Combined effects of thermal environment and gene regulation on metabolic enzyme activities of major metabolic tissues in a winter-breeding amphibian.

Variations in thermal environments can provoke diverse physiological responses in amphibians. Despite extensive studies on the thermal sensitivity of amphibian metabolic physiology, including enzyme activity at different temperatures, the rationale for selecting specific metabolic enzymes and their relationship with gene expression remains unclear. Cytochrome c oxidase (CCO), succinate dehydrogenase (SDH), and lactate dehydrogenase (LDH) are key metabolic enzymes within the primary metabolic regulatory tissues of animals.

Two complete mitochondrial genomes of (Anura: Megophryidae: Megophryinae): characteristics and phylogenetic implications.

The Chinese horned toads, (Boulenger, 1899) and (Pope, 1929), are two captivating species within the family Megophryidae, which inhabit the mountainous streams in the Eastern of China. In this study, two new complete mitochondrial genomes of and were sequenced, assembled, and annotated using next-generation sequencing. The length of mitochondrial genomes of and was 16,597 and 17,921 bp, respectively, with both containing 13 protein coding genes, 22 tRNA genes, two rRNA genes, and one putative control region.

[Effect of Down-Regulation of ANRIL on Proliferation and Apoptosis of Kasumi-1 Cells and Its Potential Mechanism].

Objective: To investigate the down-regulation of ANRIL (Antisense non-coding RNA in the INK4 Locus) effects on proliferation and apoptosis of Kasumi-1 cells and its related molecular mechanism.

Methods: Recombinant lentivirus was used to construct ANRIL down-regulation Kasumi-1 cells (sh-ANRIL group) and its control cells (sh-NC group). A fluorescence microscope was used to observe the transfection efficiency, RT-qPCR was used to detect knockdown efficiency and ANRIL expression in PBMCs and MBMCs of patients with acute myeloid leukemia (AML).

Microbial Diversity of the Chinese Tiger Frog () on Healthy versus Ulcerated Skin.

The Chinese tiger frog () is extensively farmed in southern China. Due to cramped living conditions, skin diseases are prevalent among unhealthy tiger frogs which thereby affects their welfare. In this study, the differences in microbiota present on healthy versus ulcerated skin were examined using 16S rRNA sequences.

Ecotoxicity assessment of triphenyl phosphate (TPhP) exposure in Hoplobatrachus rugulosus tadpoles.

Triphenyl phosphate (TPhP), a widely used aromatic organophosphate flame retardant, is known to accumulate in organisms through water, air, and soil, consequently, causing toxicity. This study is the first to evaluate the acute and sub-chronic toxicities of TPhP to amphibians. In the acute toxicity analysis, the 96-h median lethal concentration (LC) for GS35 Hoplobatrachus rugulosus tadpoles was 2.

Advertisement calls of the lesser spiny frog Quasipaa exilispinosa (Liu and Hu, 1975) (Anura: Dicroglossidae).

There are currently only eleven species of Quasipaa (Anura: Dicroglossidae) distributed in southern and southwestern China to central Vietnam, southeastern Thailand, and southwestern Cambodia and presumably also in Laos (Frost 2020). Eight species of Quasipaa are currently known in China: Q. boulengeri (Günther 1889), Q.

Two complete mitochondrial genomes of the black-spotted stout newt () and their relative phylogenetics relationship with other Salamandridae.

The black-spotted stout newt () is widely distributed in the mountains of southeastern China. In this study, we sequenced and determined the complete mitochondrial genome (mitogenomes) of two samples collected in Mount Wuyi. The assembled mitogenomes were 16,298 bp and 16,301 bp in length, and contained 13 protein-coding genes (PCGs), two ribosomal RNA genes, 22 transfer RNA genes, one non-coding region, and one control region.