Construction of an effective m1 GS ribozyme targeting HCMV UL97 mRNA segment in vitro.

A sequence-specific M1GS ribozyme (M1-T3) was constructed by covalently linking an oligonucleotide (guide sequence,GS) to the 3' terminus of M1 RNA ,the catalytic subunit of RNase P from Escherichia coli. The engineered ribozyme is targeted to the mRNA sequence encoding a protein kinase (UL97) of HCMV and could effectively cleave the mRNA segment in vitro. Further studies about the significance of some structural elements in the M1 GS (e.

[Determination of trace barium in mineral water with oxygen enriched air-acetylene flame in atomic absorption spectrometry].

In this paper, the determination of Ba using oxygen enriched air-acetylene flame in AAS has been studied and the effect of acetylene flow, oxygen flow and the ratio of O2/C2H2 on the absorbance have been compared. When the amount of oxygen is 52% in mixed gas and the ratio of O2/C2H2 is about 0.72, higher sensitivity for Ba can be obtained.