Impacts of reproductive systems on grapevine genome and breeding.

Diversified reproductive systems can be observed in the plant kingdom and applied in crop breeding; however, their impacts on crop genomic variation and breeding remain unclear. Grapevine (Vitis vinifera L.), a widely planted fruit tree, underwent a shift from dioecism to monoecism during domestication and involves crossing, self-pollination, and clonal propagation for its cultivation.

Integration of small needle knife in treating inflammatory factors, Th17 cell function, and magnetic resonance imaging evaluation in osteoporosis complicated by compression fractures in elderly patients.

Objective: The incidence of osteoporosis complicated by vertebral compression fractures (OVCF) has been increasing annually, and percutaneous vertebroplasty (PVP) is the main surgical approach for it. It was to evaluate the use of a small needle knife (SNF) in conjunction with PVP to assess changes in inflammatory response, Th17 cell function, and magnetic resonance imaging (MRI) parameters in OVCF patients.

Methods: A retrospective analysis was conducted using the clinical data of 138 patients with OVCF, who were categorized into PVP group (n = 65) and PVP + SNF group (n = 73).

EHF promotes liver cancer progression by meditating IL-6 secretion through transcription regulation of KDM2B in TAMs.

Background: Macrophages are key immune cell types in liver, which are thought to be involved in tumor development. Recent studies indicated that TAMs exhibit M2 phenotypes. However, the mechanism of macrophages related to tumor progression in liver cancer is largely unknown.

Metagenomic next-generation sequencing of bronchoalveolar lavage fluid samples offers diagnostic utility in bacteriologically negative pulmonary tuberculosis.

Rapid diagnosing Mycobacterium tuberculosis (M. tb) in patients with pulmonary tuberculosis (PTB) cases is critical, particularly in cases without bacteriologically confirmed disease, as it enables timely treatment initiation and can thus interrupt further disease transmission. In this study, the utility of metagenomic next-generation sequencing (mNGS) as a diagnostic tool was evaluated using samples of bronchoalveolar lavage fluid (BALF) samples from 300 bacteriologically negative PTB (BN-PTB) patients hospitalized from January 2020 through December 2023.

Value of Post-NAC CT-based Node-RADS score for Predicting residual lymph node metastasis and survival outcome of locally advanced gastric cancer.

Objectives: The Node Reporting and Data System (Node-RADS) provides structured and effective evaluation for lymph nodes in malignancies. This study aims to investigate its value in predicting residual lymph node metastasis (LNM) and survival outcome of locally advanced gastric cancer (LAGC).

Materials And Methods: This retrospective study included 118 patients with LAGC underwent neoadjuvant chemotherapy (NAC) and gastrectomy from April 2015 to June 2020.

SERPINH1 secretion by cancer-associated fibroblasts promotes hepatocellular carcinoma malignancy through SENP3-mediated SP1/SQLE pathway.

Cancer-associated fibroblasts (CAFs) have garnered significant attention due to their ability to shape the tumor microenvironment, thereby facilitating tumor progression and metastasis. Serpin peptidase inhibitor clade H member 1 (SERPINH1) is known for its role in the proper folding and secretion of collagen. However, its biological significance in hepatocellular carcinoma (HCC) remains unclear.

A potential tumor suppressor role of PLK2 in glioblastoma.

Glioblastoma (GBM) is a highly malignant brain tumor with limited treatment options. Polo-like kinase 2 (PLK2), a member of the polo-like kinase family, has been variably implicated in cancer, but its role in GBM has not been fully elucidated. We utilized RNA-seq data from multiple databases, including Gene Expression Omnibus (GEO), the Cancer Genome Atlas (TCGA), and the Chinese Glioma Genome Atlas (CGGA), and conducted experiments on human glioma cell lines to explore PLK2's expression and function.

The genomic and epigenomic landscapes of hemizygous genes across crops with contrasting reproductive systems.

Hemizygous genes, which are present on only one of the two homologous chromosomes of diploid organisms, have been mainly studied in the context of sex chromosomes and sex-linked genes. However, these genes can also occur on the autosomes of diploid plants due to structural variants (SVs), such as a deletion/insertion of one allele, and this phenomenon largely unexplored in plants. Here, we investigated the genomic and epigenomic landscapes of hemizygous genes across 22 genomes with varying propagation histories: eleven clonal lineages, seven outcrossed samples, and four inbred and putatively homozygous genomes.

Flexible Semi-synthesis of UFM1-Propargylamine by Aminolysis with Valine-Propargylamine.

We report a novel semi-synthetic strategy of UFM1-propargylamine by N-S acyl transfer to give UFM1R thioester and subsequent aminolysis with valine-propargylamine. The use of the valine-propargylamine molecule overcomes the inability of the valine site to efficiently undergo the N-S acyl transfer. This strategy benefits from the availability of raw materials for bulk expression, and the resulting probe shows high selectivity toward UFM1-specific proteases in buffers and cell lysates.

Genomic predictions of invasiveness and adaptability of the cotton bollworm in response to climate change.

Agricultural pests cause enormous losses in annual agricultural production. Understanding the evolutionary responses and adaptive capacity of agricultural pests under climate change is crucial for establishing sustainable and environmentally friendly agricultural pest management. In this study, we integrate climate modeling and landscape genomics to investigate the distributional dynamics of the cotton bollworm (Helicoverpa armigera) in the adaptation to local environments and resilience to future climate change.

