)(3)(H(2)O)(2)] (4), and [Dy(PLN)(3)(H(2)O)(2)] (5) were synthesized by the reaction of plumbagin with the corresponding lanthanide salts, in amounts equal to ligand/metal molar ratio of 3:1. The PLN-lanthanide(III) complexes were characterized by different physicochemical methods: elemental analyses, UV-visible, IR and (1)H NMR and ESI-MS (electrospray ionization mass spectrum) as well as TGA (thermogravimetric analysis).

Potential new inorganic antitumour agents from combining the anticancer traditional Chinese medicine (TCM) matrine with Ga(III), Au(III), Sn(IV) ions, and DNA binding studies.

Three new compounds of Ga(III), Au(III), Sn(IV) with matrine (MT), [H-MT][GaCl(4)] (1), [H-MT][AuCl(4)] (2) and [Sn(H-MT)Cl(5)] (3), have been synthesized and characterized by elemental analysis, IR, ESI-MS and single crystal X-ray diffraction methods. The crystal structural analyses indicate that 1 and 2 are ionic compounds, whereas 3 is a tin(IV) complex formed by the monodentate MT via its carbonyl oxygen atom of MT coordinating to Sn(IV). Their in vitro cytotoxicity towards eight selected tumour cell lines has been evaluated by MTT (3-[4,5-Dimentylthiazole-2-yl]-2,5-diphenpyltetra-zolium bromide) method, and compounds 1 and 2 exhibit enhanced activity, such as 1 to SW480, 2 to HeLa, HepG2 and MCF-7, which exceeds matrine and cisplatin, and display synergistic contribution of their components.

[Hypoglycemic effect of extracts of cactus pear fruit polysaccharide in rats].

Objective: To study the hypoglycemic effect and mechanism of the extracts of cactus pear fruit polysaccharide (CPFP) in diabetic rats induced by streptozotocin (STZ).

Methods: The diabetic rats were induced by STZ in SD rats, and randomly divided into model group, insulin group,cactus pear juice group, high dose CPFP group,low dose CPFP group. The experimental rats were administrated for 8 weeks.

[Comparisons of volatile components in different parts of three species of Rhizoma Curcumae].

Objective: To provide the scientific basis for the reasonable development and use of the leaves of Rhizoma Curcumae.

Methods: To extract the volatile oil from roots and leaves of three species Rhizoma Curcumae by steam distillation and isolate these products with the method of gas chromatography-mass spectrum. Taking Nist98 standard mass spectrometer database and contrast as control, the structure of the mass spectrogram was identified, and the relative contents of the components were determined with area normalization method.

Cytotoxicity of the traditional chinese medicine (TCM) plumbagin in its copper chemistry.

The anticancer traditional Chinese medicine (TCM), plumbagin (PLN), was isolated from Plumbago Zeylanica. Reaction of plumbagin with Cu(II) salt, afforded [Cu(PLN)(2)].2H(2)O (1).

Divalent later transition metal complexes of the traditional chinese medicine (TCM) liriodenine: coordination chemistry, cytotoxicity and DNA binding studies.

Liriodenine (L), a natural alkaloid, was isolated as an active component from the anticancer traditional Chinese medicine (TCM), Zanthoxylum nitidum. It reacted with Mn(II), Fe(II), Co(II) and Zn(II) to afford four metal complexes: [MnCl(2)(L)(2)] (1), [FeCl(2)(L)(2)] (2), [Co(L)(2)(H(2)O)(2).Co(L)(2)(CH(3)CH(2)OH)(2)](ClO(4))(4) (3), and [Zn(2)(L)(2)(mu(2)-Cl)(2)Cl(2)] (4), which were characterized by elemental analysis, IR, ESI-MS.

[Antitumor effect of polysaccharides from cactus pear fruit in S180-bearing mice].

Background & Objective: Polysaccharide components of some traditional Chinese medicine have certain antitumor effects and can promote immune responses. Extractions from cactus pear fruit can inhibit the proliferation of cervical cancer, ovary cancer and bladder cancer cells, and suppress the growth of ovarian cancer in mice. This study was to observe the antitumor effect of polysaccharides extracted from cactus pear fruit in S180-bearing mice.

[Effect of octacostyl alcohol preparation on blood free radical metabolism and cardiac endocrine function of rats].

Objective: To evaluate the effect of octacostyl alcohol preparation on the motor function, blood free radical metabolism and cardiac endocrine function of rats.

Methods: A rat model of exercise fatigue was used for this study. The rats were made to perform continuous exhaustive exercise, and the total exercise time of the rats till exhaustion was recorded followed by examination of the serum superoxide dismutase (SOD), malonaldehyde (MDA), plasma endothelin (ET), calcitonin gene-related peptide (CGRP), and atrial natriuretic peptide (ANP).

[Overview pharmacokinetic about traditional Chinese medicine in recent 10 years].

This article summarized the progresses of pharmacokinetic about traditional Chinese medicine (TCM) in recent 10 years. Reports indicated that the studies of pharmacokinetic about TCM were in the stage of exploratory. Many factors restricted the progresses of pharmacokinetic such as complexity of medicine components, multi-target of drug effect and imperfect of evaluation methods.

[Protective effect of Jinhuang-1 on acute respiratory distress syndrome rats induced by oleic acid].

Objective: To observe the protective effect of Jinhuang-1 (JH-1) on oleic acid-induced acute respiratory distress syndrome (ARDS) in rats and provide a basis to clinical application.

Method: Oleic acid was injected into the tail vein of rats and JH-1 were administered to rats. After four days, their blood were collected for the blood gas analysis.