Establishment of Real-Time PCR Method to Differentiate (Diptera, Psychodidae) from s.s. Based on Whole Mitochondrial Genome Analysis.

, considered a potential vector for visceral leishmaniasis (VL), is distributed in the southern Gansu and northern Sichuan regions in China. However, the high similarity in the morphology of and s.s.

Dual-energy CT for predicting serosal invasion in gastric cancer and subtype analysis.

Purpose: To predict the serosal invasion of gastric cancer (GC) using dual-energy CT (DECT)-based parameters and analyze the diagnostic performance according to different subtypes.

Methods: The patients were divided into the T1-3 group and T4a group. The irregular region of interest (ROI) was manually delineated on the largest cross-section of the lesion.

OCTN2 expression and function in the Sertoli cells of testes from patients with non-obstructive azoospermia.

Background: Among couples, male factors account for approximately 50% of infertility cases, with nonobstructive azoospermia (NOA) representing one of the most clinically common and severe categories of male infertility, affecting approximately 10-15% of patients. Currently, L-carnitine is clinically used to improve spermatogenesis by regulating Sertoli cell function. Multiple clinical trials have described the efficacy of L-carnitine in treating NOA.

Correction: Huang et al. Next Generation of Computationally Optimized Broadly Reactive HA Vaccines Elicited Cross-Reactive Immune Responses and Provided Protection against H1N1 Virus Infection. 2021, , 793.

The authors would like to make the following corrections to this published paper [...

Dynamic Screw Lead Accuracy Measurement: Research on the Compensation of the Workpiece Placed Away from the Ideal Position.

The screw, a critical element in a variety of transmission mechanisms, significantly influences the performance of the transmission process. Accurate measurement of screw lead is crucial for ensuring the quality of transmission equipment. However, the measurement process can be affected by the precision limits of the measuring instruments and the challenges of manual fine adjustments.

Research on energy-saving renovation of old oceanarium based on energy consumption monitoring technology.

This study takes an old oceanarium in Jiangsu Province, China, as a case study and monitors the energy consumption values related to electricity, water and gas consumption for the whole year before the renovation of the oceanarium. Based on the energy consumption monitoring data, an in-depth analysis of the energy consumption defects of the oceanarium before the renovation is conducted using energy audit technology. We combine the characteristics of the oceanarium itself according to: (1) the results of the building energy audit; (2) the results of the envelope calculation; and (3) the latest energy-saving retrofit technology.

Investigating immune dysregulation and hub genes in septic cardiomyopathy development.

Septic cardiomyopathy is a life-threatening heart dysfunction caused by severe infection. Considering the complexity of pathogenesis and high mortality, the identification of efficient biomarkers are needed to guide clinical practice. Based on multimicroarray analysis, this study aimed to explore the pathogenesis of septic cardiomyopathy and the related immune landscape.

COBRA N2 NA vaccines induce protective immune responses against influenza viral infection.

Influenza neuraminidase (NA) is a promising target for a broadly protective vaccine. In this study, the Computationally Optimized Broadly Reactive Antigen (COBRA) methodology was used to develop N2 NA vaccine candidates. The unique wild type (WT) N2 sequences of human and swine influenza strains isolated between 1957 and 2019 were used to design the COBRA N2-A NA vaccine, while the unique WT N2 sequences of human influenza strains isolated between 2000 and 2019 were used to design the COBRA N2-B NA vaccine.

The chloroplast-localized casein kinase II α subunit, CPCK2, negatively regulates plant innate immunity through promoting S-nitrosylation of SABP3.

The casein kinase II (CK2) complex consists of catalytic (α) and regulatory (β) subunits and is highly conserved throughout eukaryotes. Plant CK2 plays critical roles in multiple physiological processes; however, its function in plant immunity remains obscure. In this study, we demonstrated that the unique chloroplast-localized CK2 α subunit (CPCK2) is a negative regulator of Arabidopsis thaliana innate immunity.

DLCNBC-SA: a model for assessing axillary lymph node metastasis status in early breast cancer patients.

