Seipin mutation at glycosylation sites activates autophagy in transfected cells via abnormal large lipid droplets generation.

Aim: Seipin is a protein that resides in endoplasmic reticulum, and involved in both lipid metabolic disorders and motor neuropathy. The aim of this study was to investigate the effects of mutant seipin on autophagy system and the morphology of lipid droplets in vitro.

Methods: HEK-293, H1299 and MES23.