Publications by authors named "Hua-Zhen Pan"
We investigate the correlation between the glycosylation modified prion proteins and apoptosis. The wild-type PRNP gene and four PRNP gene glycosylated mutants were transiently expressed in HeLa cells. The effect of apoptosis induced by PrP mutants was confirmed by MTT assay, Hochest staining, Annexin-V staining and PI staining.
View Article and Find Full Text PDFTo study the biological function of the N-glycosylation modification of prion proteins (PrP), various eukaryotic expression vectors for the mutants with N-glycosylation modification of human PrP had been constructed and expressed. With site-direct mutation technique, human PRNP gene was mutated and the obtained mutants were subcloned into eukaryotic expressing plasmid pcDNA3.1 and transiently expressed in Hela cervical adenocarcinoma cell.
View Article and Find Full Text PDFArticle Synopsis
- Selenium can have a protective role against cancer at low concentrations but a direct tumor-fighting effect at higher doses, though the exact mechanisms are not fully understood.
- In experiments, a concentration of 20 micromol/l of Na(2)SeO(3) significantly inhibited the growth of NB4 cells and induced changes suggesting cell death, while a lower concentration of 2 micromol/l had no such effects.
- Gene expression analysis revealed that different concentrations of selenium lead to distinct changes in gene regulation, highlighting the complexity of its anticancer mechanisms and suggesting potential pathways for leukemia treatment.
[GPI-Pr Deficiency and Apoptosis of PNH Granulocytes].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
September 2001
Article Synopsis
- - The study aimed to examine how Glycosyl phosphatidylinositol anchored proteins affect apoptosis in cells from patients with paroxysmal nocturnal hemoglobinuria (PNH) compared to normal controls.
- - Peripheral granulocytes from 10 PNH patients and 10 healthy individuals were analyzed, revealing that normal cells showed higher apoptosis rates (78.6%) than PNH cells (39.5%) when subjected to serum starvation.
- - Further analysis indicated that PNH granulocytes are relatively resistant to apoptosis, as evidenced by lower percentages of PI positivity and DNA fragmentation, and this resistance does not appear to be linked to CD59 deficiency levels.
[A colorimetric method of HEC toxin hemolysis test for diagnosis of paroxysmal nocturnal hemoglobinuria].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2002
Article Synopsis
- The study aims to develop a colorimetric method using HEC toxin for diagnosing paroxysmal nocturnal hemoglobinuria (PNH).
- Researchers treated RBCs from normal individuals and PNH/non-PNH anemia patients with HEC toxin and measured hemolysis through absorbance at 630 nm.
- The results showed that PNH patients' RBCs resisted hemolysis, validating the method's potential as a simple and reliable diagnostic tool for PNH.