Periodontal Commensals and Pathogens Differentially Modulate Immuno-Inflammatory Response in Human Oral Keratinocytes.

Objective: To investigate the immunoinflammatory response in the crosstalk of human oral keratinocytes (HOKs) with selected periodontal commensals and pathogens.

Methods: Four representative viable oral bacteria, including periodontal commensals (Streptococcus mutans, Sm; and Actinomyces israelii, Ai) and pathogens (Aggregatibacter actinomycetemcomitans, Aa; and Porphyromonas gingivalis, Pg), were selected. A viable bacteria-HOKs interactive model was tested under various conditions of oxygen, antibiotics, duration and multiplicity of infection (MOI).

[Mechanism of advanced glycation end-products in the inducement of apoptosis of human gingival fibroblast and related effect of puerarin in the process].

Objective: To observe the effect of synthesized advanced glycation end-products (AGE) on reactive oxygen species formation and apoptosis of the cultured human gingival fibroblast and investigate the potential mechanisms of AGE in the modification of periodontal impairment.

Methods: AGE products with different concentrations [0, 50, 150 mg/L AGE-human serum albumin (AGE-HSA)] were incubated with human gingival fibroblast for 48 h, respestively. Flow cytometry was used to detect intracellular reactive oxygen species and cell apoptosis.