[Development of the real-time reverse transcription-polymerase chain reaction assay for the rapid detection of rubella virus].

Objective: A new TaqMan probe based real-time assay was developed to rapid detection of rubella virus.

Methods: The specific primer pair and probe were designed within the conserved P150 gene of rubella virus and the PCR reactive condition was optimized to improve the sensitivity and specificity of the assay. Clinical specimens collected from two outbreaks were detected by the developed assay.