Modified TPPA combined with western blotting facilitates syphilis diagnosis in isolated reactive treponemal chemiluminescence immunoassay sera: a prospective cohort study.

Background: The challenge of dealing with isolated reactive treponemal chemiluminescence immunoassay (CIA) results in clinical practice has prompted the development of a more efficient algorithm for distinguishing true infection from false reactivity in isolated CIA sera.

Methods: A prospective cohort study was conducted at Wuhan Tongji Hospital, involving 119,002 individuals screened for syphilis using CIA from January 1, 2015, to January 6, 2017. Samples with reactive CIA results underwent simultaneous testing with the T.

PMAIP1-mediated glucose metabolism and its impact on the tumor microenvironment in breast cancer: Integration of multi-omics analysis and experimental validation.

Background: Glucose metabolism in breast cancer has a potential effect on tumor progression and is related to the immune microenvironment. Thus, this study aimed to develop a glucose metabolism-tumor microenvironment score to provide new perspectives on breast cancer treatment.

Method: Data were acquired from the Gene Expression Omnibus and UCSC Xena databases, and glucose-metabolism-related genes were acquired from the Gene Set Enrichment Analysis database.

Association between serum calcium level and the risk of acute kidney injury in ICU patients with subarachnoid hemorrhage: a retrospective cohort study.

Aim: This study aimed to evaluate the association between serum calcium level and the risk of acute kidney injury (AKI) in patients with subarachnoid hemorrhage (SAH).

Methods: In this retrospective cohort study, data on adults from the Medical Information Mart for Intensive Care (MIMIC-III and MIMIC-IV) databases, spanning from 2008 to 2019, were extracted. In the logistic regression models, confounding variables, including age, white blood cell (WBC), systolic blood pressure (SBP), heart rate, blood urea nitrogen (BUN), glucose, international normalized ratio (INR), and the Charlson Comorbidity Index (CCI), were finally adjusted by stepwise regression.

Prognostic factors and adjuvant chemotherapy efficacy in stage I gastric cancer patients: a retrospective analysis.

Background: Although stage I gastric cancer (GC) presents a favorable survival rate, outcomes for patients experiencing recurrence remain poor. This research focuses on assessing the prognosis and identifying risk factors for stage I GC patients, further assessing the necessity of adjuvant chemotherapy (AC).

Methods: The study involved a retrospective analysis of 902 patients with stage I GC who received curative resection from November 2010 to December 2020.

Peripheral T Lymphocyte Predicts the Prognosis of Gastric Cancer Patients Undergoing Radical Gastrectomy: A Multicenter Retrospective Cohort Study.

Objective: The aim of this study was to investigate the predictive value of peripheral lymphocyte subsets for prognosis of gastric cancer (GC) patients following radical gastrectomy.

Methods: Consecutive GC patients received curative resection and examined peripheral lymphocyte subsets in Hunan Cancer Hospital were enrolled as training cohort (n=231), and those from Wuhan Union Hospital and Wuhan Tongji Hospital were included as external validation cohort (n=159). The optimal cutoff values of lymphocyte subsets for overall survival (OS) in training cohort were determined by X-tile.

TRIM37 exacerbates cerebral ischemic injury by regulating the PPARγ/NF-κB pathway.

Ischemic stroke is the primary cause of mortality for individuals with disability worldwide. Tripartite motif 37 (TRIM37) plays multiple regulatory roles in various cellular processes. Our research aimed to investigate the effects of TRIM37 on the progression of ischemic stroke and its related mechanisms.

Association between the use of loop diuretics and prognosis in critically ill patients with acute kidney injury: a retrospective propensity score-matched cohort study.

Background: The role of loop diuretics in patients with acute kidney injury (AKI) is controversial. This study examined the association between the use of loop diuretics and prognosis in critically ill patients with AKI.

Methods: This study used data from the medical information mart for the intensive care IV database.

Grapevine pangenome facilitates trait genetics and genomic breeding.

Grapevine breeding is hindered by a limited understanding of the genetic basis of complex agronomic traits. This study constructs a graph-based pangenome reference (Grapepan v.1.

Comprehensive mendelian randomization reveals atrial fibrillation-breast cancer relationship and explores common druggable targets.

Background: Atrial fibrillation (AF) and breast cancer pose significant risks to human health. The reasons behind the concurrent occurrence of AF and breast cancer remain unclear, leading to complex treatment approaches. Mendelian Randomization (MR) analyses aim to offer genetic evidence supporting the causation of AF and breast cancer and to investigate common druggable genes associated with both conditions.

Integrative genomics reveals the polygenic basis of seedlessness in grapevine.

Seedlessness is a crucial quality trait in table grape (Vitis vinifera L.) breeding. However, the development of seeds involved intricate regulations, and the polygenic basis of seed abortion remains unclear.

GDF15 regulated by HDAC2 exerts suppressive effects on oxygen-glucose deprivation/reoxygenation-induced neuronal cell pyroptosis via the NLRP3 inflammasome.

Background: Pyroptosis, inflammation-related programed cell death mediated by NLRP3 inflammasome, is involved in the pathogenesis of cerebral hypoxic-ischemic injury. Our study aims to explore the biological role of growth differentiation factor (GDF)15 in oxygen-glucose deprivation/reoxygenation (OGD/R)-induced neuronal pyroptosis.

Methods: HT22 neurons were subjected to OGD/R to simulate cerebral hypoxic-ischemic injury.