Background: Axillary lymph node (ALN) status is a crucial prognostic indicator for breast cancer metastasis, with manual interpretation of whole slide images (WSIs) being the current standard practice. However, this method is subjective and time-consuming. Recent advancements in deep learning-based methods for medical image analysis have shown promise in improving clinical diagnosis.

Development of a vibration-damping, sound-insulating, and heat-insulating porous sphere foam system and its application in green buildings.

With the development of green buildings, people pay more attention to the quality of the indoor sound environment. The air sound insulation performance of floors and exterior walls plays a key role in today's green buildings. The thermal performance of the enclosure structure's floor and exterior wall heat transfer resistance is an important factor in reducing building carbon emissions in green buildings.

Inactivated influenza virus vaccines expressing COBRA hemagglutinin elicited broadly reactive, long-lived protective antibodies.

The influenza viruses cause seasonal respiratory illness that affect millions of people globally every year. Prophylactic vaccines are the recommended method to prevent the breakout of influenza epidemics. One of the current commercial influenza vaccines consists of inactivated viruses that are selected months prior to the start of a new influenza season.

miR-4429 inhibits ccRCC proliferation, migration, and invasion by directly targeting CD274.

Clear cell renal cell carcinoma (ccRCC) is one of the most aggressive urological malignancies and a highly immunogenic cancer. Yet, its pathogenesis is still not fully understood. This study analyzed the role of the miR-320 family in ccRCC using bioinformatics algorithms and a series of in vitro experiments.

Targeted Restoration of GPX3 Attenuates Renal Ischemia/Reperfusion Injury by Balancing Selenoprotein Expression and Inhibiting ROS-mediated Mitochondrial Apoptosis.

Background: Renal ischemia/reperfusion (IR) injury is the leading cause of acute kidney injury in both autologous and transplanted kidneys. Low-level glutathione peroxidase 3 (GPX3) is associated with renal IR injury. The exact mechanism of targeted GPX3 restoration in renal IR injury has yet to be determined.

Reactive Oxygen Species-Responsive Dual Anti-Inflammatory and Antioxidative Nanoparticles for Anterior Uveitis.

Anterior uveitis (AU) is an immune-mediated inflammatory disease that results in iritis, cyclitis, glaucoma, cataracts, and even a loss of vision. The frequent and long-term administration of corticosteroid drugs is limited in the clinic owing to the side effects and patient noncompliance with the drugs. Therefore, specifically delivering drugs to inflammatory anterior segment tissues and reducing the topical application dosage of the drug are still a challenge.

Phosphorylation of Mad1 at serine 18 by Mps1 is required for the full virulence of rice blast fungus, Magnaporthe oryzae.

The spindle assembly checkpoint (SAC) proteins are conserved among eukaryotes safeguarding chromosome segregation fidelity during mitosis. However, their biological functions in plant-pathogenic fungi remain largely unknown. In this study, we found that the SAC protein MoMad1 in rice blast fungus (Magnaporthe oryzae) localizes on the nuclear envelope and is dispensable for M.

Inactivated recombinant influenza vaccine: the promising direction for the next generation of influenza vaccine.

Introduction: Vaccination is the most effective method to control the prevalence of seasonal influenza and the most widely used influenza vaccine is the inactivated influenza vaccine (IIV). Each season, the influenza vaccine must be updated to be most effective against current circulating variants. Therefore, developing a universal influenza vaccine (UIV) that can elicit both broad and durable protection is of the utmost importance.

Multi-COBRA hemagglutinin formulated with cGAMP microparticles elicit protective immune responses against influenza viruses.

Influenza viruses cause a common respiratory disease known as influenza. In humans, seasonal influenza viruses can lead to epidemics, with avian influenza viruses of particular concern because they can infect multiple species and lead to unpredictable and severe disease. Therefore, there is an urgent need for a universal influenza vaccine that provides protection against seasonal and pre-pandemic influenza virus strains.

MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis.

Background: Proteasome inhibitors (PIs) are one of the most important classes of drugs for the treatment of multiple myeloma (MM). However, almost all patients with MM develop PI resistance, resulting in therapeutic failure. Therefore, the mechanisms underlying PI resistance in MM require further investigation.